We narrowed to 13,691 results for: NTS

1. pX330-Flag-SpCas9-HF1 (without sgRNA; with silent mutations)

   Plasmid

   #126755

   Purpose

   Expression plasmid for human codon-optimized increased fidelity SpCas9-HF1 (without U6-sgRNA coding sequence, with silent mutations)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   SpCas9-HF1
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, Q926A

   Promoter

   CBh

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCEP4-myc-H2-Dd-VN (Y84C/C121S/A139C)

   Plasmid

   #135501

   Purpose

   Mammalian expression of VN-fused and myc-tagged H2-Dd (Y84C/C121S/A139C mutant)

   Depositor

   Erik Procko

   Article

   McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

   Insert

   H2-Dd (H2-D1 Mouse)
   

   Use

   Tags

   Extracellular c-myc epitope tag, Signal peptide f…

   Expression

   Mammalian

   Mutation

   Y84C/C121S/A139C

   Promoter

   CMV

   Available since

   Feb. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCEP4-myc-HLA-A2-VN (I52P)

   Plasmid

   #135486

   Purpose

   Mammalian expression of VN-fused and myc-tagged HLA-A*02:01 (I52P mutant)

   Depositor

   Erik Procko

   Article

   McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

   Insert

   HLA-A*02:01 (HLA-A Human)
   

   Use

   Tags

   Extracellular c-myc epitope tag, Signal peptide f…

   Expression

   Mammalian

   Mutation

   I52P

   Promoter

   CMV

   Available since

   Feb. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCEP4-myc-HLA-A2-VN (Y59P)

   Plasmid

   #135492

   Purpose

   Mammalian expression of VN-fused and myc-tagged HLA-A*02:01 (Y59P mutant)

   Depositor

   Erik Procko

   Article

   McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

   Insert

   HLA-A*02:01 (HLA-A Human)
   

   Use

   Tags

   Extracellular c-myc epitope tag, Signal peptide f…

   Expression

   Mammalian

   Mutation

   Y59P

   Promoter

   CMV

   Available since

   Feb. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCEP4-myc-HLA-A2-VN (D61P)

   Plasmid

   #135493

   Purpose

   Mammalian expression of VN-fused and myc-tagged HLA-A*02:01 (D61P mutant)

   Depositor

   Erik Procko

   Article

   McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

   Insert

   HLA-A*02:01 (HLA-A Human)
   

   Use

   Tags

   Extracellular c-myc epitope tag, Signal peptide f…

   Expression

   Mammalian

   Mutation

   D61P

   Promoter

   CMV

   Available since

   Feb. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCEP4-myc-HLA-A2-VN (V67P)

   Plasmid

   #135494

   Purpose

   Mammalian expression of VN-fused and myc-tagged HLA-A*02:01 (V67P mutant)

   Depositor

   Erik Procko

   Article

   McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

   Insert

   HLA-A*02:01 (HLA-A Human)
   

   Use

   Tags

   Extracellular c-myc epitope tag, Signal peptide f…

   Expression

   Mammalian

   Mutation

   V67P

   Promoter

   CMV

   Available since

   Feb. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCEP4-myc-HLA-A2-VN (Y84P)

   Plasmid

   #135495

   Purpose

   Mammalian expression of VN-fused and myc-tagged HLA-A*02:01 (Y84P mutant)

   Depositor

   Erik Procko

   Article

   McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

   Insert

   HLA-A*02:01 (HLA-A Human)
   

   Use

   Tags

   Extracellular c-myc epitope tag, Signal peptide f…

   Expression

   Mammalian

   Mutation

   Y84P

   Promoter

   CMV

   Available since

   Feb. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCEP4-myc-HLA-A2-VN (E166P)

   Plasmid

   #135498

   Purpose

   Mammalian expression of VN-fused and myc-tagged HLA-A*02:01 (E166P mutant)

   Depositor

   Erik Procko

   Article

   McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

   Insert

   HLA-A*02:01 (HLA-A Human)
   

   Use

   Tags

   Extracellular c-myc epitope tag, Signal peptide f…

   Expression

   Mammalian

   Mutation

   E166P

   Promoter

   CMV

   Available since

   Feb. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. hKCNB1 L211P

   Plasmid

   #124487

   Purpose

   missense mutation in children with EOEE. HA tagged in C-terminus, pCIneo vector

   Depositor

   Federico Sesti

   Article

   Yu et al FASEB J. 2019 Dec;33(12):14680-14689. do

   Insert

   human KCNB1 (KCNB1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   changed leucine 211 to proline

   Promoter

   Available since

   Sept. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. hKCNB1 R312H

    Plasmid

    #124488

    Purpose

    missense mutation in children with EOEE. HA tagged in C-terminus, pCIneo vector

    Depositor

    Federico Sesti

    Article

    Yu et al FASEB J. 2019 Dec;33(12):14680-14689. do

    Insert

    human KCNB1 (KCNB1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed arginine 312 to histidine

    Promoter

    Available since

    Sept. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. hKCNB1 G381R

    Plasmid

    #124490

    Purpose

    missense mutation in children with EOEE. HA tagged in C-terminus, pCIneo vector

    Depositor

    Federico Sesti

    Article

    Yu et al FASEB J. 2019 Dec;33(12):14680-14689. do

    Insert

    human KCNB1 (KCNB1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed glycine at 381 to arginine

    Promoter

    Available since

    Sept. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. hKCNB1 F416L

    Plasmid

    #124491

    Purpose

    missense mutation in children with EOEE. HA tagged in C-terminus, pCIneo vector

    Depositor

    Federico Sesti

    Article

    Yu et al FASEB J. 2019 Dec;33(12):14680-14689. do

    Insert

    human KCNB1 (KCNB1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed phenyalanine 416 to leucine

    Promoter

    Available since

    Sept. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pIE-N-hα2M-H6

    Plasmid

    #128493

    Purpose

    Expresses human N-α2M (from S24 to E908) in insect cells. With C-t H6x tag.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

    Insert

    A2M (S24-E908) (A2M Human)
    

    Use

    Tags

    His tag (6x)

    Expression

    Insect

    Mutation

    Promoter

    IE1

    Available since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pS6-N-hα2M-H6

    Plasmid

    #128496

    Purpose

    Expresses human N-α2M (from G27 to A788) in mammalian cells. With C-t H6x tag.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

    Insert

    A2M (G27-A788) (A2M Human)
    

    Use

    Tags

    His tag (6x)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. hKCNB1 G379R

    Plasmid

    #124489

    Purpose

    missense mutation in children with EOEE. HA tagged in C-terminus, pCIneo vector

    Depositor

    Federico Sesti

    Article

    Yu et al FASEB J. 2019 Dec;33(12):14680-14689. do

    Insert

    human KCNB1 (KCNB1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed glycine at 379 to arginine

    Promoter

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMCh1750_S2F-IMCg_doxy-CMV_mChe-Cdc42E7

    Plasmid

    #118614

    Purpose

    expression of mChe-tagged cdc42E7 under doxy-inducible CMV promoter

    Depositor

    Marina Chekulaeva

    Article

    Ciolli Mattioli et al Nucleic Acids Res. 2018 Dec 22. pii: 525

    Insert

    cdc42E7 (Cdc42 Mouse)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    tetON CMV

    Available since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMCh1753_S2F-IMCg_doxy-CMV_mChe-Cdc42E7-E63'UTR

    Plasmid

    #118615

    Purpose

    expression of mChe-tagged cdc42E7-E6-3'UTR (swap) under doxy-inducible CMV promoter

    Depositor

    Marina Chekulaeva

    Article

    Ciolli Mattioli et al Nucleic Acids Res. 2018 Dec 22. pii: 525

    Insert

    cdc42E7-E6 3'UTR (Cdc42 Mouse)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    tetON CMV

    Available since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. FB026

    Plasmid

    #119711

    Purpose

    Transcriptional Unit (TU) for YFP expression, obtained by combining FB/GBparts FB007+GB0053+FB008 into pDGB3alpha1R. According to FungalBraid/GoldenBraid modular DNA assembly for ATMT

    Depositor

    Jose Marcos

    Article

    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

    Insert

    Promoter gpdA:YFP coding sequence:Terminator trpC
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLAP-BUBR1-3A

    Plasmid

    #114054

    Purpose

    transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

    Depositor

    Geert Kops

    Article

    Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

    Insert

    BUBR1-3A (BUB1B Human)
    

    Use

    Tags

    YFP-TEV-S- Human LAP

    Expression

    Mammalian

    Mutation

    C2823A and G2826A (siRNA-resistance), BUBR1-S670A…

    Promoter

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLAP-BUBR1-3D

    Plasmid

    #114055

    Purpose

    transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

    Depositor

    Geert Kops

    Article

    Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

    Insert

    BUBR1-3D (BUB1B Human)
    

    Use

    Tags

    YFP-TEV-S- Human LAP

    Expression

    Mammalian

    Mutation

    C2823A and G2826A (siRNA-resistance), BUBR1-S670D…

    Promoter

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLAP-MIS12-KARD-3D

    Plasmid

    #114058

    Purpose

    transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

    Depositor

    Geert Kops

    Article

    Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

    Insert

    MIS12-KARD-3D (BUB1B Human)
    

    Use

    Tags

    YFP-TEV-S- Human LAP

    Expression

    Mammalian

    Mutation

    BUBR1-S670D, S676D and T680D

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLAP-MIS12-KARD-3A

    Plasmid

    #114059

    Purpose

    transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

    Depositor

    Geert Kops

    Article

    Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

    Insert

    MIS12-KARD-3A (BUB1B Human)
    

    Use

    Tags

    YFP-TEV-S- Human LAP

    Expression

    Mammalian

    Mutation

    BUBR1-S670A, S676A and T680A

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pTE3314

    Plasmid

    #107530

    Purpose

    Expresses human codon optimized FnCpf1(RVR mutant) in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Insert

    hFnCpf1(RVR)
    

    Use

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    N607R, K613V, N617R

    Promoter

    CMV

    Available since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pUC19-Q6KIQ7

    Plasmid

    #103128

    Purpose

    Cas9 coding gene template with optimized sequence for human codon usage

    Depositor

    Duhee Bang

    Article

    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

    Insert

    Q6KIQ7(Cas9 coding gene from Mycoplasma mobile (strain ATCC 43663 / 163K / NCTC 11711))
    

    Use

    CRISPR; Cloning vector

    Tags

    Expression

    Mutation

    human codon-optimized

    Promoter

    Available since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pUC19-Q8DTE3

    Plasmid

    #103129

    Purpose

    Cas9 coding gene template with optimized sequence for human codon usage

    Depositor

    Duhee Bang

    Article

    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

    Insert

    Q8DTE3(Cas9 coding gene from Treponema denticola (strain ATCC 35405 / CIP 103919 / DSM 14222))
    

    Use

    CRISPR; Cloning vector

    Tags

    Expression

    Mutation

    human codon-optimized

    Promoter

    Available since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pUC19-D3UFL8

    Plasmid

    #103095

    Purpose

    Cas9 coding gene template with optimized sequence for human codon usage

    Depositor

    Duhee Bang

    Article

    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

    Insert

    D3UFL8(Cas9 coding gene from Helicobacter mustelae (strain ATCC 43772 / LMG 18044 / NCTC 12198 / 12198))
    

    Use

    CRISPR; Cloning vector

    Tags

    Expression

    Mutation

    human codon-optimized

    Promoter

    Available since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pTE3319

    Plasmid

    #107533

    Purpose

    Expresses human codon optimized MbCpf1(RR mutant) in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Insert

    hMbCpf1(RR)
    

    Use

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    N576R, K637R

    Promoter

    CMV

    Available since

    April 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pTE3316

    Plasmid

    #107532

    Purpose

    Expresses human codon optimized FnCpf1(RR mutant) in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Insert

    hFnCpf1(RR)
    

    Use

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    N607R, K671R

    Promoter

    CMV

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pTE3317

    Plasmid

    #107531

    Purpose

    Expresses human codon optimized MbCpf1(RVR mutant) in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Insert

    hMbCpf1(RVR)
    

    Use

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    N576R, K582V, N586R

    Promoter

    CMV

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pTE3179

    Plasmid

    #107528

    Purpose

    Expresses Mb crRNA and mCherry in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Inserts

    Mb crRNA
    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and human U6

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge