We narrowed to 8,394 results for: Dos;
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Plasmid#106204PurposeTight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertSF-iGluSnFR.A184S
UseAAVMutationGltI: A184S + mRuby3 fusionPromoterCAGAvailable SinceFeb. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
myr-FLAG-PIK3CA-pcw107
Plasmid#64605Purposewhen used to produce lentivirus, express physiological levels of insertDepositorAvailable SinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKK-mCherry-TEV
Plasmid#105779PurposeExpression of your protein of interest in fusion with red fluorescent protein at the N-terminus (cleavable by TEV), (PMID: 15558047). mCherry descends from the first truly monomeric red FP mRFP1.DepositorTypeEmpty backboneUseFlp-in competentTagsmCherry-TEVExpressionMammalianAvailable SinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLJM60-FLAG-SLC38A9.1 (delta110)
Plasmid#71861Purposelentiviral stable expressionDepositorInsertSLC38A9.1 (SLC38A9 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationcodon optimized, missing first 110 amino acidsPromoterCMVAvailable SinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
OCT4 GFP puro Donor 1
Plasmid#22209DepositorInsertSA-OCT-GFP-2APuro-PA (POU5F1 Human)
Available SinceOct. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
GFP-BRPF3
Plasmid#65383PurposeGFP-tagged BRD protein, which can be used to be expressed in mammalian cells.DepositorAvailable SinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKK-RNAi-nucEGFPmiR-TEV-mCherry
Plasmid#105812PurposeFunctional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear EGFP expression marker; your protein of interest with C-terminal mCherry.DepositorTypeEmpty backboneUseRNAi; Flp-in competentTagsTEV-mCherryExpressionMammalianAvailable SinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG-FLEX.SF-iGluSnFR.A184V.mRuby3
Plasmid#106205PurposeMedium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertSF-iGluSnFR.A184V
UseAAVMutationGltI: A184V + mRuby3 fusionPromoterCAGAvailable SinceFeb. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23 A82V 2014 EBOV + Mucin-Like Domain
Plasmid#86016PurposeEbola Virus (Makona) Glycoprotein in CMV driven mammalian expression vectorDepositorInsertMakona Ebola Virus Glycoprotein
ExpressionMammalianMutationChanged alanine 82 to valinePromoterCMV IEAvailable SinceJan. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
TV-hPITX3-forward
Plasmid#22073DepositorAvailable SinceOct. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-SBECN1W
Plasmid#107937PurposeAdeno-Associated viral (AAV) vector plasmid expressing mouse Beclin-1 (BECN1) in neuronsDepositorAvailable SinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKK-BI16-TEV-mCherry
Plasmid#105800PurposeConcomitant expression of two proteins. One protein is expressed with mCherry fusion tag (at the C-terminus), cleavable by TEV protease; second tag introduced by user.DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-mCherryExpressionMammalianAvailable SinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLEX307-IBV-3CL
Plasmid#160288PurposeAllows for constituitive mammalian expression of the IBV 3CL protease via transfection or lentivirusDepositorInsert3CL Protease
UseLentiviralExpressionMammalianPromoterEF1aAvailable SinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET22-His-TEV-DOT1L
Plasmid#89610PurposeBacterial expression vector for an N-terminally His-tagged (TEV-cleavable) version of human Dot1L residues 1-416DepositorAvailable SinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCK005_U6-Sp-sgRNA_UP-TdTomato_WPRE
Plasmid#85453PurposeLentiviral CRISPR system backbone bearing BsmBI site for S. pyogenes new guide RNAs and TdTomato.DepositorInsertU6-sgRNA-Backbone-UP-TdTomato
UseCRISPR and LentiviralTagsNLSExpressionMammalianMutationdeletion of AA123-365 in tdTomato (please see dep…Available SinceApril 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
Cbh_v5 AAV-saCBE C-terminal
Plasmid#137183PurposeAAV genome: expresses the C-terminal of S. aureus v5 AAV-CBE from the Cbh promoter, and U6-sgRNADepositorInsertv5 AAV-saCBE C-terminal
UseAAVPromoterCbhAvailable SinceJan. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
LentiRCas9-Lambda2
Plasmid#104184PurposeLentiviral transfer vector that carries U6-driven non-targeting sgRNA using a modified scaffold (Chen et al. Cell 2013) and CMV-driven PIN-dCas9. Derived from LentiCRISPR v2 (Zhang lab)DepositorInsertU6-sgRNA, EFS-PIN-dCas9
UseCRISPR and LentiviralAvailable SinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23 WT 2014 EBOV Delta-Mucin-Like-Domain
Plasmid#86021PurposeEbola Virus (Makona) Glycoprotein in CMV driven mammalian expression vectorDepositorInsertMakona Ebola Virus Glycoprotein
ExpressionMammalianMutationLacks mucin-like domainPromoterCMV IEAvailable SinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
61426-hU6-shmFmr1-EF1a-mCherry
Plasmid#157859PurposeExpresses a shRNA targeting mouse Fmr1 geneDepositorAvailable SinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRRLSIN.cPPT.PGK.MD(ND2bHLH)WCS.WPRE
Plasmid#122056PurposeLentiviral expression of a MyoD chimeric protein substituted with the NeuroD2 bHLH domain and point mutations to prevent MyoD interaction with PBX/MEIS: W96A, C98A, and S253PDepositorUseLentiviralExpressionMammalianMutationMyoD chimeric protein substituted with the NeuroD…Available SinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only