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pCA11-pMa-sgTS3-17xMS2V5-sgTS3

Plasmid

#199442

Purpose

CRISPR donor plasmid to insert 17XMS2V5 into the ribosomal DNA region in dependent of homology arms

Depositor

Baohui Chen

Article

Fu et al J Cell Biol. 2023 Jan 2;222(1):e20220211

Insert

HITI donor template

Use

CRISPR

Available Since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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pEJS2191_Nme2-evoFERNY-nt

Plasmid

#201527

Purpose

Mammalian expression of N-terminally fused Nme2-evoFERNY-nt

Depositor

Erik Sontheimer

Article

Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

Insert

Nme2-evoFERNY-nt

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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HIR K1030R

Plasmid

#24050

Depositor

Frederick Stanley

Article

Jacob et al Mol Cell Endocrinol. 2002 Jan 15. 186(1)

Insert

Insulin Receptor (INSR Human)

Expression

Mammalian

Mutation

K1030R

Available Since

Dec. 16, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-cyto-KB1691-Nluc

Plasmid

#204326

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

cytosolic KB1691-Nluc

Expression

Mammalian

Available Since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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SlutrCas9

Plasmid

#163795

Purpose

Expresses SlutrCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

Type

Empty backbone

Use

AAV and CRISPR

Expression

Mammalian

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pRK1035

Plasmid

#8832

Depositor

David Waugh

Article

Nallamsetty et al Protein Expr Purif. 2004 Nov . 38(1):108

Insert

TVMV protease

Tags

His and MBP (cleaved)

Expression

Bacterial

Mutation

synthetic gene with codons optimized for E. coli

Available Since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

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pLG021 (AVAST type 5)

Plasmid

#157899

Purpose

Expresses the AVAST type 5 defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

AVAST type 5 (STAND ATPase with N-terminal SIR2)

Expression

Bacterial

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lsh_C2c2_WT

Plasmid

#83487

Purpose

Bacterial expression of WT L. shahii C2c2 CRISPR effector

Depositor

Jennifer Doudna

Article

East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

Insert

Lsh_C2c2_WT

Use

CRISPR

Tags

His6-MBP

Expression

Bacterial

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Venus-StagRFP-AktAR

Plasmid

#173020

Purpose

Yellow-red FRET-based Akt/PKB activity reporter for multiplexed imaging applications.

Depositor

Jin Zhang

Article

Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

Insert

Venus-StagRFP-AktAR

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pT3TS-CD44b-TurboID

Plasmid

#163850

Purpose

In vitro transcription of CD44b-TurboID. Parton lab clone JTJ

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

CD44b (cd44b Zebrafish)

Use

In vitro transcription of rna

Tags

TurboID

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-N-I-N-CAAX

Plasmid

#41686

Purpose

Expresses caged Cdc42 GEF intersectin: Intersectin Dbl homology (DH) domain fused to Dronpa145N at C terminus and at N terminus, for optogenetic activation

Depositor

Michael Lin

Article

Zhou et al Science. 2012 Nov 9;338(6108):810-4. doi

Insert

Dronpa145N-IntersectinDH-Dronpa145N-CAAX

Tags

CAAX box

Expression

Mammalian

Mutation

K145N on Dronpa

Promoter

CMV

Available Since

Dec. 21, 2012

Availability

Academic Institutions and Nonprofits only

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pCAGGs-Foxp1

Plasmid

#45720

Depositor

Jeremy Dasen

Article

Dasen et al Cell. 2008 Jul 25;134(2):304-16. doi: 10

Insert

FoxP1 (Foxp1 Mouse)

Promoter

CMV / b-actin

Available Since

Aug. 13, 2013

Availability

Academic Institutions and Nonprofits only
pLentiCRISPR V2 sgIREB2_2

Plasmid

#169886

Purpose

Disrupt IREB2

Depositor

Richard Possemato

Article

Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

Insert

sgRNA targeting IREB2

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSynapsin1-GCaMP6s-P2A-mKate2

Plasmid

#112006

Purpose

For co-expression of GCaMP6s with mKate2

Depositor

Lin Tian

Article

Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

Has Service

AAV9

Insert

GCaMP6s-P2A-mKate2

Use

AAV

Expression

Mammalian

Promoter

hSynapsin1

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pGEMTEZ-Kir2.1

Plasmid

#32641

Depositor

Richard Axel, Joseph Gogos, C. Ron Yu

Article

Yu et al Neuron. 2004 May 27;42(4):553-66.

Insert

Kir2.1 (Kcnj2 Mouse)

Expression

Bacterial

Available Since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only

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pBT137-splitD

Plasmid

#122606

Purpose

clone M13 phage by SapI Golden Gate

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

M13 bacteriophage genes

Expression

Bacterial

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-DIO-GRAB_ATP1.0

Plasmid

#167581

Purpose

Express the ATP sensor GRAB_ATP1.0 in Cre positive cells

Depositor

Yulong Li

Article

Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

Insert

ATP sensor GRAB_ATP1.0 (P2RY1 Human)

Use

AAV

Promoter

EFS

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-hSyn-Galpha-i1 ONE-GO

Plasmid

#204333

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

hSyn Galpha-i1 ONE-GO

Use

Lentiviral

Available Since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLG017 (AVAST type 1)

Plasmid

#157895

Purpose

Expresses the AVAST type 1 defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

AVAST type 1 (STAND ATPase with N-terminal serine protease)

Expression

Bacterial

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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GFAP-Arclight

Plasmid

#187978

Purpose

Expresses the fluorescent voltage indicator Arclight under the GFAP promoter

Depositor

Chris Dulla

Article

Armbruster et al Nat Neurosci. 2022 May;25(5):607-616. do

Insert

Arclight A242

Use

AAV

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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