We narrowed to 53,425 results for: plasmid

1. pLP-hSox2-ires-gfp

   Plasmid

   #49391

   Purpose

   mammalian bicistronic expression of human Sox2 and EGFP

   Depositor

   Randall McKinnon

   Insert

   Sox2 (SOX2 Human)
   

   Use

   Cre/Lox; Acceptor vector

   Tags

   6xHN, EGFP, and IRES

   Expression

   Mammalian

   Mutation

   TGA (stop) to CCA (Pro) at nt 1027 with respect t…

   Promoter

   CMV

   Available since

   Dec. 2, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pGBW-m4134904

   Plasmid

   #151930

   Purpose

   Mammalian expression plasmid for SARS-CoV-2 spike prefusion-stabilized

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 spike prefusion-stabilized (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   d1-15 d1209-1273 R682A R685G K986P V987P

   Promoter

   CMV

   Available since

   May 20, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pGBW-m4046299

   Plasmid

   #153878

   Purpose

   Yeast expression plasmid for SARS-CoV-2 S (surface glycoprotein)

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable thrombin;6xHIS

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pGAL1

   Available since

   June 12, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. Syn-ChR-tdTomato

   Plasmid

   #99843

   Purpose

   AAV expression of ChR2(H134R)-tdTomato under the Syn promoter

   Depositor

   Gordon Shepherd

   Insert

   ChR2(H134R)-tdTomato
   

   Use

   AAV

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   ChR2(H134R), carrying a single point mutation at …

   Promoter

   human synapsin I promoter

   Available since

   Jan. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGBW-m4134207

   Plasmid

   #152431

   Purpose

   Mammalian expression plasmid for SARS-CoV-2 S (surface glycoprotein)

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;3xFlag

   Expression

   Mammalian

   Mutation

   wt

   Promoter

   CMV

   Available since

   July 30, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pGBW-m4133141

   Plasmid

   #152404

   Purpose

   Mammalian expression plasmid for SARS-CoV-2 S (surface glycoprotein)

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;3xFlag

   Expression

   Mammalian

   Mutation

   wt

   Promoter

   CMV

   Available since

   June 17, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pGBW-m4258326

   Plasmid

   #154793

   Purpose

   Mammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 surface glycoprotein (Spike protein) (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;V5

   Expression

   Mammalian

   Mutation

   wt

   Promoter

   CMV

   Available since

   July 30, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pGBW-m4133806

   Plasmid

   #153713

   Purpose

   Mammalian expression plasmid for SARS-CoV-2 S (surface glycoprotein)

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;S-tag

   Expression

   Mammalian

   Mutation

   wt

   Promoter

   CMV

   Available since

   June 12, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. WDR5

   Plasmid

   #36900

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   WDR5 (WDR5 Human)
   

   Use

   Tags

   His tag with TEV cleavage

   Expression

   Bacterial

   Mutation

   mutation version of plasmid #60121 3UR4

   Promoter

   Available since

   Aug. 8, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

10. pGBW-m4252903

    Plasmid

    #153778

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 E (envelope)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 E (envelope) (E Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;V5

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pGBW-m4046457

    Plasmid

    #145424

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 M (membrane)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pTEF1

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pGBW-m4046390

    Plasmid

    #148996

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp3

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp3 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Use

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pTEF1

    Available since

    May 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pGBW-m4046332

    Plasmid

    #145413

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 3C-like proteinase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Use

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pJUNB-donor

    Plasmid

    #112313

    Purpose

    CRISPR donor plasmid to tag human transcription factor JUNB with GFP

    Depositor

    Kevin White

    Insert

    JUNB homology arms flanking EGFP-IRES-Neo cassette (JUNB Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    hg38:19:12792992:C>G, hg38:19:12793018:T>G,…

    Promoter

    Available since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGBW-m4046207

    Plasmid

    #145559

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp6

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp6 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Use

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pGBW-m4046418

    Plasmid

    #149029

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 N (nucleocapsid)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 N (nucleocapsid) (N Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Use

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pGAL1

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pYCTK025

    Plasmid

    #176729

    Purpose

    The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Ste2 gene of Lachancea mirantina (receiver part).

    Depositor

    Victor Sourjik

    Article

    Krink et al bioRxiv 2021

    Insert

    ste2Lm
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Codon-optimized for expression in S. cerevisiae

    Promoter

    Available since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pYCTK028

    Plasmid

    #176732

    Purpose

    The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Ste2 gene of Tetrapisispora phaffii (receiver part).

    Depositor

    Victor Sourjik

    Article

    Krink et al bioRxiv 2021

    Insert

    ste2Tp
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Codon-optimized for expression in S. cerevisiae

    Promoter

    Available since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGBW-m4046640

    Plasmid

    #153906

    Purpose

    Yeast expression plasmid for SARS-CoV-2 S (surface glycoprotein)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pGL3-basic-hFX-promotor(2,5kb)

    Plasmid

    #14980

    Depositor

    Michael Ristow

    Insert

    human frataxin promoter fragment (FXN Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    start codon for human frataxin gene has been muta…

    Promoter

    Available since

    Aug. 17, 2007

    Availability

    Academic Institutions and Nonprofits only