164,223 results

1. pLEM415-ldhL-mRFP1

   Plasmid

   #99842

   Purpose

   Expression of RFP in Lactobacillus

   Depositor

   Sujin Bao

   Article

   Bao et al PLoS One. 2013;8(3):e60007. doi: 10.1371

   Insert

   monomeric red fluorescent protein
   

   Use

   Tags

   FLAG

   Expression

   Bacterial

   Mutation

   Promoter

   ldhl

   Available Since

   Nov. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. p6sheLLr

   Plasmid

   #37318

   Depositor

   John Schiller

   Article

   Pastrana et al Virology. 2005 Jul 5;337(2):365-72.

   Insert

   HPV6L1+L2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMpGWB103

   Plasmid

   #68557

   Purpose

   Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

   Depositor

   Takayuki Kohchi

   Article

   Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   MpEF1alpha promoter

   Available Since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMpGWB326

   Plasmid

   #68654

   Purpose

   Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

   Depositor

   Takayuki Kohchi

   Article

   Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

   Type

   Empty backbone

   Use

   Tags

   TagRFP

   Expression

   Plant

   Mutation

   Promoter

   No Promoter

   Available Since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLVX-opto-mMLKL

   Plasmid

   #208784

   Purpose

   Inducibly expresses mouse MLKL fused to light-activatable Cry2

   Depositor

   Petr Broz

   Article

   Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

   Insert

   mCherry mMLKL Cry2 (Mlkl Mouse)
   

   Use

   Lentiviral

   Tags

   Cry2 and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLJM60-Ptb

   Plasmid

   #38245

   Depositor

   David Sabatini

   Article

   Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

   Insert

   Ptb (Ptbp1 Mouse)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 7, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGWB501

   Plasmid

   #74843

   Purpose

   Gateway cloning compatible binary vector for simple cloning of gene.

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   No Promoter

   Available Since

   Aug. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pnCS_SEPT6_SEPT7-TEV-Strep

   Plasmid

   #174499

   Purpose

   bacterial co-expression of human SEPT6 and of human SEPT7

   Depositor

   Manos Mavrakis

   Article

   Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

   Inserts

   SEPTIN6 (SEPTIN6 Human)
   SEPTIN7 (SEPTIN7 Human)
   

   Use

   Tags

   TEV-Strep

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-hDAT

   Plasmid

   #32810

   Depositor

   Susan Amara

   Insert

   DAT (SLC6A3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJMP1159

    Plasmid

    #119250

    Purpose

    rfp "test" strain

    Depositor

    Jason Peters, Oren Rosenberg, Carol Gross

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    sgRNA NT1/RR1 (rfp)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCk4_spacer

    Plasmid

    #136074

    Purpose

    Use when making even level constructs (L2,...) if pCk4 position (position 4) has no construct assigned

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    Odd spacer
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available Since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pRS41K-mCherry-sfGFP (pJJF001)

    Plasmid

    #173457

    Purpose

    C-SWAT donor for Seamless tagging, contains mCherry-sfGFP tandem fluorescent protein timer

    Depositor

    Anton Khmelinskii

    Article

    Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

    Insert

    mCherry-sfGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    no promoter

    Available Since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA-D3cpv

    Plasmid

    #36323

    Depositor

    Roger Tsien, Amy Palmer

    Article

    Palmer et al Chem Biol. 2006 May;13(5):521-30.

    Insert

    D3cpv second generation cameleon (calcium sensor)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDH-EF1-Large BiT - SNAP-tag - Beta-actin

    Plasmid

    #171019

    Purpose

    Lentiviral expression of a Large BiT-SNAP-tag-Beta-actin fusion in mammalian cells

    Depositor

    Angus Johnston

    Article

    Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

    Insert

    LargeBit-SNAPtag- Beta-actin
    

    Use

    Lentiviral and Luciferase

    Tags

    Beta actin and LargeBiT

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. L1_lacZgRNA-Ck3

    Plasmid

    #136136

    Purpose

    L1 in position 3, to clone gRNA target using BbsI, lacZ blue-white screening

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    p5-MpU6:lacZgRNA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. HMGA2(3'UTR DEL)+GFP

    Plasmid

    #25406

    Depositor

    Sean Morrison

    Article

    Nishino et al Cell. 2008 Oct 17. 135(2):227-39.

    Insert

    high mobility group AT-Hook 2 (Hmga2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    3" UTR is absent

    Promoter

    Available Since

    July 14, 2010

    Availability

    Academic Institutions and Nonprofits only

17. pCMV-hAR

    Plasmid

    #89078

    Purpose

    mammalian expression of human androgen receptor in pCMV5

    Depositor

    Elizabeth Wilson

    Article

    Lubahn et al Science. 1988 Apr 15;240(4850):327-30.

    Insert

    human androgen receptor (hAR) full-length (AR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA-K-GECO1

    Plasmid

    #105865

    Purpose

    Mammalian cell expression plasmid for red fluorescent Ca2+ indicator K-GECO1

    Depositor

    Robert Campbell

    Article

    Shen et al BMC Biol. 2018 Jan 16;16(1):9. doi: 10.1

    Insert

    K-GECO1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEVOL-pylT-N346A/C348A

    Plasmid

    #127411

    Purpose

    Double mutant of wild type mmPylRS designed for incorporation of non-canonical amino acids with mono-substituted phenylalanine derivatives and tyrosinyl ethers

    Depositor

    Wenshe Liu

    Article

    Wang et al J Am Chem Soc. 2012 Feb 15;134(6):2950-3

    Insert

    Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N346A-C348A

    Promoter

    araBAD

    Available Since

    July 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. L1_Cas9-Ck4

    Plasmid

    #136135

    Purpose

    L1 in position 4, for AtcoCas9 (Puchta) expression in L2 CRISPR vectors

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    p5-MpEF1a:Cas9_3tNos-35S
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. TFORF3023

    Plasmid

    #144499

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NEUROD6 (NEUROD6 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Sept. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. HMGA2 shRNA

    Plasmid

    #25408

    Depositor

    Sean Morrison

    Article

    Nishino et al Cell. 2008 Oct 17. 135(2):227-39.

    Insert

    high mobility group AT-Hook 2 (Hmga2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available Since

    July 14, 2010

    Availability

    Academic Institutions and Nonprofits only

23. pDONR221-Hyg

    Plasmid

    #48994

    Purpose

    Gateway adapted transfer of Hygromycin resistance gene

    Depositor

    Rick Tarleton

    Article

    Xu et al BMC Microbiol. 2009 May 11;9:90. doi: 10

    Insert

    hygromycin B phosphotransferase
    

    Use

    Gateway attb1 & attb2

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. pCdc45-mCherry

    Plasmid

    #211380

    Purpose

    mammalian expression of human Cdc45 C-terminally fused to mCherry

    Depositor

    Helmut Pospiech

    Insert

    Cdc45 (CDC45 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCRISPathBrick

    Plasmid

    #65006

    Purpose

    E. coli vector for expression of S. pyogenes dCas9, tracrRNA, and nontargeting CRISPR array with BsaI site for inserting user-defined spacer-repeat bricks

    Depositor

    Mattheos Koffas

    Article

    Cress et al ACS Synth Biol. 2015 Mar 30.

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Constitutive native promoters

    Available Since

    May 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. plVX-EF1a-FLAG-hMETTL3-IRES-ZsGreen

    Plasmid

    #207123

    Purpose

    Lentiviral vector to express human METTL3

    Depositor

    Silvia Monticelli

    Article

    Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

    Insert

    hMETTL3 (METTL3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Hygro-Cas9 donor

    Plasmid

    #86883

    Purpose

    Donor vector for genomic targeting of a Tetracycline-inducible Cas9 cassette to the human AAVS1/PPP1R12C locus with hygromycin selection

    Depositor

    Danwei Huangfu

    Article

    Shi et al Cell Stem Cell. 2017 Feb 8. pii: S1934-5

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    3xFlag, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. GAL4BD-VP16/pDON207

    Plasmid

    #201230

    Purpose

    GAL4-VP16 transcription factor

    Depositor

    Michael Ayliffe

    Article

    Chen et al Plant J. 2023 Jun;114(6):1209-1226. doi:

    Insert

    GAL4BD-VP16
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    July 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. EB3-GFP

    Plasmid

    #190164

    Purpose

    Expresses EB3-GFP in mammalian cells

    Depositor

    Anna Akhmanova

    Article

    Stepanova et al J Neurosci. 2003 Apr 1;23(7):2655-64.

    Insert

    EB3-GFP
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Lenti-dCas9-TurboID-blast

    Plasmid

    #222599

    Purpose

    Express fussed dCas9-TurboID for proximity labeling

    Depositor

    Yarui Diao

    Article

    Sun et al Nat Genet. 2023 Aug;55(8):1324-1335. doi

    Insert

    dCas9-TurboID
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF-1α

    Available Since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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