Addgene

We narrowed to 17,703 results for: STO

Showing: 8611 - 8640 of 17703 results

Results per page:

Sort results by:

pET21-10XHis-GST-HRV-Her2-dL5-Her2

Plasmid

#85624

Purpose

Bacterial expression vector for Z342-dL5(E52D)-Z342 affibody fusion protein (AFA) with N-terminal His10, GST and HRV3C cleavage site (MBIC5, dL5**, FAP, AffiFAP)

Depositor

Marcel Bruchez

Insert

10XHis-GST-HRV-Her2-dL5-Her2 (EGFR Schistosoma japonicum, Synthetic, Human)

Use

Tags

10XHis-GST-HRV and The Her2 Affibody and dL5 FAP …

Expression

Bacterial

Mutation

The dL5** FAP is E50D, L89S, also known as E52D/L…

Promoter

T7

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLEX307-SARS-CoV-3CL-C145A

Plasmid

#160281

Purpose

Allows for constituitive mammalian expression of the SARS-CoV 3CL protease with C145A mutation via transfection or lentivirus

Depositor

Alejandro Chavez

Article

Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

Insert

3CL Protease

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

C145A

Promoter

EF1a

Available Since

Oct. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSR:MSMEG_0028:Dendra

Plasmid

#159038

Purpose

T7 expression of C-term fluorescent fusion in E. coli

Depositor

Keith Derbyshire, Joseph Wade

Article

Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

Insert

MSMEG_0028

Use

Integrating mycobacterial expression vector attl5

Tags

Gly-Ala Linker/Dendra2/1x Flag Tag

Expression

Bacterial

Mutation

Promoter

pmycTetO

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBabepuro3-p18 F37I

Plasmid

#24936

Depositor

Lynda Chin

Article

Wiedemeyer et al Cancer Cell. 2008 Apr . 13(4):355-64.

Insert

p18 (CDKN2C Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

F37I

Promoter

Available Since

June 23, 2010

Availability

Academic Institutions and Nonprofits only
PB-hSyn-mSyp-RT1-24xGCN4

Plasmid

#106300

Purpose

The plasmid encodes mouse Synaptophysin sparsely labeled with SunTag24x_v4.

Depositor

James Zhe Liu

Article

Liu et al Proc Natl Acad Sci U S A. 2017 Dec 28. p

Insert

mSyp-RT1-24xGCN4 (Syp Mouse)

Use

Tags

24xGCN4

Expression

Mammalian

Mutation

Promoter

human Synapsin1

Available Since

March 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry-hPMCA2xb

Plasmid

#47751

Purpose

mammalian expression of mCherry tagged hPMCA2, xb splice variant

Depositor

Emanuel Strehler

Article

Paszty et al Sci Signal. 2015 Feb 17;8(364):ra19. doi

Insert

PMCA2x/b (ATP2B2 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

xb splice variant

Promoter

CMV

Available Since

Nov. 23, 2018

Availability

Academic Institutions and Nonprofits only
pSG5-FLAG-mEKLF

Plasmid

#67833

Purpose

Expression of FL-EKLF driven by SV40 promoter

Depositor

James Bieker

Article

Soni et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

Insert

EKLF (Klf1 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Full-length mEKLF is aa 20-376; amino acid 19 is …

Promoter

SV40

Available Since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only
OCT4 GFP puro Donor 3

Plasmid

#22211

Depositor

Rudolf Jaenisch

Article

Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

Insert

SA-OCT-GFP-2APuro-PA (POU5F1 Human)

Use

Tags

Expression

Mutation

Promoter

Available Since

Oct. 5, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
His6tev CBP BCHZDeltaAL

Plasmid

#99551

Purpose

Bacterial expression of His tagged mouse CBP HAT Brd-ZZ(1079-1751) 1557-1617 replaced by SGGSG

Depositor

Peter Wright

Article

Park et al Proc Natl Acad Sci U S A. 2017 Jun 19. p

Insert

mouse CBP BRD-ZZ domains (1079-1751) DeltaAL (Crebbp Mouse)

Use

Tags

His6tev

Expression

Bacterial

Mutation

replaced 1557-1618 with SGGSG

Promoter

T7

Available Since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a_DIO_R133C-HaloTag

Plasmid

#164064

Purpose

Double-Floxed Inverted Open reading frame for mouseMeCP2(alpha) carrying R133C mutation

Depositor

Nathaniel Heintz

Article

Piccolo et al Elife. 2019 Dec 23;8. pii: 51449. doi: 1

Insert

MeCP2 Isoform 1 R133C (Mecp2 Mouse)

Use

AAV

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

EF1a

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLEX307-MERS-CoV-3CL-C148A

Plasmid

#160283

Purpose

Allows for constituitive mammalian expression of the MERS-CoV 3CL protease with C145A mutation via transfection or lentivirus

Depositor

Alejandro Chavez

Article

Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

Insert

3CL Protease

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

C148A

Promoter

EF1a

Available Since

Oct. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Cas9_YTHDF2_sgRNA

Plasmid

#186673

Purpose

YTHDF2 sgRNA plasmid

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

YTHDF2 KO sgRNA Plasmid (YTHDF2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiCRISPR v2-sgACSL4 clone3

Plasmid

#162130

Purpose

Lentiviral sgRNA plasmid targeting human ACSL4

Depositor

Boyi Gan

Article

Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

Insert

sgACSL4 (ACSL4 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
mCardinal-H3-23

Plasmid

#56163

Purpose

Localization: Nucleus/Histones, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

H3 (H3C2 Human)

Use

Tags

mCardinal

Expression

Mammalian

Mutation

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pMSR:MSMEG_0132:Dendra

Plasmid

#159002

Purpose

T7 expression of C-term fluorescent fusion in E. coli

Depositor

Keith Derbyshire, Joseph Wade

Article

Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

Insert

MSMEG_0132

Use

Integrating mycobacterial expression vector attl5

Tags

Gly-Ala Linker/Dendra2/1x Flag Tag

Expression

Bacterial

Mutation

Promoter

pmycTetO

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1puro-shKIBRA-A

Plasmid

#40888

Depositor

Paul Reynolds

Article

Moleirinho et al Oncogene. 2012 May 21. doi: 10.1038/onc.

Insert

shKIBRA-A (WWC1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available Since

March 7, 2013

Availability

Academic Institutions and Nonprofits only
pRP[2CRISPR]-mCherry/Hygro-hCas9-U6>{mdx left}-U6>{mdx right}

Plasmid

#184381

Purpose

This plasmid contains hCas9 and two gRNAs that can excise the genomic area around the mouse mdx mutation in dystrophin's exon 23

Depositor

Ori Bar-Nur

Article

Domenig et al Stem Cell Reports. 2022 Feb 8;17(2):321-

Insert

Cas9, two gRNAs targeting dystrophin

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1puro-shKIBRA-B

Plasmid

#40889

Depositor

Paul Reynolds

Article

Moleirinho et al Oncogene. 2012 May 21. doi: 10.1038/onc.

Insert

shKIBRA-B (WWC1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available Since

Nov. 5, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pLEX307-IBV-3CL-C143A

Plasmid

#160289

Purpose

Allows for constituitive mammalian expression of the IBV 3CL protease with C143A mutation via transfection or lentivirus

Depositor

Alejandro Chavez

Article

Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

Insert

3CL Protease

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

C143A

Promoter

EF1a

Available Since

Oct. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG71 BDNF 3'UTR mut

Plasmid

#12054

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

BDNF 3'UTR mut (BDNF Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Mutations in microRNA recognition sites (seed mat…

Promoter

Available Since

June 2, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-C1 hSlp2-a del.SHD

Plasmid

#40050

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp2-a del.SHD (SYTL2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

deletion of SHD domain (1-56)

Promoter

CMV promoter

Available Since

Nov. 8, 2012

Availability

Academic Institutions and Nonprofits only
AOI-WT-Cas9-sg-mouse Suz12-F2-GFP

Plasmid

#91882

Purpose

Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Suz12

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

gRNA targeting Suz12 (Suz12 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only
pGEX-2T-TRR-C421-Y2404F

Plasmid

#182846

Purpose

mutation Y2404F (TAC/TTC) in GST-TRR-C421 (TRR 2011-2431 aa). Expression in E. coli. by IPTG induction

Depositor

Peter Harte

Article

Tie et al Development. 2014 Mar;141(5):1129-39. do

Insert

TRR 2011-2431 (trr Fly)

Use

Tags

Expression

Bacterial

Mutation

mutation Y2404F (TAC/TTC) in GST-TRR-C421 (TRR 20…

Promoter

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
DSPQ-HTT-TALEN2 BSD

Plasmid

#92246

Purpose

TALEN targeting downstream of HTT gene (ELD) with Blasticidin selection, forms obligate heterodimer with DSPQ-HTT-TALEN1 ZEO. TALEN: NN, HD, NN, NN, HD, NG, NN, NI, NN, NN, HD, NI, NN, HD, NI, NN

Depositor

Mahmoud Pouladi

Article

Ooi et al Cell Rep. 2019 Feb 26;26(9):2494-2508.e7

Insert

TALEN

Use

TALEN

Tags

Expression

Mutation

Promoter

CAG

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
DSPQ-HTT-TALEN1 ZEO

Plasmid

#92245

Purpose

TALEN targeting downstream of HTT gene (KKR) with Blasticidin selection, forms obligate heterodimer with DSPQ-HTT-TALEN2 BSD. TALEN: HD, NI, NN, HD, NG, NG, HD, HD, NG, HD, NI, NN, HD, HD, NN, HD

Depositor

Mahmoud Pouladi

Article

Ooi et al Cell Rep. 2019 Feb 26;26(9):2494-2508.e7

Insert

TALEN

Use

TALEN

Tags

Expression

Mutation

Promoter

CAG

Available Since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSR:MSMEG_0319:Dendra

Plasmid

#159010

Purpose

T7 expression of C-term fluorescent fusion in E. coli

Depositor

Keith Derbyshire, Joseph Wade

Article

Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

Insert

MSMEG_0319

Use

Integrating mycobacterial expression vector attl5

Tags

Gly-Ala Linker/Dendra2/1x Flag Tag

Expression

Bacterial

Mutation

Promoter

pmycTetO

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSR:MSMEG_0311:Dendra

Plasmid

#159052

Purpose

T7 expression of C-term fluorescent fusion in E. coli

Depositor

Keith Derbyshire, Joseph Wade

Article

Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

Insert

MSMEG_0311

Use

Integrating mycobacterial expression vector attl5

Tags

Gly-Ala Linker/Dendra2/1x Flag Tag

Expression

Bacterial

Mutation

Promoter

pmycTetO

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSS02:cyt-Peredox-mCherry

Plasmid

#161743

Purpose

cytosolic expression of fluorescent NADH/NAD+ biosensor Peredox-mCherry in plants

Depositor

Markus Schwarzländer

Article

Steinbeck et al Plant Cell. 2020 Aug 13. pii: tpc.20.002

Insert

cyt-Peredox-mCherry

Use

Tags

Expression

Plant

Mutation

Promoter

Ubiquitin 10

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST47-ARF4-GFP

Plasmid

#67392

Purpose

Mammalian expression of hArf4 with C-term GFP tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF4 (ARF4 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

V53A, L123P and M134I mutations were unintended P…

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pMSR:MSMEG_1346:Dendra

Plasmid

#174997

Purpose

C-term fluorescent fusion

Depositor

Keith Derbyshire, Joseph Wade

Article

Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

Insert

MSMEG_1346

Use

Integrating mycobacterial expression vector attl5…

Tags

Gly-Ala Linker/Dendra2/1x Flag Tag

Expression

Bacterial

Mutation

Promoter

pmycTetO

Available Since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge