Addgene

We narrowed to 21,422 results for: ESC;

Showing: 8641 - 8670 of 21422 results

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pcDNA3-K-I-N-CAAX

Plasmid

#41640

Purpose

Expresses caged Cdc42 GEF intersectin: Intersectin Dbl homology (DH) domain fused to wild-type Dronpa145K at C terminus and Dronpa145N at N terminus, for optogenetic activation

Depositor

Michael Lin

Article

Zhou et al Science. 2012 Nov 9;338(6108):810-4. doi

Insert

Dronpa145K-IntersectinDH-Dronpa145N-CAAX

Use

Tags

CAAX box

Expression

Mammalian

Mutation

K145N on Dronpa

Promoter

CMV

Available since

Dec. 21, 2012

Availability

Academic Institutions and Nonprofits only

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pGEX-HA-GlpG

Plasmid

#155135

Purpose

Expresses N-terminally GFP- and HA-tagged E. coli GlpG from pGEX 4P-1

Depositor

Sinisa Urban

Article

Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910

Insert

GlpG (glpG Escherichia coli)

Use

Tags

GST and HA

Expression

Bacterial

Mutation

Promoter

tac

Available since

Sept. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2+_Flag-myc-NES-Tir1

Plasmid

#117717

Purpose

mRNA synthesis of Flag-myc-NES-TIR1 for injection

Depositor

Joerg Mansfeld

Article

Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

Insert

TRANSPORT INHIBITOR RESPONSE 1

Use

Tags

Flag-Myc-NES

Expression

Mammalian

Mutation

codon optimized TIR1 for murine expression

Promoter

SP6

Available since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pINDUCER20-Flag-hSPDEF WT

Plasmid

#100873

Purpose

Lentiviral expression of human SPDEF

Depositor

Noah Shroyer

Article

Lo et al Gastroenterology. 2017 Jul;153(1):205-21

Insert

SPDEF (SPDEF Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

TRE2

Available since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-tagRFP-NLS-HA-Bxb1

Plasmid

#65625

Purpose

Vector for cotranslational expression of tagRFP-Bxb1

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

Bxb1 integrase

Use

Tags

HA, SV40 NLS, and tagRFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 31, 2015

Availability

Academic Institutions and Nonprofits only

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pAJM.969

Plasmid

#108537

Purpose

MphRAM + EryR + PMph-YFP Reporter. Ery sensor for use in non-Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

MphRAM + EryR + PMph-YFP

Use

Tags

Expression

Bacterial

Mutation

MphR: N94D

Promoter

Available since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pACUH-sfcherry2(1-10)

Plasmid

#172061

Purpose

UAS vector to express sfcherry2(1-10) in Drosophila

Depositor

Daichi Kamiyama

Article

Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

Insert

sfcherry2(1-10)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pLP-mScarlet-I

Plasmid

#209993

Purpose

Contains Level 0 Part: Coding sequences (mScarlet-I) for the construction of Level 1 plasmids

Depositor

Georg Fritz, Thorsten Mascher

Article

Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

Insert

mScarlet-I

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pLifeAct_mScarlet-H_N1

Plasmid

#85055

Purpose

In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

LifeAct

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_Beta4Gal-HaloTag7-T2A-Lyn11-SNAPf

Plasmid

#175524

Purpose

Co-expression of HaloTag7 (gogli) and SNAPf-tag (membrane inner leaflet) in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Beta4Gal-HaloTag7 and Lyn11-SNAPf

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE-TO-Sp dCas9-mVenusN

Plasmid

#191396

Purpose

SpdCas9 fused to N-terminal part of mVenus

Depositor

Albert Jeltsch

Article

Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

Insert

N-terminal part of split mVenus

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pJWV25

Plasmid

#61045

Purpose

To construct N-terminal GFP fusions under control of a Zn2+ inducible promoter, integrates via double crossover at bgaA

Depositor

Jan-Willem Veening

Article

Eberhardt et al Mol Microbiol. 2009 Oct;74(2):395-408. d

Type

Empty backbone

Use

Synthetic Biology

Tags

GFP+

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pRSETb-ffDronpa

Plasmid

#78182

Purpose

Expresses ffDronpa E. coli strains

Depositor

Peter Dedecker

Article

Moeyaert et al ACS Nano. 2014 Feb 25;8(2):1664-73. doi:

Insert

ffDronpa

Use

Tags

6xHis-tag; Xpress tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 27, 2016

Availability

Academic Institutions and Nonprofits only

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IFP2.0-C1

Plasmid

#54784

Purpose

Localization: C1 Cloning Vector, Excitation: 684, Emission: 708

Depositor

Xiaokun Shu, Michael Davidson

Article

Yu et al Nat Commun. 2014 May 15;5:3626. doi: 10.

Type

Empty backbone

Use

Tags

IFP2.0

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.nucGAP

Plasmid

#78736

Purpose

Expression of Ca2+ sensor GAP in the nucleus

Depositor

Teresa Alonso

Article

Rodriguez-Garcia et al Proc Natl Acad Sci U S A. 2014 Feb 18;11

Insert

nucGAP

Use

Tags

nucleoplasmin (Xenopus)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 21, 2016

Availability

Academic Institutions and Nonprofits only
pX330-Flag-wtSpCas9-H840A

Plasmid

#80453

Purpose

SpCas9 mammalian expression vector

Depositor

Ervin Welker

Article

Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

Insert

SpCas9_H840A

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

Promoter

CBh

Available since

Aug. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pCVL.SFFV.Kozak.HA.NLS.SceOpt.IRES.BFP

Plasmid

#45574

Purpose

Expresses I-Sce I nuclease in mammalian cells with BFP tracker in lentiviral backbone

Depositor

Andrew Scharenberg

Article

Kuhar et al Nucleic Acids Res. 2013 Oct 10.

Insert

co I-Sce I

Use

Lentiviral

Tags

HA, IRES BFP, and NLS

Expression

Mammalian

Mutation

co indicates codon optimization for mammalian exp…

Promoter

SFFV

Available since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag-MBD1-14-mVenusC

Plasmid

#191393

Purpose

MBD domain of MBD1 fused to C-terminal part of split mVenus

Depositor

Albert Jeltsch

Article

Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

Insert

pcDNA3 Flag-MBD1-14-mVenusC

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-3xhfYFP

Plasmid

#186544

Purpose

Hyperfolder YFP, triple fusion: cytosolic expression in mammalian cells

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

Hyperfolder YFP (3X)

Use

Tags

Expression

Mammalian

Mutation

mGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…

Promoter

CMV

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pM2s2AsR_TS-E10

Plasmid

#137944

Purpose

E. coli Nissle pMUT2-derived plasmid with ampicillin resistance and constitutive RFP expression and strong temperature sensitive GFP expression

Depositor

Neel Joshi

Article

Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102

Inserts

mRFP1
mWasabi

Use

Synthetic Biology; For use with e. coli nissle in…

Tags

Expression

Bacterial

Mutation

Promoter

J23101 and pTlpA-C7

Available since

May 3, 2021

Availability

Academic Institutions and Nonprofits only

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pPM1001

Plasmid

#167548

Purpose

Encodes genes that confer expression of Vibrio cholerae O antigens of the Inaba serotype in E. coli K-12

Depositor

Renato Morona

Article

Manning et al Infect Immun. 1986 Aug;53(2):272-7. doi:

Insert

Vibrio cholerae O antigens genes of the Inaba serotype

Use

Cosmid

Tags

Expression

Mutation

Promoter

Available since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pCytERM-Electra1

Plasmid

#179476

Purpose

ER Visualization (OSER assay) Expresses Electra1 in fusion with N-terminal part of p450

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

CytERM-Electra1

Use

Tags

Electra1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_flGalT-SBP-mCherry

Plasmid

#65273

Purpose

synchronize trafficking of GalT from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

galactosidase

Use

Tags

Streptavidin Binding Protein (SBP) and mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 10, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1X-Nup88

Plasmid

#87338

Purpose

To express human Nup88 in mammalian cells

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

NUP88 (NUP88 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pAC-4CL1

Plasmid

#35947

Depositor

Claudia Schmidt-dannert

Article

Watts et al BMC Biotechnol. 2006 Mar 21;6:22.

Insert

4-coumaroyl CoA ligase 1 (4CL1 Mustard Weed)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pLac-mod

Available since

Sept. 5, 2012

Availability

Academic Institutions and Nonprofits only

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pBEST-p15A-Pl-tetO1-deGFP-T500 (122p)

Plasmid

#70198

Purpose

Promoter panel

Depositor

Richard Murray

Article

Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

Insert

deGFP

Use

Tags

Expression

Mutation

Promoter

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pCNA

Plasmid

#135191

Purpose

Arabinose inducible expression of lamba-Red recombinase proteins and I-CreI.

Depositor

Hongqing Fang

Article

Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d

Insert

I-CreI lamba Red

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

None

Promoter

araBAD

Available since

March 5, 2020

Availability

Academic Institutions and Nonprofits only

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pLSU4cg10

Plasmid

#126078

Purpose

Plant transformation vector for stable expression of plastid targeted GFP1-10 in plant cells

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

Chloroplastic FNR transit peptide

Use

Binary

Tags

GFP1-10

Expression

Plant

Mutation

Promoter

longCaMV35S

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pET15b T7pCONS sfGFP

Plasmid

#154467

Purpose

pET15b with a design flaw in the T7 promoter fixed. Contains the coding sequence for sfGFP

Depositor

Daniel Daley

Article

Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

Insert

super folder GFP

Use

Tags

His-thrombin

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRU53

Plasmid

#167679

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pEC1.2::spCas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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