We narrowed to 11,691 results for: NSI

1. pNS3_Ptrc::luxR _Plux::mNG

   Plasmid

   #189572

   Purpose

   Fluorescent reporter for Lux activation by 3OC6-HSL

   Depositor

   Daniel Ducat

   Article

   Kokarakis et al ACS Synth Biol. 2023 Jan 20;12(1):265-27

   Inserts

   Transcriptional activator luxR
   Monomeric Neon Green
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   lux and trc

   Available Since

   March 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. Kif3A-EGFP-SNAP

   Plasmid

   #229852

   Purpose

   Kif3A (aa1-342)-Kif1A neck linker(17aa) fused to Kin1 coiled coil (aa345-406)-EGFP-SNAP-his. This Kif3A was used to link an oligo to the motor using the SNAP tag for single molecule experiments

   Depositor

   William Hancock

   Insert

   Kif3A (Kif3a Mouse)
   

   Tags

   6xHis, EGFP, and SNAP-tag

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. E547* FL USP7

   Plasmid

   #229001

   Purpose

   E547-mutant of human USP7

   Depositor

   Irina Bezsonova

   Insert

   USP7 1-546 (USP7 Human)
   

   Tags

   6xHis cleavable with thrombin

   Expression

   Bacterial

   Mutation

   E547 mutated to stop codon

   Promoter

   T7

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLL3.7m-rsGreenF-E-Geminin(1-110)-IRES-Dronpa-Cdt(30-120)

   Plasmid

   #205761

   Purpose

   Single-color FUCCI4 sensor for G1/S cell cycle transition and M/G1 transition

   Depositor

   Edward Boyden

   Article

   Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

   Insert

   rsGreenF-E-Geminin(1-110)-IRES-Dronpa-Cdt(30-120)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   wt

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGG-PIP12

   Plasmid

   #196750

   Purpose

   Contains FLS2 promoter

   Depositor

   Marc Somssich

   Article

   Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

   Insert

   FLAGELLIN-SENSITIVE 2 (FLS2 Mustard Weed)
   

   Use

   Greengate (gg) entry vector

   Available Since

   Dec. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. hnRNPAB X2-RBD

   Plasmid

   #216516

   Purpose

   Expresses MBP-tagged RNA binding domain of hnRNPAB X2

   Depositor

   Nicolas Fawzi

   Article

   O'Connell et al Sci Rep. 2024 Nov 13;14(1):27890. doi: 1

   Insert

   hnRNPAB X2-RBD
   

   Tags

   6xHis tags-MBP

   Expression

   Bacterial

   Mutation

   deleted amino acids 2-64 and 226-304

   Available Since

   Dec. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJL CusS NarX Chimera

   Plasmid

   #225652

   Purpose

   Expresses a NarX-based chimera capable of sensing copper (II). For use with NarL-YdfI and reporter plasmids from this study.

   Depositor

   Neha Kamat

   Article

   Peruzzi et al Proc Natl Acad Sci U S A. 2023 May 9;120

   Insert

   CusS-NarX
   

   Use

   Cell free expression

   Tags

   Myc

   Expression

   Bacterial

   Mutation

   Transmembrane and sensing domain replaced to dete…

   Promoter

   T7

   Available Since

   Dec. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJL NrsS NarX Chimera

   Plasmid

   #225653

   Purpose

   Expresses a NarX-based chimera capable of sensing nickel (II). For use with NarL-YdfI and reporter plasmids from this study.

   Depositor

   Neha Kamat

   Article

   Peruzzi et al Proc Natl Acad Sci U S A. 2023 May 9;120

   Insert

   NrsS-NarX
   

   Use

   Cell free expression

   Tags

   Myc

   Expression

   Bacterial

   Mutation

   Transmembrane and sensing domain replaced to dete…

   Promoter

   T7

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJL RssA NarX Chimera

   Plasmid

   #225654

   Purpose

   Expresses a NarX-based chimera capable of sensing iron (III). For use with NarL-YdfI and reporter plasmids from this study.

   Depositor

   Neha Kamat

   Article

   Peruzzi et al Proc Natl Acad Sci U S A. 2023 May 9;120

   Insert

   RssA-NarX
   

   Use

   Cell free expression

   Tags

   Myc

   Expression

   Bacterial

   Mutation

   Transmembrane and sensing domain replaced to dete…

   Promoter

   T7

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJL VanS NarX Chimera

    Plasmid

    #225655

    Purpose

    Expresses a NarX-based chimera capable of sensing vancomycin. For use with NarL-YdfI and reporter plasmids from this study.

    Depositor

    Neha Kamat

    Article

    Peruzzi et al Proc Natl Acad Sci U S A. 2023 May 9;120

    Insert

    VanS-NarX
    

    Use

    Cell free expression

    Tags

    Myc

    Expression

    Bacterial

    Mutation

    Transmembrane and sensing domain replaced to dete…

    Promoter

    T7

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSMART-HCKan-yibDp-roGFP2

    Plasmid

    #202462

    Purpose

    Expresses redox sensitive GFP (roGFP) after phosphate depletion

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    reduction-oxidation sensitive green fluorescent protein
    

    Expression

    Bacterial

    Promoter

    yibDp

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. iNicSnFR12

    Plasmid

    #227142

    Purpose

    Genetically encoded fluorescent biosensor for nicotine detection

    Depositor

    Henry Lester

    Article

    Haloi et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

    Insert

    iNicSnFR12
    

    Tags

    6xHis-HA and Myc

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRSF_ApyA

    Plasmid

    #226249

    Purpose

    Production of unmodified ApyA peptide.

    Depositor

    Wilfred van der Donk

    Article

    Nguyen et al ACS Cent Sci. 2024 Apr 11;10(5):1022-103

    Insert

    His6_ApyA
    

    Tags

    Hexa-Histidine

    Expression

    Bacterial

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJB65

    Plasmid

    #226319

    Purpose

    E. coli protein expression for protein purification

    Depositor

    David Savage

    Article

    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

    Insert

    6xHis- (VTG to AAA MR1-6)-strep
    

    Tags

    6xHis and Strep

    Expression

    Bacterial

    Mutation

    N-terminal domain deleted, C-terminal domain dele…

    Promoter

    T7

    Available Since

    Oct. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAMC042z21

    Plasmid

    #209747

    Purpose

    RepA(N99D) SC101 T0-BS7 proD SD8 sfGFP( V93AGGA Y151UAGA) ProK 5 tRNA operon tetA-orfL bla

    Depositor

    Ahmed Badran

    Article

    Costello et al Nat Biotechnol. 2024 Sep 11. doi: 10.103

    Insert

    sfGFP - tRNA operon
    

    Expression

    Bacterial

    Mutation

    sfGFP - Valine 93 AGGA quadruplet codon, Tyrosine…

    Available Since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTE5423_p3WJdB-glcO60

    Plasmid

    #221194

    Purpose

    DNA template of GlcR sensor with glcO60 operator: T7 promoter-glcO60-3WJdB reporter-T7 terminator linear DNA template (PCR amplified with oSB0021+22) is used in the IVT sensor system

    Depositor

    Tobias Erb

    Article

    Barthel et al Synth Biol (Oxf). 2024 Sep 20;9(1):ysae0

    Insert

    T7 promoter-glcO60-3WJdB
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    T7 promoter with downstream glcO60 operator

    Available Since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX-PML RING

    Plasmid

    #185631

    Purpose

    expression of GST-tagged PML RING domain in bacterial cells

    Depositor

    Irina Bezsonova

    Article

    Bregnard et al Structure. 2023 Sep 7;31(9):1086-1099.e6

    Insert

    promyelocytic leukemia protein (PML Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    none

    Available Since

    Sept. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHR-CLIP-PD-L1

    Plasmid

    #223571

    Purpose

    Lentiviral expression of human PD-L1 with CLIP tag in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Cell Rep. 2018 Jul 10;24(2):379-390.e6.

    Insert

    PD-L1 (CD274 Human)
    

    Use

    Lentiviral; mammalian expression

    Tags

    CLIP

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRSET-Cm-CspA1

    Plasmid

    #215398

    Purpose

    Cm NCPPB382 cspA1 codon-optimized for E. coli recombinant protein expression (6xHis-CspA1)

    Depositor

    Gitta Coaker

    Article

    Stevens et al Proc Natl Acad Sci U S A. 2024 Jun 4;121

    Insert

    cspA1
    

    Tags

    6xHis Tag

    Expression

    Bacterial

    Mutation

    Codon-optimized for E. coli BL21(DE3)

    Promoter

    T7 promoter

    Available Since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. T7-LacO-Halo-PILATeS-RGD (200nM)

    Plasmid

    #216716

    Purpose

    Expresses HaloTag-PILATeS-RGD with 200 nM BiT tag in bacteria

    Depositor

    Alex Dunn

    Article

    Zhong et al ACS Sens. 2024 Jul 26;9(7):3489-3495. do

    Insert

    HaloTag-PILATeS-RGD
    

    Tags

    6xHis and HaloTag

    Expression

    Bacterial

    Available Since

    Aug. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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