We narrowed to 7,620 results for: Trac
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Plasmid#233648PurposeExpression vector of AcGFP1 under control of tetracycline responsive element (TRE). Also express Tet-on advenced transactivator (rtTA) under control of minimal CMV promoter.DepositorInserttarget sequence of guide RNA #tyr (3253-3275) 5'-GGCTCCATGTCTTCCGTCCAAGG-3'
UseAmphibian expression, tet-onAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 V5 His-TOPO-GluCl optbeta-m-YFP Y182F RSR_AAA (Opt β-Gluclv2.0)
Plasmid#47542Purposeengineered receptor improves heteromeric receptor expression and sensitivity to IVM.DepositorInsertGlutamate-gated chloride channels
ExpressionMammalianMutationretention motif in ER (starting at R340) changed …PromoterCMVAvailable SinceAug. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAH-CTX1-rhadCas9-native
Plasmid#129392PurposeDerived from pAH-CTX1-rha. The native Streptococcus pyogenes dCas9 gene cloned downstream of PrhaBAD. Non-codon-optimized dCas9 version of pAH-CTX1-rhadCas9.DepositorInsertS. pyogenes dCas9
UseCRISPRExpressionBacterialMutationAspartate 10 to Alanine, Histidine 840 to AlaninePromoterPrhaBADAvailable SinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTC-ApoE-Tet-YAP
Plasmid#85580PurposeSleeping beauty transposon for hydrodynamic tail vein injection for CreER expression and tetracyclin-dependent YAP expression (ApoE.HCR.hAAT promoter) in mouse liverDepositorInsertsCreER
YAP
UseCre/LoxTagsFlag (2x)ExpressionMammalianPromoterApoE.HCR.hAAT and TREAvailable SinceJan. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-pre-pro-alphaf-E2-Crimson
Plasmid#117658PurposeTest intracellular localisation of E2-Crimson and study the secretion efficiencyDepositorInsertpre-pro-alphaf-E2-Crimson
ExpressionYeastMutationThis plasmid encodes the fluorescent protein E2-C…PromoterpAOX1Available SinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
nLuc-ΔECD-PTHR1
Plasmid#190011PurposeExpresses the Nanoluciferase fused to the human parathyroid hormone receptor type 1 that is missing the extracellular domainDepositorInsertnLuc-ΔECD-PTHR1 (PTH1R Human)
ExpressionMammalianAvailable SinceNov. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-hS + eGFP
Plasmid#74945PurposeDosage control and tracing of reprogramming episomesDepositorInsertSox2 (SOX2 Human)
ExpressionMammalianAvailable SinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMflET-o4
Plasmid#101314PurposeContains: complete oriC region of M. florum L1 (oriC4 fragment), colE1 rep origin, recoded tetM and ereB resistance genes, origin of transfer of RP4 plasmid.DepositorInsertsoriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
ereB resistance gene
UseSynthetic BiologyExpressionBacterialAvailable SinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-CD4-TLR4 cyto
Plasmid#133064PurposeRetroviral expression of mouse CD4 extracellular domain fused with the cytosolic tail of TLR4DepositorAvailable SinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 RL3Sc
Plasmid#109365PurposeHuman rod opsin chimera with intracellular loop 3 from Scallop opsin with 1D4 tagDepositorInsertTags1D4ExpressionMammalianPromoterCMVAvailable SinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTripz Casp1-IRES-mCherry
Plasmid#187294PurposeThis is Caspase1 from mouse (NM_009807.2) with its CARD domain replaced by the FKBP-V domain (dimerising domain).DepositorAvailable SinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
cfmTurquoise-N
Plasmid#139284PurposeCys-free mTurquoise (improved ECFP) for extracellular FRET analysis. This variant contains the same set of mutations (C48S and C70M ) as cfSGFP2. Molecular brightness is similar to mTurquoise.DepositorInsertcfmTurquoise
TagsMulti-cloning sitesExpressionMammalianMutationCys48Ser and Cys70/MetAvailable SinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDule-IBBN (G2)
Plasmid#85500PurposePlasmid for incorporating the non-canonical amino acid IBBN with the Mj IBBN (G2) synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsertIBBN (G2) synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
ExpressionBacterialMutationY32G L65E F108W Q109M D158S R162KPromoterlpp (constitutive)Available SinceMarch 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
psgRNA (GB0645)
Plasmid#68210PurposeProvides a Short guide RNA which is the combination of the bacterial crRNA and tracrRNA into a single guide transcript; it does not contain any target siteDepositorInsertSgRNA
UseCRISPR and Synthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceOct. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKS18
Plasmid#24629DepositorAvailable SinceAug. 11, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCMV iGluf
Plasmid#105833PurposeFast indicator for extracellular glutamate imagingDepositorInsertiGluSnFR E25D variant
TagsMyc and PDGFRExpressionMammalianMutationChanged Glu 25 to AspPromoterCMVAvailable SinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
LRP6 dC-pCS2
Plasmid#27258DepositorInsertlow density lipoprotein receptor-related protein 6 (LRP6 Human)
UseXenopus injectionExpressionMammalianMutationcarboxyl intracellular region deleted Residue 14…Available SinceFeb. 10, 2011AvailabilityAcademic Institutions and Nonprofits only -
SEP-Nanoluc-cyOFP1
Plasmid#141082Purposeprototype of the bioluminescent reporter pHLuc, for detecting extracellular pH changes in tumor acidosisDepositorInsertSEP-Nanoluc-cyOFP1
UseLentiviralMutationDeletion of amino acids DISGG from positions 673 …PromoterEF1αAvailable SinceJune 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
CRY2-mCherry-iTrkA
Plasmid#106168Purposemammalian expression of CRY2PHR (aa 1-498) fused to mCherry and intracellular domain of TrkA (aa 450-799)DepositorAvailable SinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a(DAP10)
Plasmid#177904PurposeBacterial expression of his10-tagged human DAP10 Intracellular DomainDepositorInsertDAP10 (aa 70-92 only) (HCST Human)
Tags10xHis-Thrombin cut site-T7 tagExpressionBacterialPromoterT7Available SinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only