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PLX306 iCre tetO tomato rtTA

Plasmid

#158050

Purpose

lenti-viral construct with Cre recombinase and doxycycline inducible expression of tdTomato

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Insert

tdTomato

Tags

none

Expression

Mammalian

Promoter

tetO promoter

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pRRLSIN.cPPT.PGK-GFP-PTS1.WPRE

Plasmid

#133282

Purpose

Lentiviral expression of a peroxisomal eGFP

Depositor

Etienne Sokal

Article

Demaret et al Histochem Cell Biol. 2020 Mar 2. pii: 10

Insert

Peroxisome targeting signal 1

Use

Lentiviral

Tags

Peroxisome targeting signal 1

Expression

Mammalian

Promoter

None

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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aL-EGFP

Plasmid

#103879

Purpose

full-length human aL with EGFP at betapropeller

Depositor

Timothy Springer

Article

Nordenfelt et al Nat Commun. 2017 Dec 11;8(1):2047. doi:

Insert

full-length human aL with EGFP in betapropeller (ITGAL Human)

Tags

tension sensor module

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pUDE731

Plasmid

#103008

Purpose

S. cerevisiae Episomal plasmid harboring Fncpf1 under the control of TDH3p

Depositor

Jean-Marc Daran

Article

Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

Insert

Fncpf1

Tags

3HA and NLS

Expression

Yeast

Mutation

human codon optimized

Promoter

TEF1

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pPB-PGK-hKLF4

Plasmid

#60435

Purpose

Over-expression of human KLF4

Depositor

Austin Smith

Article

Takashima et al Cell. 2014 Sep 11;158(6):1254-69. doi: 1

Insert

KLF4 (KLF4 Human)

Expression

Mammalian

Available Since

May 16, 2016

Availability

Academic Institutions and Nonprofits only

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pSIREN-RetroQ-shN

Plasmid

#73665

Purpose

pSIREN-RetroQ vector containing control sequence N

Depositor

Joe Landry

Article

Mayes et al Oncotarget. 2017 May 12;8(38):64344-6435

Insert

shRNA Neg Control

Use

Retroviral

Available Since

March 22, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pLH-stsgRNA1.1

Plasmid

#64116

Purpose

Vector for expression of St1 sgRNA1.1 in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

St1 sgRNA1.1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR MCS L3-L2

Plasmid

#62088

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a multiple cloning site. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

Gateway Cloning

Expression

Mammalian

Available Since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only

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pDC32

Plasmid

#171204

Purpose

bacterial expression for HIS purification of aa1-698 of MEG-3

Depositor

Geraldine Seydoux

Article

Schmidt et al bioRxiv 2020.10.15.340570

Insert

meg-3 (meg-3 Nematode)

Tags

6xHIS

Expression

Bacterial

Mutation

aa1-698

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pAAP20

Plasmid

#171206

Purpose

bacterial expression for HIS purification of the MEG-3 C-terminus

Depositor

Geraldine Seydoux

Article

Schmidt et al bioRxiv 2020.10.15.340570

Insert

meg-3 (meg-3 Nematode)

Tags

8xHIS

Expression

Bacterial

Mutation

aa545-862

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pDC25

Plasmid

#171203

Purpose

bacterial experssion of a GST-tagged MEG-3 Cterminus

Depositor

Geraldine Seydoux

Article

Schmidt et al bioRxiv 2020.10.15.340570

Insert

meg-3 (meg-3 Nematode)

Tags

GST

Expression

Bacterial

Mutation

aa545-862

Available Since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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Zeta-globin

Plasmid

#19262

Depositor

Philip Leder

Article

Leder et al Nature. . 293(5829):196-200.

Insert

zeta globin (Hba-x Mouse)

Expression

Bacterial

Available Since

Sept. 22, 2008

Availability

Academic Institutions and Nonprofits only
pHW530 (Prab-3::cGAL-N::let-858 3'UTR)

Plasmid

#107131

Purpose

cGAL-N driver under the control of C. elegans rab-3 promoter

Depositor

Paul Sternberg

Article

Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

Insert

NLS::cGAL(DBD)::gp41-1-N-intein

Expression

Worm

Promoter

C. elegans rab-3 promoter

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pBPhsFlp2::PEST

Plasmid

#63171

Purpose

Drosophila codon-optimized Flp2::mODC-PEST fusion gene driven from Drosophila Hsp70Bb Heat Shock Promoter

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

Insert

hsFlp2::PEST

Expression

Insect

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-BRX-eGFP-NLS

Plasmid

#217534

Purpose

AAV vector to drive the expression of eGFP under the control of the 4xBRE regulatory element of SMAD1 and miniXon splicing casette transcription factor in vivo

Depositor

Peter Scheiffele

Article

Okur et al bioRxiv 2023

Insert

BMP reporter element and miniXon casette (Smad1 Mouse)

Use

AAV

Expression

Mammalian

Promoter

BMP Reporter Element

Available Since

April 11, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsRoquin1_AE

Plasmid

#148611

Purpose

Mammalian Expression of HsRoquin1

Depositor

Elisa Izaurralde

Article

Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

Insert

HsRoquin1 (RC3H1 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-EF1a-MegaGate-DD-Blast

Plasmid

#175271

Purpose

MegaGate destination with C-terminal DD tag and blasticidin resistance

Depositor

George Church

Article

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Tags

Destabilization Domain

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pPAP004

Plasmid

#160911

Purpose

E.coli/ P.pastoris shuttle plasmid for episomal expression in P.pastoris

Depositor

Sylvestre Marillonnet

Article

Pullmann et al ACS Synth Biol. 2021 Jun 2. doi: 10.1021

Insert

lacZ selection cassette

Expression

Yeast

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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Myo 19-GFP

Plasmid

#134988

Purpose

Expression of human Myo 19 in mammalian cells.

Depositor

Martin Bähler

Article

Oeding et al J Cell Sci. 2018 Sep 10;131(17). pii: jc

Insert

Myo 19 (MYO19 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV IE

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW ytdTomato

Plasmid

#118456

Purpose

Overexpression of yeast codon-optimized tdTomato

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ytdTomato

Expression

Yeast

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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