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We narrowed to 9,944 results for: Gnas

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Showing: 561 - 580 of 9944 results

  3. pcDNA5/FRT/TO-GAlpha11-RLuc8

    Plasmid
    #140983
    Purpose
    Encodes a G alpha subunit (GNA11) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlpha11-RLuc8 (GNA11 Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140983-plasmid-map-sequence-id-300767

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  4. pcDNA5/FRT/TO-GAlphasL-RLuc8

    Plasmid
    #140981
    Purpose
    Encodes a G alpha subunit (GNAS1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlphasL-RLuc8 (GNAS Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140981-plasmid-map-sequence-id-300681

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  5. pcDNA5/FRT/TO-GAlphaoA-RLuc8

    Plasmid
    #140976
    Purpose
    Encodes a G alpha subunit (GNAO1 Isoform Alpha 1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlphaoA-RLuc8 (GNAO1 Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140976-plasmid-map-sequence-id-300659

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  6. pcDNA5/FRT/TO-GAlphai2-RLuc8

    Plasmid
    #140974
    Purpose
    Encodes a G alpha subunit (GNAI2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlphai2-Rluc8 (GNAI2 Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140974-plasmid-map-sequence-id-300657

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  7. pcDNA5/FRT/TO-GAlphaoB-RLuc8

    Plasmid
    #140977
    Purpose
    Encodes a G alpha subunit (GNAO1 Isoform Alpha 2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlphaoB-Rluc8 (GNAO1 Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140977-plasmid-map-sequence-id-300660

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  9. pAAV hSyn INTRON mEmerald-ER WPRE

    Plasmid
    #236230
    Purpose
    AAV expression of a fluorescent marker, mEmerald fused to Prolactin signal peptide and KDEL sequence; for expression and retention in the lumen of the endoplasmic reticulum
    Depositor
    Jennifer Lippincott-Schwartz
    Article
    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do
    Insert
    mEmerald-PRL signal peptide-KDEL sequence (PRL Bovine)
    Use
    AAV
    Tags
    mEmerald
    Promoter
    human Synapsin 1
    Available Since
    May 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    236230-plasmid-map-sequence-id-471247

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  10. pIL6-PMEL-hbb

    Plasmid
    #239868
    Purpose
    Template for in vitro transcription of PMEL (gp100), with signal peptide replaced by the IL6 signal peptide, flanked by the human haemoglobin beta 5' and 3' UTRs.
    Depositor
    Catherine Jopling
    Article
    Smart et al Mol Ther Nucleic Acids. 2025 Jun 24;36(3
    Insert
    Full premelanosome coding sequence with IL6 signal peptide, flanked by human haemoglobin beta 5' and 3' UTRs (PMEL Human)
    Expression
    Mammalian
    Mutation
    Valine to Glycine change is at amino acid 26 of n…
    Promoter
    T7 (included as part of insert)
    Available Since
    July 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    239868-plasmid-map-sequence-id-477826

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  11. G12-CASE

    Plasmid
    #190714
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein G12. Composed of the subunits G alpha 12 (GNA12) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).
    Depositor
    Gunnar Schulte
    Article
    Schihada et al J Biol Chem. 2022 Aug 3:102328. doi: 10.
    Inserts
    GNB3 (GNB3 Human)
    GNGT9
    GNA12 (GNA12 Human)
    Use
    Luciferase
    Tags
    Nluc is inserted at A133/F134 within GNA12 and cp…
    Expression
    Mammalian
    Available Since
    Dec. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    190714-plasmid-map-sequence-id-379753

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  13. pMG051 MBP-β-catenin-His, GST-CK1α

    Plasmid
    #154072
    Purpose
    Co-expresses MBP-β-catenin-His (human β-catenin as a fusion protein with MBP and His- tags) and GST_CK1α in E.coli to produce primed MBP-β-catenin-His
    Depositor
    Jesse Zalatan
    Article
    Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.
    Inserts
    CTNNB1 (CTNNB1 Human)
    CSNK1A1 (CSNK1A1 )
    Tags
    GST, His6, and MBP
    Expression
    Bacterial
    Promoter
    Tac
    Available Since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    154072-plasmid-map-sequence-id-308806

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  15. TRUPATH Triple Gai2

    Plasmid
    #196049
    Purpose
    Encodes a G alpha subunit (GNAl2) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphai2-RLuc8 (GNAI2 Human)
    GGamma8-GFP2 (GNG8 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196049-plasmid-map-sequence-id-385863

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  16. TRUPATH Triple Ga11

    Plasmid
    #196059
    Purpose
    Encodes a G alpha subunit (GNA11) with RLuc8, a G gamma subunit (GNG13) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlpha11-RLuc8 (GNA11 Human)
    GGamma13-GFP2 (GNG13 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    Feb. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    196059-plasmid-map-sequence-id-386126

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  17. TRUPATH Triple Ga15

    Plasmid
    #196060
    Purpose
    Encodes a G alpha subunit (GNA15) with RLuc8, a G gamma subunit (GNG13) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlpha15-RLuc8 (GNA15 Human)
    GGamma13-GFP2 (GNG13 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196060-plasmid-map-sequence-id-385870

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  18. SV.control

    Plasmid
    #133932
    Purpose
    Serves as a control in tests involving digital signatures
    Depositor
    Jean Peccoud
    Insert
    lacZ
    Expression
    Bacterial
    Available Since
    Jan. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    133932-plasmid-map-sequence-id-268620

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  19. pMSCV-IRES-Blue FP

    Plasmid
    #52115
    Purpose
    a bicistronic IRES-Blue FP containing retroviral vector
    Depositor
    Dario Vignali
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    IRES-Blue FP
    Expression
    Mammalian
    Available Since
    April 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    52115-plasmid-map-sequence-id-351562

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  20. pICherryNeo

    Plasmid
    #52119
    Purpose
    a bicistronic IRES-mCherry containing vector for mammalian expression (CMV promoter)
    Depositor
    Dario Vignali
    Type
    Empty backbone
    Tags
    IRES-mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    52119-plasmid-map-sequence-id-80505

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Showing: 561 - 580 of 9944 results