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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 28,440 results for: REP

Showing: 8671 - 8700 of 28440 results

  4. pJK2067

    Plasmid
    #171845
    Purpose
    C. elegans mex-5 promoter (germline) driven dual fluorescent reporter for boxB tethering (eGFP) with boxB negative control (mCherry)
    Depositor
    Judith Kimble
    Article
    Aoki et al Nat Commun. 2021 Feb 12;12(1):996. doi:
    Insert
    his-58, eGFP, mCherry
    Use
    Tags
    N-terminal Tag OLLAS, V5 and C-terminal Tag PEST…
    Expression
    Worm
    Mutation
    boxB wild type and hairpin mutant
    Promoter
    mex-5 promoter
    Available since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    171845-plasmid-map-sequence-id-337181

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  7. Antares-SEPLuc

    Plasmid
    #141086
    Purpose
    precursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis
    Depositor
    Jeak Ling Ding
    Article
    Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41
    Insert
    Antares-T2A-puromycin-EMCV IRES-SEPLuc
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Deletion of amino acids 66 to 91 on the Luciferas…
    Promoter
    CMV
    Available since
    June 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    141086-plasmid-map-sequence-id-296862

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  15. pCfB2188-PrARG8-GFP::URA3

    Plasmid
    #216512
    Purpose
    Integrative fluorescent reporter for amino acid biosynthesis sensing pathways, comprising GFP driven by the ARG8 promoter within the Easy clone vector, requires NotI digestion for integration in yeast
    Depositor
    Juha Saarikangas
    Article
    Paukstyte et al Mol Biol Cell. 2025 Apr 2:mbcE24040162.
    Insert
    PrARG8-yeGFP
    Use
    Cre/Lox; Integrative
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ARG8
    Available since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    216512-plasmid-map-sequence-id-429384

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  16. pCfB2197-PrGNP1-mK2-NatMX

    Plasmid
    #216513
    Purpose
    Integrative fluorescent reporter for amino acid uptake sensing pathway, comprising mKate2 driven by the GNP1 promoter within the Easy clone vector, requires NotI digestion for integration in yeast
    Depositor
    Juha Saarikangas
    Article
    Paukstyte et al Mol Biol Cell. 2025 Apr 2:mbcE24040162.
    Insert
    PrGnp1-mKate2
    Use
    Cre/Lox; Integrative
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GNP1
    Available since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    216513-plasmid-map-sequence-id-429383

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  17. pEW107

    Plasmid
    #232796
    Purpose
    COMPASS Fragment 2 (His-FLAG-ySet1, Cps30, Twinstrep-Cps40) in pBIG2ab
    Depositor
    Cynthia Wolberger
    Article
    Worden et al Elife. 2020 Jan 10;9. pii: 53199. doi: 1
    Insert
    COMPASS Fragment 2 (His-FLAG-ySet1, Cps30, Twinstrep-Cps40) (SET1, SPP1, SWD3 Budding Yeast)
    Use
    Tags
    His6-3xFLAG, Twinstrep
    Expression
    Insect
    Mutation
    ySet1(762-1080)
    Promoter
    polyhedrin promoter
    Available since
    March 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    232796-plasmid-map-sequence-id-465733

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  22. pHPHS926

    Plasmid
    #228237
    Purpose
    Bxb1 attB_GA mCherry-T2A-puro, Dox-inducible β-globin NMD reporter with premature termination codon for integration into Bxb1 attP_GA landing pad
    Depositor
    Arvind Rasi Subramaniam
    Article
    Nugent et al bioRxiv [Preprint]. 2024 Jul 26:2024.07.
    Inserts
    mCherry
    Beta globin
    Use
    Tags
    3xFLAG, HA, and PuroR
    Expression
    Mammalian
    Mutation
    PTC39
    Promoter
    TRE3GV and none
    Available since
    Jan. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    228237-plasmid-map-sequence-id-455212

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  23. pWR58

    Plasmid
    #202795
    Purpose
    integrating bioluminescene reporter for Cas9 activity in S. stipitis
    Depositor
    J. Brian Robertson
    Article
    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:
    Inserts
    P-TDH3/coCBG(first portion)-YUM1
    P-TEF1/ShBle/T-ACT1
    YUM1-coCBG(last portion)/T-ADH2
    URA3 and flanking sequence
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Dec. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    202795-plasmid-map-sequence-id-455380

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  30. pro::EX1-2(intΔNACs):GFP

    Plasmid
    #218570
    Purpose
    Transcriptional reporter for ARF7 promoter
    Depositor
    Anthony Bishopp
    Insert
    AT5G20730 (NPH4 Mustard Weed)
    Use
    Tags
    Expression
    Plant
    Mutation
    Mutation genomic sequence 85nt, 86nt from TA to C…
    Promoter
    Available since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    218570-plasmid-map-sequence-id-438438

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Showing: 8671 - 8700 of 28440 results