We narrowed to 30,619 results for: ica

1. MTK234_032

   Plasmid

   #123995

   Purpose

   Encodes the spCas9 sgRNA1 hAAVS1 (site 1) gRNA expression cassette as a type 234 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   sgRNA1 -hAAVS1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pTriEx4-H6-FGB

   Plasmid

   #49203

   Purpose

   Fluorescent reporter for cGMP

   Depositor

   John Garthwaite

   Article

   Bhargava et al Front Mol Neurosci. 2013 Sep 24;6:26. do

   Insert

   H6-FGB
   

   Use

   Tags

   His tag + Other tags

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   None

   Promoter

   CMV

   Available since

   Dec. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGBW-m4046806

   Plasmid

   #145746

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 M (membrane)

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;6xHIS

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   April 28, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pJH4531

   Plasmid

   #132366

   Purpose

   Prgef-1 DAF-2 unc-54 3' UTR C.elegans pan-neural expression of DAF-2. This plasmid has replaced plasmid pJH664 from the Zhen Lab.

   Depositor

   Mei Zhen

   Article

   Hung et al Development. 2014 Apr;141(8):1767-79. do

   Insert

   DAF-2 (daf-2 Nematode)
   

   Use

   Tags

   none

   Expression

   Worm

   Mutation

   Promoter

   Prgef-1

   Available since

   Sept. 22, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pRG/III-hs-MBP-T43NTR-TrxA-H10

   Plasmid

   #121677

   Purpose

   Bacterial expression of neurotensin receptor fusion protein

   Depositor

   Richard Henderson, Reinhard Grisshammer

   Article

   Grisshammer et al Protein Expr Purif. 1997 Oct;11(1):53-60

   Insert

   neurotensin receptor (Ntsr1 Rat)
   

   Use

   Tags

   TrxA-H10

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-42

   Promoter

   lac

   Available since

   March 5, 2019

   Availability

   Academic Institutions and Nonprofits only

6. pCR8-Magneto2.0

   Plasmid

   #74333

   Purpose

   For gateway cloning Magneto2.0 into destination vector

   Depositor

   Ali Guler

   Article

   Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/n

   Insert

   Magneto2.0
   

   Use

   Gateway vector

   Tags

   Flag

   Expression

   Mutation

   TRPV4: delta 760-871

   Promoter

   none

   Available since

   July 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. L40C-CRISPR.EFS.dTomato

   Plasmid

   #89392

   Purpose

   Lentiviral CRISPR-Cas9 delivery for sgRNA (hU6), dTomato coexpression, EFS Promoter driven

   Depositor

   Dirk Heckl

   Article

   Reimer et al Haematologica. 2017 Jun 1. pii: haematol

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV.hSynap.iGABASnFR.F102Y.Y137L

   Plasmid

   #112161

   Purpose

   GABA Sensor

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

   Insert

   iGABASnFR.F102Y.Y137L
   

   Use

   AAV

   Tags

   Expression

   Mutation

   F102Y.Y137L

   Promoter

   hSynapsin

   Available since

   July 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMH4

   Plasmid

   #52529

   Purpose

   serves as PCR-template to generate HR donors for C-terminal 2xFLAG-tag

   Depositor

   Klaus Foerstemann

   Article

   Bottcher et al Nucleic Acids Res. 2014 Apr 19.

   Insert

   2x FLAG-tag
   

   Use

   Blunt cloning kit

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJW4.16

    Plasmid

    #62871

    Purpose

    Bacterial expression of MBP-MBK-2

    Depositor

    Geraldine Seydoux

    Article

    Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

    Insert

    MBP-MBK-2
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1-GFP-MTn-KDEL

    Plasmid

    #154348

    Purpose

    Mammalian expression of MTn

    Depositor

    Wanzhong He

    Article

    Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

    Insert

    MTn
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV.GFAP.iGABASnFR.F102Y.Y137L

    Plasmid

    #112174

    Purpose

    GABA Sensor

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

    Insert

    iGABASnFR.F102Y.Y137L
    

    Use

    AAV

    Tags

    Expression

    Mutation

    F102Y.Y137L

    Promoter

    GFAP

    Available since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCytERM-LSSmGFP

    Plasmid

    #186540

    Purpose

    Visualization of the ER in cells using LSSmGFP (ex/em = 400/510 nm); compatible with OSER assay.

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    LSSmGFP
    

    Use

    Tags

    Cytochrome p450 first 29 animo acids (Oryctolagus…

    Expression

    Mammalian

    Mutation

    hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K

    Promoter

    CMV

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. mEmerald-MCM3-N-22

    Plasmid

    #54166

    Purpose

    Localization: Nucleus, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Article

    Kuipers et al J Cell Biol. 2011 Jan 10;192(1):29-41. d

    Insert

    MCM3
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

15. GFP-NPM NESD

    Plasmid

    #13283

    Depositor

    Xin Wang

    Article

    Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

    Insert

    NPM (NPM1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Deletion of aa94-aa102

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

16. pHiUGE V5-epitope donor ORF0

    Plasmid

    #200377

    Purpose

    Tagging endogenously expressed proteins with V5 epitope, C-terminal

    Depositor

    Scott Soderling

    Article

    Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

    Insert

    V5-epitope
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pKMV-VioC

    Plasmid

    #65349

    Purpose

    systhesis of vioC gene in the violacein pathway

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    vioC
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pER-eZinCh-2

    Plasmid

    #70009

    Purpose

    expresses ER-eZinCh-2 Zn2+ FRET sensor in the ER of mammalian cells

    Depositor

    Maarten Merkx

    Article

    Hessels et al ACS Chem Biol. 2015 Sep 18;10(9):2126-34

    Insert

    ER-eZinCh-2
    

    Use

    Tags

    KDEL sequence and PPI signal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MO70 Orai1 N223A

    Plasmid

    #21663

    Depositor

    Anjana Rao

    Article

    Gwack et al J Biol Chem. 2007 Jun 1. 282(22):16232-4

    Insert

    Orai1 (ORAI1 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    N223A

    Promoter

    Available since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

20. Danio rerio cryaba

    Plasmid

    #47620

    Purpose

    Recombinant protein production

    Depositor

    Mason Posner

    Article

    Posner et al PLoS One. 2012;7(3):e34438. doi: 10.1371

    Insert

    Alpha Ba-crystallin (cryaba Zebrafish)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. EGFP-Rab8b in pCSDEST2

    Plasmid

    #140881

    Purpose

    Expression of zebrafish GFP-tagged Rab8b in mammalian cells.

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    Rab8b (rab8b Zebrafish)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. FRB-polySH33-EGFP

    Plasmid

    #175792

    Purpose

    Bacterial expression and purification, fluorescently tagged scaffold for droplet visualization and volume quantification

    Depositor

    Michael Rosen

    Article

    Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

    Insert

    NCK1 (NCK1 Human)
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. MAC_C_EPHB6

    Plasmid

    #187763

    Purpose

    MAC-tagged gene expression of human EPHB6

    Depositor

    Markku Varjosalo

    Article

    Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

    Insert

    EPHB6 (EPHB6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. CROP_C9_Puro_FLT3

    Plasmid

    #183293

    Purpose

    All-in-One CRISPRko system with a guide RNA that targets FLT3 gene

    Depositor

    Prashant Mali

    Article

    Hu et al Biomaterials. 2022 Jan;280:121276. doi:

    Insert

    FLT3
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. 3xFRB-myc

    Plasmid

    #20226

    Depositor

    Carolyn Bertozzi

    Article

    Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

    Insert

    3xFRB (MTOR Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    1 FRB copy has a T2098L point mutation

    Promoter

    Available since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

26. MTK234_033

    Plasmid

    #123996

    Purpose

    Encodes the spCas9 sgRNA1 hAAVS1 (site 2) gRNA expression cassette as a type 234 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    sgRNA2 -hAAVS1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. MTK234_034

    Plasmid

    #123997

    Purpose

    Encodes the spCas9 sgRNA1 hAAVS1 (site 3) gRNA expression cassette as a type 234 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    sgRNA3 -hAAVS1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAG306-pTet07-synYFP[TTG/AGA]-nLuc

    Plasmid

    #199756

    Purpose

    Elongation reporter construct to quantify elongation of synYFP[TTG]; Control construct of elongation reporter to quantify elongation duration of n_CGA stalling

    Depositor

    Brian Zid

    Article

    Hou et al bioRxiv. 2023 Mar 20:2023.03.19.533377.

    Insert

    synYFP[TTG/AGA]-nLuc
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TetO7

    Available since

    May 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. MTK0_031

    Plasmid

    #123970

    Purpose

    Encodes the BxBI-hygromycin-mRuby2PEST landing pad as a type 0 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    BxBI-hygro-mRuby2PEST landing
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pXL001

    Plasmid

    #26122

    Depositor

    Sean Palecek

    Article

    Lian et al Proc Natl Acad Sci U S A. 2012 May 29.

    Insert

    tTR-KRAB
    

    Use

    Lentiviral

    Tags

    IRES-Puro

    Expression

    Mutation

    Promoter

    Available since

    June 7, 2012

    Availability

    Academic Institutions and Nonprofits only

31. GFP/WT SP M85

    Plasmid

    #89317

    Purpose

    Expression of mouse GFP/WT spastin M85 in mammalian cells

    Depositor

    Peter Baas

    Article

    Solowska et al J Neurosci. 2008 Feb 27;28(9):2147-57. d

    Insert

    SPAST M85 (Spast Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. CROP_C9_Puro_CSF1R

    Plasmid

    #183277

    Purpose

    All-in-One CRISPRko system with a guide RNA that targets CSF1R gene

    Depositor

    Prashant Mali

    Article

    Hu et al Biomaterials. 2022 Jan;280:121276. doi:

    Insert

    CSF1R
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pJH3185

    Plasmid

    #132367

    Purpose

    Prgef-1 GFP::DAF-16a unc-54 3' UTR C.elegans pan-neural expression of isoform a of DAF-16 fused to GFP

    Depositor

    Mei Zhen

    Article

    Hung et al Development. 2014 Apr;141(8):1767-79. do

    Insert

    DAF-16a (daf-16 Nematode)
    

    Use

    Tags

    GFP

    Expression

    Worm

    Mutation

    Promoter

    Prgef-1

    Available since

    Oct. 28, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

34. attB-GFP

    Plasmid

    #65521

    Purpose

    Plasmid for the generation of GFP-knockin fusions via Bxb1-mediated recombination into MIN-tagged cell lines

    Depositor

    Sebastian Bultmann

    Article

    Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

    Insert

    attB-GFP
    

    Use

    Mouse Targeting; Bxb1

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pET21c-IF3mt

    Plasmid

    #31078

    Depositor

    Linda Spremulli

    Article

    Koc et al J Biol Chem. 2002 Sep 20. 277(38):35541-

    Insert

    Mature form human mitochondrial translational initiation factor 3 (MTIF3 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Includes coding sequence for the mature form. Ami…

    Promoter

    Available since

    Aug. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

36. c-myc promoter (4x TBE2 - wt)

    Plasmid

    #16599

    Depositor

    Bert Vogelstein

    Article

    He et al Science. 1998 Sep 4. 281(5382):1509-12.

    Insert

    c-myc promoter (4xTBE2 wt) (MYC Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2008

    Availability

    Academic Institutions and Nonprofits only

37. pT7-agrBD-I

    Plasmid

    #53439

    Purpose

    T7-dependent expression of the type-I agrB and agrD genes of S. aureus RN4220

    Depositor

    Cynthia Collins

    Article

    Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

    Insert

    agrBD locus
    

    Use

    Synthetic Biology

    Tags

    V5 epitope, 6x HIS (not expressed on agrB or agrD…

    Expression

    Bacterial

    Mutation

    none

    Promoter

    T7-lac

    Available since

    June 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. p6609 MSCV-IP N-HAonly PTENP1

    Plasmid

    #35008

    Depositor

    Peter Howley

    Article

    White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

    Insert

    PTENP1 (PTENP1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV LTR

    Available since

    Feb. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

39. nGR2175-gs10-NLS-VPR

    Plasmid

    #188264

    Purpose

    Plasmid ensures constitutive expression of the N-terminal fragment of split GR2 (aa 1-175), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminus

    Depositor

    Roman Jerala

    Article

    Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

    Insert

    nGR2175
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pDOC-K-glmS-mPlum

    Plasmid

    #158062

    Purpose

    Donor plasmid for insertion of mPlum and the constitutive acpP promoter into the S. Typhimurium chromosome. mPlum is in the forward orientation relative to the kanamycin resistance selectable marker

    Depositor

    Mark Webber

    Article

    Holden et al Microbiology (Reading). 2020 Nov 23. doi

    Insert

    monomeric plum fluoresecent protein
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    acpP

    Available since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge