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3341 pcDNA3 Bcl-2 T69A S70A S87A

Plasmid

#13340

Depositor

Stanley Korsmeyer

Publication

Yamamoto et al Mol Cell Biol 1999 Dec;19(12):8469-78.

Insert

Bcl-2 (BCL2 Human)

Expression

Mammalian

Mutation

changed Threonine 69 to Alanine; changed Serine 7…

Available since

Nov. 9, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pQE60-Exp5

Plasmid

#12553

Depositor

Ian Macara

Publication

Brownawell et al J Cell Biol. 2002 Jan 7. 156(1):53-64.

Insert

EXPORTIN-5 (XPO5 Human)

Tags

HIS

Expression

Bacterial

Mutation

NONE

Available since

Oct. 2, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
pCS

Plasmid

#12158

Depositor

Inder Verma

Publication

Miyoshi et al J Virol. 1998 Oct . 72(10):8150-7.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLVPRT-tTR-KRAB

Plasmid

#11648

Purpose

Tet-regulated (Tet-on) lentiviral vector for transgene (hPrion promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

Depositor

Patrick Aebischer, Didier Trono

Publication

Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

Insert

hPrion, GFP, tTR-KRAB, Tet-on

Use

Lentiviral

Expression

Mammalian

Available since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pACT2.2gtwy

Plasmid

#11346

Depositor

Guy Caldwell

Insert

gateway cassette

Tags

Gal4 Activation Domain

Expression

Yeast

Available since

July 19, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles
FLAG.PKCepsilon.K/W

Plasmid

#10796

Depositor

Alex Toker

Publication

Cenni et al Biochem J. 2002 May 1. 363(Pt 3):537-45.

Insert

PKC epsilon (PRKCE Human)

Tags

FLAG

Expression

Mammalian

Mutation

Kinase dead mutant: K437W (Lys 437 to Trp)

Available since

July 18, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
Stat4 pRc/CMV

Plasmid

#8711

Depositor

Jim Darnell

Publication

Zhong et al Proc Natl Acad Sci U S A 1994 May 24;91(

Insert

Stat4 (Stat4 Mouse)

Expression

Mammalian

Available since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

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pFastBac1 Flag BAF155 b

Plasmid

#1954

Depositor

Robert Kingston

Publication

Phelan et al Mol Cell 1999 Feb;3(2):247-53.

Insert

BAF155 (SMARCC1 Human)

Tags

flag

Expression

Insect

Available since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
1247 pCMVb p300 HA delta33

Plasmid

#10719

Depositor

William Sellers

Insert

p300 delta33 (EP300 Human)

Tags

HA

Expression

Mammalian

Mutation

Mutant does not bind to E1A. Amino acids 1737 to…

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pRS420

Plasmid

#11259

Depositor

Fred Cross

Type

Empty backbone

Expression

Yeast

Available since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pCMV_ENVLPE+

Plasmid

#232427

Purpose

Expression plasmid to produce ENVLPE+ particles

Depositor

Gil Westmeyer

Publication

Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

Insert

ENVLPE+

Expression

Mammalian

Promoter

2xCMV

Available since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-hSyn-GRAB-rDA3m

Plasmid

#208702

Purpose

Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3m in neurons

Depositor

Yulong Li

Publication

Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

GPCR activation based dopamine (DA) sensor GRAB_rDA3m

Use

AAV

Promoter

hSyn

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pS-sg:GFP

Plasmid

#196296

Purpose

35S promoter-driven expression of the positive-sense TSWV S RNA segment encoding sgRNA:GFP fusion in place of viral NSs

Depositor

Zhenghe Li

Publication

Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

Insert

Full length TSWV S antigenome encoding sgRNA:GFP fusion in place of viral NSs

Use

CRISPR

Expression

Plant

Promoter

duplicated cauliflower mosaic virus 35S promoter

Available since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-Ad5RGDPK7

Plasmid

#198291

Purpose

Produces PVCs retargeted with expanded tropism hAd5 knob (RGD and PK7 insertions)

Depositor

Feng Zhang

Publication

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-Ad5RGDPK7

Expression

Bacterial

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pmScarlet3-Giantin_C1

Plasmid

#189773

Purpose

Construct for labelling Golgi apparatus in mammalian cells using mScarlet3-Giantin fusion

Depositor

Dorus Gadella

Publication

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mScarlet3-Giantin

Expression

Mammalian

Promoter

CMV

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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AAVS1-Bxb1-LP-v2-TC

Plasmid

#194326

Purpose

AAVS1 targeting construct with the Bxb1 landing pad version 2 cassette.

Depositor

Richard Davis

Publication

Blanch-Asensio et al Stem Cell Res. 2023 Feb;66:102991. doi:

Insert

loxP-EBFP2-attP-BleoR-lox251

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pCW empty vector

Plasmid

#184708

Purpose

Lentiviral empty vector for doxycycline-inducible expression

Depositor

Alessia Ciarrocchi, Gloria Manzotti

Publication

Gugnoni et al J Exp Clin Cancer Res. 2022 Mar 25;41(1)

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pCMV GFP1-9

Plasmid

#182244

Purpose

Tripartite split-GFP mammalian. Express GFP1-9 detector fragment

Depositor

Stéphanie Cabantous

Publication

Cabantous et al Sci Rep. 2013 Oct 4;3:2854. doi: 10.1038

Insert

GFP 1-9 OPT

Expression

Mammalian

Promoter

CMV

Available since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHAGE-mKeima-LGALS3

Plasmid

#175780

Purpose

Lentiviral vector expressing N-terminally tagged mKeima-LGALS3 (Lyso-Keima), for quantitative assessment of lysophagic flux

Depositor

Wade Harper

Publication

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

LGALS3 (LGALS3 Human)

Use

Lentiviral

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMOS014: Grx1-roGFP2 glutathione redox sensor (cytosolic)

Plasmid

#163055

Purpose

Grx1-roGFP2 glutathione redox sensor (cytosolic)

Depositor

Adam Cohen

Publication

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

Grx1-roGFP2

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST-eGFP-C1-VPS35

Plasmid

#163622

Purpose

human VPS35 cDNA C-terminally tagged with eGFP

Depositor

Josef Kittler

Publication

Halff et al Cell Rep. 2019 Nov 26;29(9):2599-2607.e6

Insert

human VPS35 (VPS35 Human)

Tags

GFP

Expression

Mammalian

Available since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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Green Pegassos

Plasmid

#163114

Purpose

Expresses biosensors for pyruvate in mammalian cells

Depositor

Takashi Tsuboi

Publication

Harada et al Sci Rep. 2020 Nov 11;10(1):19562. doi: 1

Insert

Green Pegassos

Expression

Mammalian

Promoter

CMV

Available since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pcDNA3 FlipGFP(Mpro) T2A mCherry

Plasmid

#163078

Purpose

Expresses FlipGFP-based activity reporter of SARS-CoV-2 main protease Mpro and also mCherry

Depositor

Xiaokun Shu

Publication

Li et al PLoS Pathog. 2021 Sep 3;17(9):e1009898.

Insert

FlipGFP-based Mpro activity reporter and mCherry

Expression

Mammalian

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMK265 (RAD21-mAC Hygro)

Plasmid

#140539

Purpose

RAD21 tagging with mAID-Clover

Depositor

Masato Kanemaki

Publication

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

RAD21 (RAD21 Human)

Use

CRISPR

Expression

Mammalian

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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m6A-Tracer-NES

Plasmid

#159607

Purpose

Constitutive transient mammalian expression of m6A-Tracer protein (Kind et al. 2013) with a C-terminal HIV-1 Rev Nuclear Export Signal to reduce background due to nonspecific DNA interactions.

Depositor

Aaron Streets

Publication

Altemose et al Cell Syst. 2020 Oct 21;11(4):354-366.e9.

Insert

m6A-Tracer-NES

Expression

Mammalian

Promoter

CMV

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-S5E2-GFP-fGFP

Plasmid

#135631

Purpose

AAV vector to drive the expression of fGFP in PV cortical interneuronsunder the control of the E2 regulatory element

Depositor

Jordane Dimidschstein

Publication

Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

Has service

AAV1 and AAV9

Insert

GFP-fGFP

Use

AAV

Mutation

None

Available since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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CARPID dCasRx-BASU

Plasmid

#153303

Purpose

express CARPID dCasRx-BASU fusion protein in mammalian cells

Depositor

Jian Yan, Liang Zhang

Publication

Yi et al Nat Methods. 2020 Jun 22. pii: 10.1038/s

Insert

dCasRx-BASU

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

R239A/H244A/R858A/H863A in RfxCas13d

Promoter

EF-1a

Available since

June 29, 2020

Availability

Academic Institutions and Nonprofits only

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CMV-mito-Citron1

Plasmid

#134305

Purpose

mitochondria-targeted direct-response citrate sensor

Depositor

Robert Campbell

Publication

Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

Insert

Citron1

Expression

Mammalian

Promoter

CMV

Available since

April 13, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pEF-BFP

Plasmid

#138272

Purpose

Expression of engineered BFP variant that undergoes conversion to GFP after targeted modification with a cytidine deaminase-based DNA base editor

Depositor

Xiao Wang

Publication

Standage-Beier et al Nucleic Acids Res. 2019 Aug 20. pii: 555

Insert

BFP H66 PAM

Use

CRISPR

Expression

Mammalian

Mutation

H66, PAM motif

Promoter

Ef1a

Available since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pQE-60NA-msGFP2

Plasmid

#135301

Purpose

Encodes a photostable monomeric superfolder GFP

Depositor

Benjamin Glick

Publication

Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

Insert

msGFP2

Expression

Bacterial

Promoter

T5

Available since

Jan. 15, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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