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p300dAIL KAT LIC 1M

Plasmid

#233588

Purpose

Bacterial expression of p300 KAT enzyme with truncated autoinhibitory loop

Depositor

Scott Showalter

Article

Dewing et al Proteins. 2025 Jun 1. doi: 10.1002/prot.

Insert

p300dAIL KAT (EP300 Human)

Tags

6xHis MBP

Expression

Bacterial

Mutation

deleted amino acids 1523-1554

Promoter

T7

Available Since

June 2, 2025

Availability

Academic Institutions and Nonprofits only

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pET21a-LysRS-His

Plasmid

#124114

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Lysine-tRNA ligase (lysU Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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p53-GFP

Plasmid

#11770

Depositor

Geoff Wahl

Insert

p53 (TP53 Human)

Tags

GFP

Expression

Mammalian

Mutation

72R variant of p53

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only

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pC015 - dLwCas13a-NF

Plasmid

#91905

Purpose

Expresses negative-feedback dLwCas13a for mammalian RNA targeting/imaging

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

dLwCas13a

Use

CRISPR

Tags

NLS and eGFP, ZF CCR5TC, KRAB-A, NLS

Expression

Mammalian

Mutation

R474A and R1046A

Promoter

CAG

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pPSU250

Plasmid

#173837

Purpose

Expresses 250 kD Penn State ladder protein

Depositor

Song Tan

Article

Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

Insert

STRHSTPABPACIL1bQRSSTRHSTPABMBPpepN

Expression

Bacterial

Promoter

T7

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV3flag8SHARPIN

Plasmid

#50014

Purpose

Mammalian expression of human SHARPIN with a c-terminal 3xFLAG tag, and hygromycin selection.

Depositor

Martin Dorf

Article

Fu et al Cell Res. 2013 Sep 24. doi: 10.1038/cr.2

Insert

SHANK-associated RH domain interactor (SHARPIN Human)

Tags

3 x FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 31, 2013

Availability

Academic Institutions and Nonprofits only

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pBS-EF1afull-H2B-mCherry-P2A-Fluc

Plasmid

#207253

Purpose

Lentiviral transfer genome encoding H2B-mCherry-P2A-Fluc driven by a full-length EF1a promoter

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

H2B-mCherry-P2A-Fluc

Use

Lentiviral

Expression

Mammalian

Promoter

EF1alpha_full

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pFLAG-HHARI

Plasmid

#17450

Depositor

Dong-Er Zhang

Article

Okumura et al Genes Dev. 2007 Feb 1. 21(3):255-60.

Insert

HHARI (ARIH1 Human)

Tags

Flag

Expression

Mammalian

Available Since

March 5, 2008

Availability

Academic Institutions and Nonprofits only

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pUCKanR-Mu-BsaI

Plasmid

#79769

Purpose

Transposon-propagation plasmid for Mu-BsaI modified transposon

Depositor

David Savage

Article

Nadler et al Nat Commun. 2016 Jul 29;7:12266. doi: 10

Insert

Mu-BsaI

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pMito-miRFP703

Plasmid

#80000

Purpose

Mitochondria labeling with near-infrared fluorescent protein miRFP703

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

cytochrome C oxidase subunit VIII (COX8A Human)

Tags

miRFP703

Expression

Mammalian

Promoter

CMV

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-EGFP-NLS-FLAG-cGAS

Plasmid

#127659

Purpose

Over-express EGFP-NLS-FLAG-cGAS

Depositor

Nicolas Manel

Article

Gentili et al Cell Rep. 2019 Feb 26;26(9):2377-2393.e1

Insert

cGAS (CGAS Human)

Use

Lentiviral

Tags

EGFP-NLS-Flag

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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Pet28a_Human_H4

Plasmid

#42633

Depositor

Joe Landry

Insert

Human H4 (H4C1 Human)

Tags

Untagged

Expression

Bacterial

Promoter

T7

Available Since

Feb. 25, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pAV5B+longBRD4

Plasmid

#157792

Purpose

Expression of long isoform of BRD4

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

long BRD4 (BRD4 Human)

Tags

His6

Expression

Insect

Promoter

polyhedron

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Zeo-moxGFP

Plasmid

#207699

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-moxGFP cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pTRE-BI-hGRAD

Plasmid

#207837

Purpose

Inducible hGRAD Plasmids for rapid depletion of GFP-tagged proteins in mammalian cells

Depositor

Michaela Mueller-McNicoll

Article

Arnold et al J Cell Biol. 2024 Feb 5;223(2):e20230403

Inserts

mCherry
hFBXW11 (FBXW11 Human)
VHH-GFP4

Expression

Mammalian

Mutation

215 amino acids of the full length protein

Promoter

TRE3G BI promoter

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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AICSDP-15:ACTB-mEGFP

Plasmid

#87425

Purpose

Homology arms and linker-mEGFP sequence for N-terminus tagging of human ACTB

Depositor

Allen Institute for Cell Science

Article

Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

Insert

ACTB Homology Arms with mEGFP-linker (ACTB Human)

Use

CRISPR; Donor template

Tags

mEGFP-linker

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Available Since

March 21, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3-AKAR4-Kras

Plasmid

#61621

Purpose

Non-raft/plasma membrane-targeted FRET biosensor for monitoring PKA activity

Depositor

Jin Zhang

Article

Depry et al Mol Biosyst. 2011 Jan;7(1):52-8. doi: 10

Insert

AKAR4-Kras

Tags

C-terminal targeting sequence from Kras, Cerulean…

Expression

Mammalian

Promoter

CMV

Available Since

March 13, 2015

Availability

Academic Institutions and Nonprofits only

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mEGFP-RhoA-C1

Plasmid

#29674

Depositor

Ryohei Yasuda

Article

Murakoshi et al Nature. 2011 Apr 7;472(7341):100-4. doi:

Insert

RhoA (RHOA Human)

Tags

mEGFP

Expression

Mammalian

Available Since

June 6, 2011

Availability

Academic Institutions and Nonprofits only

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pDisplay-GRAB-rDA3h-IRES-EGFP-CAAX

Plasmid

#208697

Purpose

Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3h and a membrane-localized EGFP in mammalian cells

Depositor

Yulong Li

Article

Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

GPCR activation based dopamine (DA) sensor GRAB_rDA3h

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pBTK1126

Plasmid

#236187

Purpose

BsaI compatible gentamicin resistance cassette

Depositor

Jeffrey Barrick

Article

VanDieren et al MicroPubl Biol. 2025 Aug 2;2025:10.17912

Insert

gentamicin resistance cassette

Expression

Bacterial

Promoter

CP25

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pEGFP-MRLC T18A,S19A

Plasmid

#35681

Depositor

Tom Egelhoff

Article

Beach et al BMC Cell Biol. 2011 Dec 2;12:52.

Insert

MRLC1 (MYL9 Human)

Tags

EGFP

Expression

Mammalian

Mutation

T18A and S19A

Promoter

CMV

Available Since

April 24, 2012

Availability

Academic Institutions and Nonprofits only
pAAV-CAG-NIR-GECO2

Plasmid

#159603

Purpose

AAV production plasmid encoding for near-infrared calcium indicator NIR-GECO2

Depositor

Robert Campbell

Article

Qian et al PLoS Biol. 2020 Nov 24;18(11):e3000965.

Insert

NIR-GECO2

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-Drosha

Plasmid

#62520

Purpose

wild-type human Drosha cDNA is subcloned into pEGFP-C1 vector between HindIII and KpnI sites

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha full length (DROSHA Human)

Tags

GFP

Expression

Mammalian

Available Since

March 20, 2015

Availability

Academic Institutions and Nonprofits only

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PGK EGFP

Plasmid

#216110

Purpose

PGK EGFP

Depositor

Erika Holzbaur

Article

Dou et al J Cell Biol. 2024 Jun 3;223(6):e20230709

Insert

PGK promoter

Tags

EGFP

Expression

Mammalian

Promoter

PGK

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn1-GCaMP6f-P2A-nls-dTomato

Plasmid

#51085

Purpose

Neuronal expression of GCaMP6f Ca sensor and physically separate nuclear localized dTomato fluorophore

Depositor

Jonathan Ting

Has Service

AAV Retrograde and AAV1

Insert

GCaMP6f

Use

AAV

Tags

P2A-nls-dTomato

Expression

Mammalian

Promoter

human Synapsin1

Available Since

Feb. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pCS2+mNeonGreen-C Cloning Vector

Plasmid

#128144

Purpose

To express a protein of interest fused to the C-terminus of mNeonGreen

Depositor

Ken-Ichi Takemaru

Type

Empty backbone

Tags

mNeonGreen fusion

Expression

Mammalian

Available Since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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pCF806-shRen.713

Plasmid

#186712

Purpose

Expresses dox-controlled miR-E shRNAs (UT4GEPIR). Non-targeting control shRNA (shRen.713).

Depositor

Christof Fellmann

Article

Amen et al Cell Rep Methods. 2022 Jun 21;2(7):10023

Insert

shRen.713

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-gfap(Intron1/5'/Exon1-zebrafish)

Plasmid

#39761

Depositor

Pamela Raymond

Article

Bernardos et al Gene Expr Patterns. 2006 Oct;6(8):1007-1

Insert

zebrafish gfap promoter- EGFP (gfap Zebrafish)

Use

Zebrafish expression

Promoter

gfap

Available Since

Aug. 21, 2012

Availability

Academic Institutions and Nonprofits only

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PLJR965

Plasmid

#115163

Purpose

Sth1 dCas9 TetR and KanR L5 Int attP for M. tuberculosis

Depositor

Sarah Fortune

Article

Rock et al Nat Microbiol. 2017 Feb 6;2:16274. doi:

Inserts

Sth1 sgRNA scaffold
Sth1 dCas9
TetR (deleted TetON) MtbCO
L5 attP
L5 Int
KanR

Use

Unspecified

Available Since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA4TO-24xGCN4_v4-kif18b-24xPP7

Plasmid

#74928

Purpose

Translation reporter

Depositor

Marvin Tanenbaum

Article

Yan et al Cell. 2016 May 5;165(4):976-89. doi: 10.

Insert

24xGCN4_v4-kif18b-24xPP7

Expression

Mammalian

Available Since

May 10, 2016

Availability

Academic Institutions and Nonprofits only

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