159,530 results

1. GFP-BRD3

   Plasmid

   #65377

   Purpose

   GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

   Depositor

   Kyle Miller

   Article

   Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

   Insert

   BRD3 (BRD3 Human)
   

   Use

   Tags

   EmGFP and V5

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   May 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAVS1-pur-CAG-mCherry

   Plasmid

   #159458

   Purpose

   AAVS1 targeting donor plasmid with mCherry expression cassette and puromycin selection gene

   Depositor

   Yuejun Chen

   Article

   Xiong et al Cell Stem Cell. 2020 Sep 16. pii: S1934-

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Dec. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. 6His-MBP-TEV-huLbCpf1

   Plasmid

   #90096

   Purpose

   Bacterial expression plasmid for protein purification

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

   Insert

   huLbCpf1
   

   Use

   Tags

   3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. CAG-ADAR2-sesRNA-GFP-W3SL

   Plasmid

   #192069

   Purpose

   Expression of ADAR2, sesRNA in mammalian cells, with tTA2 as efRNA and ADAR2 overexpression.

   Depositor

   Josh Huang

   Article

   Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

   Insert

   sesRNA for mouse Ctip2
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. GST-RBD

   Plasmid

   #15247

   Depositor

   Martin Schwartz

   Article

   Ren et al EMBO J. 1999 Feb 1. 18(3):578-85.

   Insert

   Rhotekin (Rtkn Mouse)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   amino acids 7-89 (Rho binding domain)

   Promoter

   Available since

   Sept. 13, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLKO-puro-tetON-shRFP

   Plasmid

   #110940

   Purpose

   Tet-inducible shRNA targeting RFP

   Depositor

   William Hahn

   Article

   Ilic et al Proc Natl Acad Sci U S A. 2017 Apr 25;11

   Insert

   shRFP
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mutation

   Promoter

   H1/TO

   Available since

   Nov. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNIR-FbGFP

   Plasmid

   #184675

   Purpose

   NIR-Fb to GFP

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

   Insert

   NIR-FbGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCFD4-U6:1_U6:3tandemgRNAs

   Plasmid

   #49411

   Purpose

   expression of two gRNAs from Drosophila U6:1 and U6:3

   Depositor

   Simon Bullock

   Article

   Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

   Inserts

   dU6-1:gRNA
   dU6-3:gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   dU6-1 and dU6-3

   Available since

   Jan. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGreen2x35SLUC

   Plasmid

   #108228

   Purpose

   Plant expression as translational fusions to FLUC with RLUC normalization

   Depositor

   Alejandro Ferrando

   Article

   Solé-Gil et al Methods Mol Biol. 2024;2722:79-87. doi:

   Type

   Empty backbone

   Use

   Luciferase

   Tags

   Firefly Luciferase

   Expression

   Plant

   Mutation

   Promoter

   2x35S

   Available since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pExpreS2-2

    Plasmid

    #175362

    Purpose

    G418R backbone for recombinant protein overexpression with Drosophila ExpreS2 Platform

    Depositor

    ExpreS2ion Biotechnologies

    Article

    Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Fused Actin-HSP70

    Available since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX601-AAV-CMV-NLS-ISAam1-NLS-3xHA-bGHpA;U6-BbsI-reRNA

    Plasmid

    #205316

    Purpose

    All-in-one AAV vector expressing ISAam1 TnpB and its reRNA

    Depositor

    Haoyi Wang

    Article

    Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

    Insert

    humanized ISAam1 TnpB
    

    Use

    AAV

    Tags

    3 x HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3-HA-CUL1 K720A

    Plasmid

    #19939

    Depositor

    Yue Xiong

    Article

    Furukawa et al Mol Cell Biol. 2000 Nov . 20(21):8185-97

    Insert

    cullin 1 K720A (CUL1 Human)
    

    Use

    Tags

    HA2

    Expression

    Mammalian

    Mutation

    CUL1 with a NEDD8 modification mutant

    Promoter

    Available since

    April 17, 2009

    Availability

    Academic Institutions and Nonprofits only

13. FLAG-HA-pcDNA3.1-

    Plasmid

    #52535

    Purpose

    enables eukaryotic expression of N-terminally FLAG-HA-tagged fusion proteins

    Depositor

    Adam Antebi

    Article

    Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMSP3545

    Plasmid

    #46888

    Purpose

    Gram-Positive Bacterial Shuttle Vector for Nisin-Controlled Inducible Expression

    Depositor

    Gary Dunny

    Article

    Bryan et al Plasmid. 2000 Sep;44(2):183-90.

    Type

    Empty backbone

    Use

    Nisin-inducible expression; gram-positive bacteri…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PnisA

    Available since

    Sept. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pQC membrane TdTomato IX

    Plasmid

    #37351

    Depositor

    Connie Cepko

    Article

    Beier et al Dev Biol. 2011 May 15;353(2):309-20. Epu

    Insert

    membrane TdTomato
    

    Use

    Tags

    Palmitoylation signal*

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGFP-ninein C-ter

    Plasmid

    #73523

    Purpose

    Encodes the C-terminus of mouse ninein (residues 1874-2113) with an EGFP tag at its N-terminus

    Depositor

    Michel Bornens

    Article

    Delgehyr et al J Cell Sci. 2005 Apr 15;118(Pt 8):1565-7

    Insert

    ninein C-ter (residues 1874-2113) (Nin Mouse)
    

    Use

    Tags

    EGFP tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 29, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pActP-GCaMP6m

    Plasmid

    #64714

    Purpose

    visualizing calcium in cultured Drosophila S2 cells

    Depositor

    Liqun Luo

    Article

    Gao et al Nat Neurosci. 2015 May 11. doi: 10.1038/

    Insert

    GCaMP6m
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEGFP-hCB2R

    Plasmid

    #179322

    Purpose

    Express human cannabinoid 2 receptor (GFP tagged) in mammalian cells

    Depositor

    Christian Gruber

    Article

    Tomašević et al J Biol Chem. 2024 Jun;300(6):107330. doi

    Insert

    human cannabinoid 2 receptor (CNR2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Lenti-FAB(ER-mito)

    Plasmid

    #214423

    Purpose

    Detection of ER-mito contact site

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    RspAN-FRB-V5-Sec61_IRES_CFAST10-20nm-Halo-mito
    

    Use

    Lentiviral

    Tags

    CFAST10-Halo and RspAN-FRB-V5

    Expression

    Mutation

    Promoter

    CMV

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. Flag-Axin1

    Plasmid

    #109370

    Purpose

    Transient over-expression of human Axin1

    Depositor

    Mariann Bienz

    Article

    Fiedler et al Proc Natl Acad Sci U S A. 2011 Feb 1;108

    Insert

    Axin1 (AXIN1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSL-MS2-6X

    Plasmid

    #27118

    Depositor

    Robert Singer

    Article

    Bertrand et al Mol Cell. 1998 Oct . 2(4):437-45.

    Insert

    MS2 binding sites (6)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pX601-GFP

    Plasmid

    #84040

    Purpose

    Staphylococcus aureus (SaCas9) conjugated with GFP

    Depositor

    Yuet Wai Kan

    Article

    Ye et al Proc Natl Acad Sci U S A. 2016 Sep 20;11

    Insert

    SaCas9
    

    Use

    AAV and CRISPR

    Tags

    NLS and T2A-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3β-FLAG-CBP-HA

    Plasmid

    #32908

    Depositor

    Tso-Pang Yao

    Article

    Zhao et al Mol Cell Biol. 2005 Oct;25(19):8456-64.

    Insert

    CBP (Crebbp Mouse)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA4-FLAG-Jhdm2a-H1122A

    Plasmid

    #38140

    Depositor

    Toshinobu Nakamura, Toru Nakano

    Article

    Nakamura et al Nature. 2012 Jun 3;486(7403):415-9. doi:

    Insert

    Jhdm2a-H1122A (Kdm3a Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    protein starts at aa115; H1122A

    Promoter

    CMV

    Available since

    Sept. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pSpCas9-2A-Puro-RAB7A-gRNA1 (PX459)

    Plasmid

    #221551

    Purpose

    Expresses Cas9 and gRNA for disruption of Rab7A gene in human cells

    Depositor

    Shawn Ferguson

    Article

    Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

    Insert

    gRNA targeting human Rab7A (RAB7A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBABE BRG1

    Plasmid

    #1959

    Depositor

    Robert Kingston

    Article

    Sif et al Genes Dev 2001 Mar 1;15(5):603-18.

    Insert

    BRG1 (SMARCA4 Human)
    

    Use

    Retroviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    E1262K R1575Q

    Promoter

    Available since

    March 23, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLVX-EF1a-GFP-LaminA-IRES-Hygromycin

    Plasmid

    #134867

    Purpose

    Expresses EGFP-tagged nuclear envelope marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    Lamin A (LMNA Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pME-Kaede

    Plasmid

    #132972

    Purpose

    full-length of photoconvertible kaede with stop codon in pmE Gateway vector

    Depositor

    Nathan Lawson

    Article

    Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

    Insert

    Kaede
    

    Use

    Gateway vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCMV-Neo-Bam APC 1-2644

    Plasmid

    #16511

    Depositor

    Bert Vogelstein

    Article

    Morin et al Science. 1997 Mar 21. 275(5307):1787-90.

    Insert

    APC (APC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Codon 2644 truncation

    Promoter

    Available since

    April 30, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET11phiC31poly(A)

    Plasmid

    #18942

    Depositor

    Michele Calos

    Article

    Groth et al Genetics. 2004 Apr . 166(4):1775-82.

    Insert

    phiC31 integrase (int Streptomyces phage phiC31)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 13, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge