We narrowed to 10,611 results for: t7

1. pcDNA3 HA-SUMO2-Q90P

   Plasmid

   #36448

   Purpose

   Expresses HA-Sumo2Q90P mutant in mammalian cells

   Depositor

   Guy Salvesen

   Article

   Bekes et al J Biol Chem. 2011 Mar 25;286(12):10238-4

   Insert

   SUMO2 (SUMO2 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Mutated Q90P

   Promoter

   T7

   Available Since

   Oct. 23, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pET-6His-MBP-MpAgo

   Plasmid

   #79249

   Purpose

   This plasmid encodes the Argonaute protein from Marinitoga Piezophila and is codon optimized for E. coli expression

   Depositor

   Jennifer Doudna

   Article

   Kaya et al Proc Natl Acad Sci U S A. 2016 Mar 30. p

   Insert

   Marinitoga piezophila Argonaute
   

   Use

   CRISPR

   Tags

   6XHis-tag + MBP + PreScission Cleavage Site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET22b_PstS_3

   Plasmid

   #78200

   Purpose

   Expression of double-cysteine variant of mature phosphate binding protein, PstS, which also has a mutation to weaken phosphate binding, as scaffold for phosphate biosensor

   Depositor

   Martin Webb

   Article

   Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62

   Insert

   pstS (A17C,I76G,A197C)
   

   Expression

   Bacterial

   Mutation

   A17C,I76G,A197C (please see depositor comment bel…

   Promoter

   T7

   Available Since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPagP

   Plasmid

   #112125

   Purpose

   Bacterial expression of outer membrane protein PagP, wild type

   Depositor

   Karen Fleming

   Article

   Burgess et al J Biol Chem. 2008 Sep 26;283(39):26748-5

   Insert

   PagP (pagP E. coli)
   

   Expression

   Bacterial

   Mutation

   N-terminal signal sequence not included and repla…

   Promoter

   T7

   Available Since

   Nov. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET22b-PelB-6H-Ag43-160N-sfGFP

   Plasmid

   #172886

   Purpose

   Display sfGFP protein on E. coli cell surface

   Depositor

   Urartu Seker

   Article

   Ahan et al ACS Synth Biol. 2019 Apr 19;8(4):686-696

   Inserts

   SfGFP
   Ag43, partial (flu )
   

   Tags

   HHHHHH and TEV recognition site (ENLYFQG)

   Expression

   Bacterial

   Mutation

   Deleted 1-160 (N terminal)

   Promoter

   T7 Promoter

   Available Since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. PVS10

   Plasmid

   #104398

   Purpose

   polycistronic vector for expression of E. coli core RNAP

   Depositor

   Irina Artsimovitch

   Article

   Svetlov et al Methods Mol Biol. 2015;1276:13-29. doi:

   Insert

   rpoA-rpoB-rpoC-6xHis and rpoZ (rpoA, rpoB, rpoC, rpoZ E. coli)
   

   Tags

   His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNCS mRFP

   Plasmid

   #91765

   Purpose

   Bacterial expression of a fluorescent protein, monomeric Red Fluorescent Protein1 (mRFP1)

   Depositor

   Erik Rodriguez, Roger Tsien

   Article

   Campbell et al Proc Natl Acad Sci U S A. 2002 Jun 11;99

   Insert

   monomeric Red Fluorescent Protein1
   

   Tags

   Hexa-Histidine tag, Xpress epitope for detection …

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a-TS2126 RnlA-Strep

   Plasmid

   #159350

   Purpose

   Express TS2126 RNA ligase with His6 tag and Strep tag at N and C terminal, respectively

   Depositor

   Takashi Ito

   Article

   Hisano et al BMC Biol. 2021 Oct 14;19(1):225. doi: 10

   Insert

   TS2126 RNA ligase
   

   Tags

   His6 and Strep

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-28a(+)::NL

   Plasmid

   #141289

   Purpose

   NLUC in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)

   Depositor

   Andrew Millar

   Article

   Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

   Insert

   NLUC
   

   Use

   Luciferase

   Expression

   Bacterial

   Mutation

   Engineered for high stability (t1/2 = 11.5 days a…

   Promoter

   promoter for bacteriophage T7 RNA polymerase

   Available Since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCellFree_G03 CYP2D6

    Plasmid

    #67090

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    CYP2D6 (CYP2D6 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMSP1E2D1

    Plasmid

    #161606

    Purpose

    Bacterial expression of "extended" membrane scaffold protein MSP1E2D1 for Nanodisc formation

    Depositor

    Stephen Sligar

    Article

    Ritchie et al Methods Enzymol. 2009;464:211-31. doi: 1

    Insert

    MSP1E2D1
    

    Tags

    7-His

    Expression

    Bacterial

    Mutation

    "extended" MSP1D1; contains repeat of h…

    Promoter

    T7

    Available Since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3 MYC-Sumo3Q89P

    Plasmid

    #48963

    Purpose

    Expresses MYC-Sumo3Q90P mutant in mammalian cells

    Depositor

    Guy Salvesen

    Article

    Bekes et al J Biol Chem. 2011 Mar 25;286(12):10238-4

    Insert

    SUMO3 (SUMO3 Human)
    

    Tags

    MYC (EQKLISEED)

    Expression

    Mammalian

    Mutation

    Mutated Q89P

    Promoter

    T7

    Available Since

    Nov. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

13. Pediculus humanus corporis PINK1 (115 - 575)

    Plasmid

    #110750

    Purpose

    Bacterial expression of PhPINK1 (biochemistry)

    Depositor

    David Komander

    Article

    Schubert et al Nature. 2017 Dec 7;552(7683):51-56. doi:

    Insert

    Phum_PHUM577390 (Phum_PHUM577390 Pediculus humanus corporis)
    

    Tags

    N-His6-GST-3C

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pT22-GluCNZ-6His

    Plasmid

    #213504

    Purpose

    Designed for cytoplasmic E.coli overexpression of glucanase (β-1,3-glycosidase) for gentle yeast spheroblasting that unlike Zymolyase prevents proteolysis of intracellular components.

    Depositor

    John Denu

    Article

    Kuznetsov et al J Biol Chem. 2018 Aug 3;293(31):12271-12

    Insert

    Glucanase (glucan endo-1,3-beta-glucosidase)
    

    Tags

    6His tag

    Expression

    Bacterial

    Mutation

    1-36 Signal Sequence Deletion

    Promoter

    T7

    Available Since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRSETb-pcDronpa2

    Plasmid

    #78184

    Purpose

    Expresses pcDronpa2 in E. coli strains

    Depositor

    Peter Dedecker

    Article

    Moeyaert et al ACS Nano. 2014 Feb 25;8(2):1664-73. doi:

    Insert

    pcDronpa2
    

    Tags

    6xHis-tag; Xpress tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. His6_MBP_FMRP_P451S

    Plasmid

    #198693

    Purpose

    Expression of human FMRP protein in E. coli

    Depositor

    Simpson Joseph

    Article

    Edwards et al Sci Rep. 2020 Sep 28;10(1):15858. doi: 1

    Insert

    FMR1 (FMR1 Human)
    

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    changed proline 451 to serine

    Promoter

    T7

    Available Since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFS_0453_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)_T7term_ARRAY2_FaqI

    Plasmid

    #117006

    Purpose

    Expresses E. coli codon-optimized FsRT-Cas1-Cas2 under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout

    Depositor

    Randall Platt

    Article

    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

    Insert

    Fusicatenibacter saccharivorans RT-Cas1-Cas2
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET21d-myc-BirA

    Plasmid

    #109424

    Purpose

    Bacterial expression of biotin ligase (BirA) with an N-terminal myc epitope

    Depositor

    Martin Spiess

    Article

    Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

    Insert

    BirA (birA )
    

    Tags

    myc

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET22b - PA

    Plasmid

    #84863

    Purpose

    bacterial expression of Bacillus anthracis protective antigen

    Depositor

    Russell Vance

    Article

    Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

    Insert

    PA
    

    Tags

    pelB signal sequence

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-Venus-CAAX

    Plasmid

    #87612

    Purpose

    Plasma membrane-targeted Venus (YFP).

    Depositor

    Jin Zhang

    Article

    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

    Insert

    Venus-CAAX
    

    Tags

    6xHis, C-terminal targeting sequence from Kras, T…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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