164,649 results

1. Lenti-sgNT.2#2/Cre

   Plasmid

   #173661

   Purpose

   Expresses a NT.2-targeting gRNA and Cre-recombinase

   Depositor

   Charles Swanton

   Article

   Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

   Insert

   Non-targeting gRNA
   

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRK5-HA-Parkin

   Plasmid

   #17613

   Depositor

   Ted Dawson

   Article

   Zhang et al Proc Natl Acad Sci U S A 2000 Nov 21;97(

   Insert

   Parkin (PRKN Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hsyn-DIO-Rpl22l1-3Flag-2A-eGFP-WPRE

   Plasmid

   #214265

   Purpose

   Expression of a Flag-tagged Rpl22I1 protein in cells expressing Cre recombinase allowing for the harvesting of actively transcribed mRNAs in that cell type.

   Depositor

   D. James Surmeier

   Insert

   Flag-tagged Rpl22I1 protein expressing Cre Recombinase (Rpl22 Mouse)
   

   Use

   AAV

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Synapsin

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRDB_182

   Plasmid

   #216103

   Purpose

   CRISPRi, EF1a-driven dCas9-KRAB (ZIM3) (Cas only)

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Insert

   Cas9 [Sp]
   

   Use

   CRISPR and Lentiviral; Assembled vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOPINVHH_his_flag

   Plasmid

   #210404

   Purpose

   For expression of VHH with C-terminal his and Flag tags

   Depositor

   Ray Owens

   Article

   Eyssen et al Bio Protoc. 2024 Mar 20;14(6):e4962. doi

   Type

   Empty backbone

   Use

   Tags

   his6-flag

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available Since

   March 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYES260-sc.eEF2

   Plasmid

   #85119

   Purpose

   yeast expression of EEF2

   Depositor

   Pal Falnes

   Article

   Davydova et al J Biol Chem. 2014 Oct 31;289(44):30499-5

   Insert

   EFT1/EFT2 (EFT2, EFT1 Budding Yeast)
   

   Use

   Tags

   6xHis

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available Since

   Nov. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. SnailHA_pcDNA3

   Plasmid

   #31697

   Depositor

   Paul Wade

   Article

   Kajita et al Mol Cell Biol. 2004 Sep . 24(17):7559-66

   Insert

   Snail (SNAI1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUPD2 RDF ( GB1498)

   Plasmid

   #160579

   Purpose

   Streptomyces phage PhiC31-encoded recombination directionality factor (RDF) (PhiC31 gp3)

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

   Insert

   RDF
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available Since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pdCas9-DNMT3A-EGFP

   Plasmid

   #71666

   Purpose

   Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-EGFP for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA

   Depositor

   Vlatka Zoldos

   Article

   Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

   Insert

   S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A Human, S. pyogenes, Synthetic)
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, and T2A-EGFP

   Expression

   Mammalian

   Mutation

   D10A and H840A in S.pyogenes Cas9

   Promoter

   CBh

   Available Since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBE48

    Plasmid

    #174656

    Purpose

    CAPTURE method BAC origin of replication receiver plasmid

    Depositor

    Huimin Zhao

    Article

    Enghiad et al Nat Commun. 2021 Feb 19;12(1):1171. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDONR223-DAPK2

    Plasmid

    #23390

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    DAPK2 (DAPK2 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

12. mNG2(1-10)_tdTomato

    Plasmid

    #184169

    Purpose

    Lentiviral vector for expression and selection of the mNG2(1-10) split fluorescent protein.

    Depositor

    Ophir Shalem

    Article

    Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

    Inserts

    mNG2(1-10)
    tdTomato
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEF 3-YFP-2

    Plasmid

    #20106

    Depositor

    Antonio Richart

    Insert

    YFP
    

    Use

    Synthetic Biology; Plant gateway vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 11, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBad-CA TAG126

    Plasmid

    #105836

    Purpose

    Expresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 126 and C-terminal 6x his tag

    Depositor

    Ryan Mehl

    Article

    Mehl et al 2020

    Insert

    human carbonic anhydrase II thermostable variant (CA2 Human)
    

    Use

    Tags

    6x his tag

    Expression

    Bacterial

    Mutation

    TAG stop codon at site 126

    Promoter

    pBad/arabinose

    Available Since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTCF158 Human beta-2 microglobulin

    Plasmid

    #180457

    Purpose

    E. coli expression of class I MHC light chain

    Depositor

    John Altman

    Insert

    Human beta-2 microglobulin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV-CMV-EGFP-Hygro

    Plasmid

    #184593

    Purpose

    EGFP

    Depositor

    Robert Sobol

    Article

    Koczor et al Methods Mol Biol. 2023;2609:43-59. doi:

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. TDP-43_NTD1-80_E17R

    Plasmid

    #104479

    Purpose

    bacterial expression of TDP-43 NTD E17R

    Depositor

    Nicolas Fawzi

    Article

    Wang et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    E17R

    Promoter

    Available Since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX-RCC1-HA

    Plasmid

    #106938

    Purpose

    Transient expression of HA-tagged RCC1 in mammalian cells (CMV promoter)

    Depositor

    Patrizia Lavia

    Article

    DeLuca et al Cancer Res. 2003 Mar 15;63(6):1430-7.

    Insert

    human RCC1-HA (RCC1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available Since

    March 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR223_HAVCR2_WT

    Plasmid

    #82893

    Purpose

    Gateway Donor vector containing HAVCR2, part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, Matthew Meyerson

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    HAVCR2 (HAVCR2 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    R140L

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLVX-EF1a-EGFP-TA Golgin84-IRES-Puromycin

    Plasmid

    #133028

    Purpose

    Expresses EGFP-tagged Golgi apparatus marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    Golgin84 (GOLGA5 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-673

    Promoter

    Human elongation factor 1 alpha

    Available Since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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