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pKLV-EF1a-BFP-W

Plasmid

#208757

Purpose

Lentiviral vector expressing BFP

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Type

Empty backbone

Use

Lentiviral

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pET15b PKA Cat

Plasmid

#14921

Depositor

Susan Taylor

Article

Narayana et al Biochemistry. 1997 Apr 15. 36(15):4438-4

Insert

PKA catalytic subunit alpha (Prkaca Mouse)

Tags

6xHis and Thrombin cleavage site

Expression

Bacterial

Available Since

July 24, 2007

Availability

Academic Institutions and Nonprofits only

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TLR4

Plasmid

#20863

Depositor

Jie-Oh Lee

Article

Park et al Nature. 2009 Apr 30;458(7242):1191-5. do

Insert

Toll-Like Receptor 4 (TLR4 Human)

Expression

Insect

Mutation

Contains ectodomain (amino acids 27–631)

Available Since

May 20, 2009

Availability

Academic Institutions and Nonprofits only

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mCherry-PAK2 binding domain

Plasmid

#83951

Purpose

FLIM acceptor for mEGFP Rac1

Depositor

Ryohei Yasuda

Article

Hedrick et al Nature. 2016 Sep 28;538(7623):104-108. d

Insert

Pak2 (PAK2 Human)

Tags

mCherry

Expression

Mammalian

Mutation

aa60-113 only; S74A F84A

Available Since

Nov. 22, 2016

Availability

Academic Institutions and Nonprofits only

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Lamp1-mScarlet-I

Plasmid

#98827

Purpose

In vivo visualization of lysosomes (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

Lamp1

Tags

mScarlet-I

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 14, 2017

Availability

Academic Institutions and Nonprofits only

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P362-FBP_12

Plasmid

#139703

Purpose

T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pSlH4:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pAtuMas:NhH3H:tAtuMas, p35S:NnHisps:t35S, p35S:NnLuz:AtuOcs

Use

Luciferase

Expression

Plant

Promoter

various

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pUPD2

Plasmid

#68161

Purpose

Universal domesticator plasmid for level 0 parts

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

empty vector

Use

Synthetic Biology; Domestication of dna parts for…

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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CFP_ betaGlobin_24XPP7_24XMS2_Splicing_ reporter

Plasmid

#61762

Purpose

Dual Color betaGlobin reporter to study splicing kinetics in vivo

Depositor

Daniel Larson

Article

Coulon et al Elife. 2014 Oct 1;3. doi: 10.7554/eLife.

Insert

b-Globin

Tags

24 X MS2 hairpin cassette in 3'UTR of b-Glob…

Expression

Mammalian

Promoter

Dox inducible Tet-ON promoter

Available Since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

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pVP074

Plasmid

#222077

Purpose

eforRED visually marked kanamycin-resistant pVS1 plasmid backbone containing the sacB eviction marker and an excisable ccdb/CmR cassette flanked by EcoRV-HindIII restriction sites.

Depositor

Wayne Parrott

Article

Pennetti et al Biodes Res. 2025 Feb 27;7(1):100001. doi

Type

Empty backbone

Use

Chromoprotein-marked pvs1 backbone

Available Since

Jan. 27, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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mScarletI-Rab7

Plasmid

#112955

Purpose

In vivo visualization of Rab7 (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

Rab7

Tags

mScarlet-I

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 26, 2018

Availability

Academic Institutions and Nonprofits only

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ABE8r

Plasmid

#208294

Purpose

Expresses ABE8r in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA8r-SpCas9 D10A

Expression

Mammalian

Mutation

W23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106V, …

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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EB3-mScarlet-I

Plasmid

#98826

Purpose

In vivo visualization of microtuble plus ends (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

EB3

Tags

mScarlet-I

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pLX_311-KRAB-dCas9

Plasmid

#96918

Purpose

for CRISPRi, lentiviral expression of KRAB-dCas9 and BlastR. Also called pXPR_121

Depositor

John Doench, William Hahn, David Root

Article

Rosenbluh et al Nat Commun. 2017 May 23;8:15403. doi: 10

Insert

dCas9

Use

Lentiviral

Tags

KRAB

Expression

Mammalian

Mutation

dCas9

Promoter

EF1a

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pmStayGold_mStayGold_I3

Plasmid

#239339

Purpose

Expresses the I3-01 nanocage subunit N-terminally tagged with two copies of mStayGold in mammalian cells. Assembles into nanocages tagged with 120 FPs.

Depositor

Torsten Wittmann

Article

Viola et al J Cell Sci. 2025 May 15;138(10):jcs26385

Insert

I3-01

Tags

mStayGold

Expression

Mammalian

Mutation

K129A

Promoter

CMV

Available Since

June 6, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-iCre

Plasmid

#125577

Purpose

Expresses iCre under the CAG promoter in mammalian cells.

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

iCre

Expression

Mammalian

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMET7-GAG-EGFP

Plasmid

#80605

Purpose

Mammalian expression vector that expresses HIV-1 GAG with EGFP fused to it

Depositor

Sven Eyckerman

Article

Eyckerman et al Nat Commun. 2016 Apr 28;7:11416. doi: 10

Insert

EGFP

Expression

Mammalian

Promoter

SRalpha

Available Since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pET28_Cas9d_Plasmid

Plasmid

#239445

Purpose

Expression plasmid codon-optimized for E. coli

Depositor

David Taylor

Article

Ocampo et al Nat Commun. 2025 Jan 7;16(1):457. doi: 1

Insert

Cas9d

Expression

Bacterial

Promoter

T7

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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mPiezo1-IRES-eGFP

Plasmid

#80925

Purpose

expresses mouse Piezo1 with GFP

Depositor

Ardem Patapoutian

Article

Coste et al Science. 2010 Oct 1;330(6000):55-60. doi

Insert

mouse Piezo1 CDS (Piezo1 Mouse)

Expression

Mammalian

Available Since

Nov. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag mPTIP

Plasmid

#15557

Depositor

Kai Ge

Article

Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

Insert

PTIP (Paxip1 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Aug. 16, 2007

Availability

Academic Institutions and Nonprofits only
pAct:dCas9-GCN4

Plasmid

#78899

Purpose

Expresses dCas9-GCN4 under actin promoter for SunTag CRISPRa in Drosophila cell culture

Depositor

Norbert Perrimon

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

dCas9-10XGCN4

Use

CRISPR

Tags

HA

Expression

Insect

Promoter

pActin (Drosophila)

Available Since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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