We narrowed to 14,200 results for: cas9

Showing: 5861 - 5880 of 14200 results

Results per page:

Sort results by:

pMiniT:SEP-GluN1 TKIT donor

Plasmid

#169427

Purpose

Donor DNA for TKIT knockin of SEP to NR1 in mouse

Depositor

Richard Huganir

Publication

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

Super ecliptic pHluorin (SEP)

Use

CRISPR

Expression

Mammalian

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pHFS:SEP-rGluA1 TKIT donor

Plasmid

#169438

Purpose

Donor DNA for TKIT knockin of SEP to GluA1 in rat

Depositor

Richard Huganir

Publication

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

Super ecliptic pHluorin (SEP)

Use

CRISPR

Expression

Mammalian

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMiniT:SEP-GluA1 TKIT donor

Plasmid

#169418

Purpose

Donor DNA for TKIT knockin of SEP to GluA1 in mouse

Depositor

Richard Huganir

Publication

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

Super ecliptic pHluorin (SEP)

Use

CRISPR

Expression

Mammalian

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCB-CBEv2

Plasmid

#221139

Purpose

Coxiella burnetii CRISPR-Cas9 cytosine base editing plasmid version 2 without sgRNA construct. Expresses 3xF-BE4-PpAPOBEC1(H122A).

Depositor

Craig Roy

Publication

Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1

Insert

3xF-PpAPOBEC1(H122A)-Cas9n-UGI-UGI (BE4-PpAPOBEC1(H122A))

Use

CRISPR

Tags

3xFLAG

Expression

Bacterial

Promoter

lacIq-Ptac

Available since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDIV272

Plasmid

#226183

Purpose

Expresses Cas9 and gRNA targeting uidA gene of the pDIV643 derived cassette.

Depositor

Uffe Mortensen

Publication

Strucko et al FEMS Yeast Res. 2024 Aug 23:foae026. doi

Insert

tRNA-sgRNA-tRNA

Use

CRISPR

Expression

Bacterial and Yeast

Promoter

TEF1p (Komagataella phaffii)

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
EC64434

Plasmid

#209518

Purpose

Cloning in complete guide expression cassettes, with Cas9 & Cas12a(D156R)

Depositor

Wendy Harwood, Cristobal Uauy

Publication

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Type

Empty backbone

Use

CRISPR

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CMV-dSa-VPR mini.-1X snRP1

Plasmid

#99687

Purpose

Expresses dSa Cas9 fused to optimized combination of truncated activation domains with 17 nt snRP1 poly adenylation signal

Depositor

George Church

Publication

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

AAV

Tags

VPR mini

Mutation

dead Cas9

Available since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pYZ164

Plasmid

#98408

Purpose

Cas9-gRNA his3 plasmid for his3 deletion in S. pombe

Depositor

Jef Boeke

Publication

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Insert

gRNA targeting Sp.his3 (his3 Fission Yeast)

Use

CRISPR

Available since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
DHFR-PP7-VP64_T2A_GFP

Plasmid

#86167

Purpose

Expresses PP7-VP64 fused to DHFR domain on N-terminus in mammalian cells

Depositor

Amit Choudhary

Publication

Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

Insert

DHFR-PP7-VP64_T2A_GFP

Use

CRISPR and Lentiviral

Tags

Destabilized domain (N-terminus version) of E.col…

Expression

Mammalian

Promoter

EF1a

Available since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
PM-SP!TB

Plasmid

#48650

Purpose

Bacterial SP crRNA expression: targets SP to protospacer B (ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicol

Depositor

George Church

Publication

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial SP crRNA to prototspacer B

Use

CRISPR

Promoter

J23100 promoter

Available since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

Enlarge
pSH624-ssr

Plasmid

#199241

Purpose

Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E

Depositor

Susanne Häußler

Publication

Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

Insert

single-stranded DNA recombineering

Expression

Bacterial

Promoter

lacIq-Ptrc

Available since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
modRNAc0-ABE8e-P2A-GFP

Plasmid

#178177

Purpose

IVT template plasmid for making ABE8e-P2A-GFP modRNA

Depositor

Xiaojun Lian

Publication

Haideri et al Cell Rep Methods. 2022 Sep 7;2(9):100290

Insert

ABE8e

Use

Ivt template plasmid

Tags

P2A-GFP

Promoter

T7 promoter

Available since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
pSF280

Plasmid

#167675

Purpose

Intermediate vector for six gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

Depositor

Niko Geldner

Publication

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

attL1-ccdB-attL2

Use

CRISPR

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
pMH4

Plasmid

#52529

Purpose

serves as PCR-template to generate HR donors for C-terminal 2xFLAG-tag

Depositor

Klaus Foerstemann

Publication

Bottcher et al Nucleic Acids Res. 2014 Apr 19.

Insert

2x FLAG-tag

Use

Blunt cloning kit

Available since

April 14, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
BCJJ163

Plasmid

#117512

Purpose

AtRPS5a promoter Golden Gate Level 0

Depositor

Jonathan D Jones

Publication

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BsaI:GGAG:pRPS5a:AATG:BsaI

Expression

Plant

Promoter

AtRPS5a

Available since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pDD378

Plasmid

#91827

Purpose

Codon-optimized HaloTag donor for the SapTrap cloning system

Depositor

Bob Goldstein

Publication

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

HaloTag-C1

Use

CRISPR

Expression

Worm

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-module1

Plasmid

#222838

Purpose

Subcloning for Golden Gate assembly of CRISPR/Cas9 vectors (module 1)

Depositor

Hidenori Takeuchi

Type

Empty backbone

Use

CRISPR

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-module2

Plasmid

#222839

Purpose

Subcloning for Golden Gate assembly of CRISPR/Cas9 vectors (module 2)

Depositor

Hidenori Takeuchi

Type

Empty backbone

Use

CRISPR

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-module3

Plasmid

#222840

Purpose

Subcloning for Golden Gate assembly of CRISPR/Cas9 vectors (module 3)

Depositor

Hidenori Takeuchi

Type

Empty backbone

Use

CRISPR

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-module4

Plasmid

#222841

Purpose

Subcloning for Golden Gate assembly of CRISPR/Cas9 vectors (module 4)

Depositor

Hidenori Takeuchi

Type

Empty backbone

Use

CRISPR

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge