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We narrowed to 12,952 results for: sequence

Showing: 8821 - 8850 of 12952 results

  2. pFS_1061_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_GGC(BbsI)-Leader-ARRAY2-DR2-FaqI_Term_NotI

    Plasmid
    #184738
    Purpose
    encodes aTc inducible FsRT-Cas1–Cas2 and transcriptionally insulated FsCRISPR Array 2
    Depositor
    Randall Platt
    Article
    Schmidt et al Science. 2022 May 13;376(6594):eabm6038.
    Insert
    FsRT-Cas1(opt)-Cas2(opt)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    sequence codon optimized for expression in E. coli
    Promoter
    pTetA
    Available Since
    May 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    184738-plasmid-map-sequence-id-361531

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  3. pMT-ubb-cytoBirA-2a-mCherry

    Plasmid
    #79885
    Purpose
    Ubiquitin promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein; flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    Use
    Tags
    3x HA
    Expression
    Bacterial
    Mutation
    Promoter
    Ubiquitin
    Available Since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    79885-plasmid-map-sequence-id-150051

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  5. pMT-MCS-cytoBirA-2A-mCherry_Ras

    Plasmid
    #80058
    Purpose
    MCS for cloning promoter to drive HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    Use
    Tags
    3x HA
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80058-plasmid-map-sequence-id-152592

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  6. GAMA-bio

    Plasmid
    #47747
    Purpose
    Expresses enzymatically monobiotinylated full-length GAMA ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised GAMA
    Use
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available Since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47747-plasmid-map-sequence-id-68877

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  9. pBAD mApple

    Plasmid
    #91772
    Purpose
    Bacterial expression of a fluorescent protein, monomeric Apple (mApple). Note: Addgene identified several mutations compared to the final published sequence.
    Depositor
    Erik Rodriguez, Roger Tsien
    Article
    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:
    Insert
    monomeric Apple
    Use
    Tags
    Hexa-Histidine tag, Xpress epitope for detection …
    Expression
    Bacterial
    Mutation
    R97K, E152S, A180S, V207T compared to mApple (FP …
    Promoter
    araBAD
    Available Since
    June 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    91772-plasmid-map-sequence-id-181643

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  11. L-YARS2-9

    Plasmid
    #166533
    Purpose
    scFv of a human scaffold targeting Tyrosyl-tRNA synthetase 2, mitochondrial. Antigen coverage aa 31-477 of 477
    Depositor
    Susanne Gräslund
    Article
    Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Available Since
    April 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    166533-plasmid-map-sequence-id-329205

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  12. pCML 961

    Plasmid
    #98848
    Purpose
    pTriFC_aidB. Expresses protein-NYFP and 2MS2BD-5' UTR mStrawberry fusions in E. coli for Dual-fluorescence TriFC assay (ampR).
    Depositor
    Lydia Contreras
    Article
    Sowa et al Nucleic Acids Res. 2017 Feb 28;45(4):167
    Inserts
    5' UTR + first 100 nucleotides of coding sequence of the aidB gene
    csrA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pLacO
    Available Since
    Nov. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    98848-plasmid-map-sequence-id-194122

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  16. O-MARS-11

    Plasmid
    #166541
    Purpose
    scFv of a human scaffold targeting Methionyl-tRNA synthetase. Antigen coverage aa 1-225 of 900
    Depositor
    Susanne Gräslund
    Article
    Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Available Since
    April 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    166541-plasmid-map-sequence-id-329234

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  18. Hiv7CMV-Cremyc-2A-GFP

    Plasmid
    #117148
    Purpose
    Express Cre enzyme in mammalian cells with floxed genes to achieve somatic knockout. GFP is simultaneously expressed for convenient tracking the transfected cells.
    Depositor
    Qiang Lu
    Article
    Geng et al Nat Commun. 2018 Jul 13;9(1):2707. doi:
    Insert
    Cremyc-2A-GFP
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    May 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    117148-plasmid-map-sequence-id-244039

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  20. pMDC123SB-OsMIR390-B/c

    Plasmid
    #61466
    Purpose
    Plant expression vector (2x35S) for direct cloning of artificial microRNAs into rice MIR390 precursor. To use for expressing amiRNAs in monocots.
    Depositor
    James Carrington
    Article
    Carbonell et al Plant J. 2015 Mar 24. doi: 10.1111/tpj.1
    Insert
    OsMIR390-B/c
    Use
    Tags
    Expression
    Plant
    Mutation
    O. sativa MIR390 precursor sequence including a c…
    Promoter
    2x35S
    Available Since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    61466-plasmid-map-sequence-id-100072

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  22. pFS_1064_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_J23106-Leader-ARRAY2-DR2-FaqI_Term_NotI

    Plasmid
    #184739
    Purpose
    encodes aTc inducible FsRT-Cas1–Cas2 expression cassette and FsCRISPR Array 2 transcribed by BBa_J23106
    Depositor
    Randall Platt
    Article
    Schmidt et al Science. 2022 May 13;376(6594):eabm6038.
    Insert
    FsRT-Cas1(opt)-Cas2(opt)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    sequence codon optimized for expression in E. coli
    Promoter
    pTetA
    Available Since
    May 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    184739-plasmid-map-sequence-id-361530

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  23. p1.1-Tr2-eGFP

    Plasmid
    #162782
    Purpose
    Fluorescent reporter for protein expression studies
    Depositor
    Ivan Vorobiev
    Article
    Sinegubova et al PLoS One. 2021 Feb 2;16(2):e0242890. doi
    Insert
    enhanced green fluorescent protein
    Use
    Tags
    Expression
    Mammalian
    Mutation
    consensus Kozak sequence (GCCGCCATGG) added befor…
    Promoter
    CHO EEF1A1 (Translation Elongation Factor 1 Alpha…
    Available Since
    July 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    162782-plasmid-map-sequence-id-338334

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  28. P41-bio

    Plasmid
    #47739
    Purpose
    Expresses enzymatically monobiotinylated full-length P41 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised P41
    Use
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available Since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47739-plasmid-map-sequence-id-68748

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  30. pFastBacDual with LFn + PrgJ

    Plasmid
    #84869
    Purpose
    Baculovirus Expression of LFn-PrgJ fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel protein
    Depositor
    Russell Vance
    Article
    Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi
    Inserts
    LFn
    PrgJ
    Use
    Baculovirus
    Tags
    6xHis
    Expression
    Insect
    Mutation
    Promoter
    Polyhedrin
    Available Since
    Nov. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    84869-plasmid-map-sequence-id-162908

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Showing: 8821 - 8850 of 12952 results