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hBax S184A C3-EGFP

Plasmid

#19742

Depositor

Richard Youle

Article

Nechushtan et al EMBO J. 1999 May 4. 18(9):2330-41.

Insert

Bax S184A (BAX Human)

Tags

GFP

Expression

Mammalian

Mutation

Serine 184 to Alanine

Available Since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only

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qTAG-C-moxGFP-Zeo

Plasmid

#207732

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a moxGFP-2A-Zeo cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pR562

Plasmid

#207886

Purpose

Creation of Level 0 entry clones

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

amilCP

Expression

Bacterial

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX6p-1-BCCP-PupE

Plasmid

#113403

Purpose

Expresses BCCP-PupE in bacteria

Depositor

Min Zhuang

Article

Liu et al Nat Methods. 2018 Aug 13. pii: 10.1038/s

Insert

BCCP-PupE

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pYFJ84

Plasmid

#227644

Purpose

Vibrio harveyi acyl-ACP synthetase

Depositor

John Cronan

Article

Jiang et al Biochemistry. 2006 Aug 22;45(33):10008-1

Insert

Acyl-Acyl carrier protein synthetase

Tags

10xHis

Expression

Bacterial

Promoter

T7

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pUC19-P70a-Top-T~P70a-Bottom-T

Plasmid

#87314

Purpose

Expresses the modular Split-Broccoli system (Top + Bottom) under the control of E. coli sigma 70 promoters.

Depositor

Donald Burke

Article

Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

Insert

Split-Broccoli system

Use

Synthetic Biology

Expression

Bacterial

Promoter

P70a

Available Since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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GST-mCh-FYVE

Plasmid

#192289

Purpose

Expresses GST- and mCherry-tagged HRS FYVE in e Coli

Depositor

James Hurley

Article

Jensen et al Sci Adv. 2022 Dec 14;8(50):eadd1436. doi

Insert

HRS FYVE domain

Tags

GST-TEV-mCherry

Expression

Bacterial

Promoter

tac

Available Since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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qTAG-KO-Blast

Plasmid

#227263

Purpose

Empty selectable Knock-Out donor cassette. Integrate homology arms to target the N-terminus insertion of a 2A-Blast cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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TOPO mCherry

Plasmid

#68490

Purpose

TOPO construct expressing mCherry for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

mCherry

Use

Gmap

Expression

Bacterial

Promoter

Plac

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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RCASBP-AP (A) (CC#37)

Plasmid

#15160

Depositor

Connie Cepko

Article

Fekete et al Mol Cell Biol. 1993 Apr . 13(4):2604-13.

Insert

PLAP (ALPP Human)

Use

Retroviral; Avian expression

Available Since

June 8, 2007

Availability

Academic Institutions and Nonprofits only

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pEF1α-Cre-PGK-Puro

Plasmid

#207742

Purpose

Co-expression of the Cre recombinase and a puro selectable marker

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

Cre-PGK-Puro

Use

Cre/Lox

Expression

Mammalian

Promoter

EF1alpha

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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Tol2_8xCTS-ECFP

Plasmid

#200249

Purpose

Zebrafish transgenics; Plasmid encodes the sgRNA2_8xCTS-ECFP reporter and could be stably integrated in the zebrafish genome using Tol2 transgenesis

Depositor

Tatjana Sauka-Spengler

Article

Pelea et al bioRxiv 2023.05.08.539738

Insert

8xCTS-ECFP

Use

Zebrafish expression

Available Since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag p38 delta

Plasmid

#20785

Depositor

Roger Davis

Article

Enslen et al EMBO J. 2000 Mar 15. 19(6):1301-11.

Insert

pDCNA3 Flag p38 delta (Mapk13 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

April 14, 2009

Availability

Academic Institutions and Nonprofits only

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pFU-AgaIVA-VG

Plasmid

#24145

Depositor

Ines Ibanez-Tallon

Article

Auer et al Nat Methods. 2010 Mar;7(3):229-36. doi:

Insert

Venus-AgatoxinIVA-FLAG-GPI anchor

Use

Lentiviral

Expression

Mammalian

Available Since

March 24, 2010

Availability

Academic Institutions and Nonprofits only

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pGP-FB-orig BA

Plasmid

#13420

Depositor

Keith Joung

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

orig BA

Tags

Gal11P

Expression

Bacterial

Available Since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only

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pCDH-F-PA1

Plasmid

#40618

Depositor

Kai Ge

Article

Wang et al Proc Natl Acad Sci U S A. 2012 Sep 4.

Insert

PA1 (PAGR1 Human)

Use

Lentiviral

Tags

Flag

Promoter

EF1

Available Since

Oct. 22, 2012

Availability

Academic Institutions and Nonprofits only
CMVTO-GFP-ECL-3TM-tevs-KKMP V3

Plasmid

#179640

Purpose

TEVP-inducible membrane-bound GFP RELEASE construct

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

Membrane-bound GFP RELEASE

Expression

Mammalian

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pT2HLacZpA1I.7 (#59)

Plasmid

#35965

Depositor

Thomas Sato

Article

Schlaeger et al Proc Natl Acad Sci U S A. 1997 Apr 1;94(

Inserts

Tie2 promoter (Tek Mouse)
SDK LacZ
SV40 pA
Tie2 full enhancer

Available Since

July 9, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-cdc2-AF (718)

Plasmid

#39872

Depositor

Jonathon Pines

Article

Hagting et al EMBO J. 1998 Jul 15;17(14):4127-38.

Insert

Cdc2 (CDK1 Human)

Expression

Mammalian

Mutation

AF = T14A, Y15F

Promoter

CMV

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only
pCMU-TGNVHAr

Plasmid

#61178

Purpose

Fluorescent reporter for trans-Golgi network AtVHA-a1-mCherry expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

AtVHA-a1

Tags

mCherry

Expression

Plant

Promoter

AtUBQ10

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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