We narrowed to 6,933 results for: CAD

1. p36-p37-p38-p40-pET-Duet-1-Rfc3-T76D

   Plasmid

   #175051

   Purpose

   Overexpression of human Rfc2,Rfc3-3KtoA,Rfc4,Rfc5 in E.coli

   Depositor

   Brian Kelch

   Article

   Gaubitz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

   Insert

   Replication factor C subunit 5 (RFC2, RFC3, RFC4, RFC5 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changed Thr76 to Asp, phosphomimetic mutation

   Promoter

   T7 promoter

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. p36-p37-p38-p40-pET-Duet-1-Rfc3-3KtoA

   Plasmid

   #175050

   Purpose

   Overexpression of human Rfc2,Rfc3-3KtoA,Rfc4,Rfc5 in E.coli

   Depositor

   Brian Kelch

   Article

   Gaubitz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

   Insert

   Replication factor C subunits 2-5, Rfc3-3KtoA-mutation (RFC4, RFC2, RFC3, RFC5 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOTTC1535 pscAAV mU6 shRNA(scram) CMV-IE Nuc-EYFP

   Plasmid

   #135563

   Purpose

   An AAV vector expressing scrambled shRNA and a nuclear EYFP reporter

   Depositor

   Christopher Richie

   Article

   Venniro et al Proc Natl Acad Sci U S A. 2020 Apr 7;117

   Inserts

   shRNA (scrambled)
   Nuc-EYFP
   

   Use

   AAV and RNAi

   Tags

   3xNLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-IE and mU6

   Available since

   Sept. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pInducer10-SAE1_sh3

   Plasmid

   #168985

   Purpose

   Expresses RFP cDNA and SAE1 shRNA 3 in mammalian cells

   Depositor

   Ji Luo

   Article

   Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   SUMO1 Activating Enzyme Subunit 1 (SAE1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ubc promoter

   Available since

   June 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. MSCVhyg-HA-FLAG_Ubc9_C93A

   Plasmid

   #164937

   Purpose

   Expresses FLAG & HA tagged human Ubiquitin Conjugating Enzyme 9 (UBC9) _C93A mutant cDNA

   Depositor

   Ji Luo

   Article

   Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   human UBC9 (UBE2I Human)
   

   Use

   Retroviral

   Tags

   FLAG & HA tags

   Expression

   Mutation

   Promoter

   PGK promoter

   Available since

   May 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. QuasAr-P2A-hKvb1

   Plasmid

   #154083

   Purpose

   Independent expression of QuasAr and human Kvbeta 1 in pFCK vector

   Depositor

   Michael Hoppa

   Article

   Cho et al Proc Natl Acad Sci U S A. 2020 Nov 24;11

   Insert

   QuasAr-P2A-human Kvb1 (KCNAB1 Human, Synthetic)
   

   Use

   Lentiviral

   Tags

   myc-Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKII

   Available since

   Nov. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBOB-N-3HA-GK_v2

   Plasmid

   #134277

   Purpose

   Lentivector encoding 3XHA-tagged GK (isoform 2)

   Depositor

   Bruce Beutler

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

   Insert

   Gk (Gk Mouse)
   

   Use

   Lentiviral

   Tags

   3X HA

   Expression

   Mammalian

   Mutation

   553 amino acids, isoform 2

   Promoter

   CMV

   Available since

   May 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDM304_YFP-IqgC_mRFP-Raf1(RBD)

   Plasmid

   #128698

   Purpose

   simultaneous expression of YFP-tagged IqgC and mRFP-tagged active Ras probe (GTPase binding domain from Raf1 kinase) in Dictyostelium discoideum cells

   Depositor

   Igor Weber

   Article

   Marinovic et al Proc Natl Acad Sci U S A. 2019 Jan 22;11

   Inserts

   iqgC (full length)
   Raf1 kinase (aa 50-132)
   

   Use

   Dictyostelium discoideum expression vector

   Tags

   YFP and mRFP

   Expression

   Mutation

   none

   Promoter

   Available since

   Aug. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pABD823

   Plasmid

   #67911

   Purpose

   Protein interaction with yeast two hybrid assay of CTG10 in yeast, Saccharomyces cerevisiae

   Depositor

   Bruce Downie

   Article

   Majee et al Proc Natl Acad Sci U S A. 2018 Apr 24;11

   Insert

   Cold temperature germinating 10 (CTG10)
   

   Use

   Tags

   GAL4 DNA binding domain (148 amino acids)

   Expression

   Yeast

   Mutation

   Promoter

   pADH1 (alcohol dehydrogenase 1 promoter)

   Available since

   Dec. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

10. pABD933

    Plasmid

    #67917

    Purpose

    Entry clone made prior to studies of protein co-localization of CTG10 via fluorescent fusion after transient expression in planta

    Depositor

    Bruce Downie

    Article

    Majee et al Proc Natl Acad Sci U S A. 2018 Apr 24;11

    Insert

    CTG10
    

    Use

    Gateway cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3.1_hE2F7mg_6ntmut

    Plasmid

    #73075

    Purpose

    human E2F7 minigene (exons 11,12,13/introns cassette) with mutated snord27 binding site

    Depositor

    Stefan Stamm

    Article

    Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

    Insert

    E2F7 (E2F7 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    partial gene, exons 11,12,13 spaced by introns wi…

    Promoter

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA3.1_hE2F7mg_10ntmut

    Plasmid

    #73076

    Purpose

    human E2F7 minigene (exons 11,12,13/introns cassette) with mutated snord27 binding site

    Depositor

    Stefan Stamm

    Article

    Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

    Insert

    E2F7 (E2F7 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    partial gene, exons 11,12,13 spaced by introns wi…

    Promoter

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. N295A (EcN), E166Q (EcN) MetNI LmH

    Plasmid

    #118269

    Purpose

    N295A and E166Q Mutation of MetN in Methionine ABC Transporter with 6x His Tag. Used to solve crystal structure

    Depositor

    Douglas Rees

    Article

    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p

    Inserts

    MetI (metI Escherichia coli)
    MetN (E166Q ; N295A) (metN Escherichia coli)
    

    Use

    Tags

    6x His Tag

    Expression

    Bacterial

    Mutation

    Changed Glutamic Acid (Glu) 166 to Glutamine (Gln…

    Promoter

    T7 Promoter

    Available since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. Met1 diUb (1-152, R42W)

    Plasmid

    #110767

    Purpose

    Bacterial expression of Met1 diUb

    Depositor

    David Komander

    Article

    Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

    Insert

    UBB (UBB Human)
    

    Use

    Tags

    N-His6-3C

    Expression

    Bacterial

    Mutation

    R42W

    Promoter

    T7

    Available since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAP1893-1

    Plasmid

    #105248

    Purpose

    human GFP11 with tagRFP 33bp downstream in Lamin A/C (recoded)

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Proc Natl Acad Sci U S A. 2017 Nov 28. p

    Insert

    Insertion of GFP11 at cut tagRFP 33bp downstream (recoded *) in Lamin A/C (LMNA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. Adeno BN

    Plasmid

    #101823

    Purpose

    Adenovirus for the expression of gRNAs targeting intron 13 of murine Bcan and intron 10 of murine Ntrk1

    Depositor

    Andrea Ventura

    Article

    Cook et al Nat Commun. 2017 Jul 11;8:15987. doi: 10

    Insert

    U6_sgRNA(Bcan)_U6_sgRNA(Ntrk1)_CBh_FLAG-Cas9 (Bcan, Ntrk1 Mouse)
    

    Use

    Adenoviral

    Tags

    FLAG-Cas9

    Expression

    Mutation

    Promoter

    Available since

    Nov. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

17. pCOG40-HRS (257-276) Hs

    Plasmid

    #83236

    Purpose

    pGST-HRS (257-276) Hs, Expresses the GST-HRS in E.coli

    Depositor

    Gali Prag

    Article

    Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

    Insert

    HRS (257-276) Hs (HGS Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    258-277

    Promoter

    Available since

    Nov. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. tdEos-EB3-7

    Plasmid

    #57610

    Purpose

    Localization: MT End Binding Protein, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

    Insert

    EB3 (MAPRE3 Human)
    

    Use

    Tags

    tdEos

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 16, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

19. pET21a-MKP7_K62R_153

    Plasmid

    #37795

    Depositor

    SeWon Suh

    Article

    Kim et al Proc Natl Acad Sci U S A. 2012 May 15;10

    Insert

    Mitogen-activated protein kinase phosphatase 7 (DUSP16 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    changed Lysnie 62 to Arginine and deleted amino a…

    Promoter

    Available since

    Sept. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pET21a-MKP7_K52R_153

    Plasmid

    #37793

    Depositor

    SeWon Suh

    Article

    Kim et al Proc Natl Acad Sci U S A. 2012 May 15;10

    Insert

    Mitogen-activated protein kinase phosphatase 7 (DUSP16 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    changed Lysnie 52 to Arginine and deleted amino a…

    Promoter

    Available since

    Sept. 27, 2012

    Availability

    Academic Institutions and Nonprofits only