We narrowed to 34,404 results for: CaS

1. pEn-C1.1

   Plasmid

   #61479

   Purpose

   Encodes CRISPR sgRNA for Gateway or conventional cloning of 1-3 sgRNAs into pDe-CAS9 or pCAS9-TPC

   Depositor

   Holger Puchta

   Article

   Schiml et al Plant J. 2014 Oct 18. doi: 10.1111/tpj.1

   Insert

   AtU6-26:sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   U6-26

   Available since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSL0426 (CRISPR with spc3)

   Plasmid

   #130642

   Purpose

   Expresses CRISPR RNA from a T7 promoter for VchCAST system. The CRISPR array encodes a guide RNA with spacer 3 from the native CRISPR.

   Depositor

   Samuel H. Sternberg

   Article

   Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

   Insert

   CRISPR(spacer-3)
   

   Use

   CRISPR; Transposon

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pG3B-U6SC

   Plasmid

   #134753

   Purpose

   pGreen3 CRISPR/Cas9

   Depositor

   Qi-Jun Chen

   Article

   Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

   Insert

   CRISPR/Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   gRNA-U6, zCas9-35SC

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. p2T-CMV-BE4max-BlastR

   Plasmid

   #152991

   Purpose

   C-to-T base editor

   Depositor

   David Liu

   Article

   Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

   Insert

   BE4-nSpCas9-UGI-UGI
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEPOR1CB0013

   Plasmid

   #117549

   Purpose

   Level 1 Golden Gate Cassette: LbCas12a expression cassette for dicotyledonous plants

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   2x35S+5'UTR OMEGA (pICH51288) + LbCas12a (with stop codon) (pEPOR0SP0007)+ Nos (pICH41421)
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   2x35S+5'UTR OMEGA (pICH51288)

   Available since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pG3B-U3Ub

   Plasmid

   #134750

   Purpose

   pGreen3 CRISPR/Cas9

   Depositor

   Qi-Jun Chen

   Article

   Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

   Insert

   CRISPR/Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   gRNA- OsU3, zCas9-Ubi1

   Available since

   Dec. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFYF1327 EGFP Site#2

   Plasmid

   #47512

   Purpose

   human gRNA expression vector targeting EGFP

   Depositor

   Keith Joung

   Article

   Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

   Insert

   gRNA-EGFP site 2
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. Oct4-Cr1

   Plasmid

   #66989

   Purpose

   Expresses gRNA that targets stop codon for OCT4

   Depositor

   Danwei Huangfu

   Article

   Zhu et al Stem Cell Reports. 2015 May 28. pii: S22

   Insert

   gRNA for Crispr targeting the OCT4 stop codon
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 22, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pJK503

   Plasmid

   #155380

   Purpose

   dCas12a oscillator, same direction crRNAs. dCas12a (D917A) + PA4-mVenus

   Depositor

   Pamela Silver

   Article

   Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

   Inserts

   dCas12a (F. novicida)
   PA4-mVenus
   dCas12a oscillator crRNAs
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   D917A (nuclease-deactivating)

   Promoter

   Available since

   May 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. EFS eGFP hPGK BSD

    Plasmid

    #188520

    Purpose

    Backbone for CRISPR interference (CRISPRi) isogenic PAM testing

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-calx1

    Plasmid

    #58697

    Purpose

    Expression of calx1

    Depositor

    Craig Montell

    Article

    Wang et al Neuron 2005 Feb 3;45(3):367-78.

    Insert

    calx1 (Calx Fly)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 21, 2014

    Availability

    Academic Institutions and Nonprofits only

12. p2T-CMV-evoAmax-BlastR

    Plasmid

    #152996

    Purpose

    C-to-T base editor

    Depositor

    David Liu

    Article

    Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

    Insert

    evoA-nSpCas9-UGI-UGI
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTol2-HybridBarcode

    Plasmid

    #174037

    Purpose

    For Tol2 transgenesis of expressed barcode containing SpyCas9, SauCas9, and LbCas12a target sites

    Depositor

    James Gagnon

    Article

    Takasugi et al Genetics. 2021 Nov 4. pii: 6420709. doi:

    Insert

    CRISPR target sites
    

    Use

    Tol2 transgenesis

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pG3H-U6EC1

    Plasmid

    #134758

    Purpose

    pGreen3 CRISPR/Cas9

    Depositor

    Qi-Jun Chen

    Article

    Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

    Insert

    CRISPR/Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    gRNA-U6, zCas9-EC1

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pICSL11023

    Plasmid

    #117542

    Purpose

    Level 1 Golden Gate Cassette: Cas9 expression cassette for dicotyledonous plants

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    2x35S+5'UTR OMEGA (pICH51288) + SpCas9-h (no stop codon)(pICSL90005) + YFP (pICSL50005)+ Nos (pICH41421)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    2x35S_OMEGA(pICH51288)

    Available since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKDsgRNA-ldhA

    Plasmid

    #102288

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting ldhA.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    ldhA gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available since

    April 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKDsgRNA-rpsL

    Plasmid

    #89953

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting rpsL.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    rpsL gRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJEC626

    Plasmid

    #174367

    Purpose

    Activation domain for modular CRISPRa. AD-SYNZIP. Expresses alphaNTD from the E coli RNAP with a SYNZIP17 domain fused to the N terminus.

    Depositor

    James Chappell

    Article

    Villegas Kcam et al Nucleic Acids Res. 2021 May 7;49(8):4793

    Insert

    Alpha N-terminal domain from the E. coli RNA polymerase fused to SYNZIP17
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23106

    Available since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. Zebrafish-gRNA-0007

    Plasmid

    #42247

    Purpose

    gRNA targeted to zebrafish gene th1

    Depositor

    Keith Joung

    Article

    Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

    Insert

    gRNA-th1 (th Zebrafish)
    

    Use

    CRISPR; Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Jan. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pG3K-U6EC1

    Plasmid

    #134757

    Purpose

    pGreen3 CRISPR/Cas9

    Depositor

    Qi-Jun Chen

    Article

    Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

    Insert

    CRISPR/Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    gRNA-U6, zCas9-EC1

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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