Purpose
E. coli plasmid encoding a fusion between pH-Countdown and the calcium-binding domains of GECO 1.2 with a hexahistidine tag, targeted to the periplasm via the TorA presequence.
Insert
pH-Countdown insertion into GECO 1.2 calcium sensing domains
Tags
A modified TorA periplasmic targeting sequence pl…
Mutation
Spontaneous mutation of calmodulin's penulti…
Available Since
April 28, 2022
Availability
Academic Institutions and Nonprofits only