We narrowed to 17,102 results for: EMB

1. pDD382

   Plasmid

   #91830

   Purpose

   Lox2272-flanked SEC donor for the SapTrap cloning system

   Depositor

   Bob Goldstein

   Article

   Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

   Insert

   Lox2272-SEC-Lox2272
   

   Use

   CRISPR and Cre/Lox

   Expression

   Worm

   Available Since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. qTAG-C-mNeon-Zeo

   Plasmid

   #207731

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mNeon-2A-Zeo cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. qTAG-C-miRFP670nano3-Puro

   Plasmid

   #207724

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a miRFP670nano3-2A-Puro cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. qTAG-N-Zeo-mNeon

   Plasmid

   #207698

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-mNeon cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. qTAG-N-Puro-V5

   Plasmid

   #207697

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-V5 cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. YIplac211-CHS3-msGFPx3

   Plasmid

   #105257

   Purpose

   Plasmid for pop-in/pop-out replacement of Chs3 with Chs3-msGFPx3.

   Depositor

   Benjamin Glick

   Article

   Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

   Insert

   Chs3 portion (CHS3 Budding Yeast)
   

   Tags

   msGFPx3

   Expression

   Yeast

   Available Since

   Feb. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET_RFP-SZ2

   Plasmid

   #124933

   Purpose

   Expression of RFP-SZ2

   Depositor

   Matthew Good, Daniel Hammer, Benjamin Schuster

   Article

   Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

   Insert

   RFP-SYNZIP2
   

   Tags

   His and mCherry

   Expression

   Bacterial

   Mutation

   SZ2 domain

   Available Since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEF-mRuby3

   Plasmid

   #179730

   Purpose

   Mammalian expression of mRuby3 from the EF1 promoter

   Depositor

   Edwin Chapman

   Article

   Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

   Insert

   mRuby3
   

   Expression

   Mammalian

   Available Since

   March 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. Gata4-GFP donor

   Plasmid

   #113115

   Purpose

   Donor plasmid for knock-in eGFP fusing to the c-terminal of mouse Gata4 coding sequence

   Depositor

   Janet Rossant

   Article

   Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

   Insert

   Gata4 donor region including homology arms and eGFP
   

   Use

   Synthetic Biology

   Available Since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMaCTag-07

    Plasmid

    #124786

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (without selection) with mNeonGreen

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mNeonGreen
    

    Use

    Pcr template

    Tags

    mNeonGreen

    Promoter

    None

    Available Since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRSET-His-APEX2

    Plasmid

    #201471

    Purpose

    Recombinant soluble APEX2 for SLAPSHOT labeling

    Depositor

    Yuh-Nung Jan

    Article

    Tuomivaara et al Commun Biol. 2024 Aug 29;7(1):1060. doi:

    Insert

    APEX2
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTn7-SCOUT21.RY

    Plasmid

    #201006

    Purpose

    pLv1-pUC18R6KT-miniTn7-Gm-mCherry-sYFP2, GmR, ApR

    Depositor

    Philip Poole

    Article

    Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

    Inserts

    mcherry
    sYFP2
    

    Expression

    Bacterial

    Promoter

    J23104

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTn7-SCOUT21.YB

    Plasmid

    #201008

    Purpose

    pLv1-pUC18R6KT-miniTn7-Gm-sYFP2-TagBFP, GmR, ApR

    Depositor

    Philip Poole

    Article

    Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

    Inserts

    sYFP2
    TagBFP
    

    Expression

    Bacterial

    Promoter

    J23104

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFBNb-cALFA

    Plasmid

    #159990

    Purpose

    For the protein expression with ALFA tag at C-terminus in insect cells

    Depositor

    Motoyuki Hattori

    Article

    Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

    Type

    Empty backbone

    Tags

    HRV3C protease cleavage site-ALFA tag-8histidine

    Expression

    Insect

    Available Since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. qTAG-N-Zeo-V5

    Plasmid

    #207708

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-V5 cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pVENUS-N1-POLR2G-GFP

    Plasmid

    #86872

    Purpose

    To express POLR2G (RPB7) tagged with VENUS at C terminus.

    Depositor

    Yihong Ye

    Article

    Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

    Insert

    POLR2G (POLR2G Human)
    

    Tags

    VENUS

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDL1

    Plasmid

    #182263

    Purpose

    Gibson cloning vector for synthesis of single and double stranded RNA

    Depositor

    Daniel Lobo

    Article

    Wolff et al STAR Protoc. 2022 Jun 14;3(3):101458. do

    Type

    Empty backbone

    Use

    Gibson cloning vector for synthesis of single and…

    Promoter

    T3 and SP6 for sense and antisense riboprobe synt…

    Available Since

    June 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-cdc2 (716)

    Plasmid

    #61840

    Purpose

    mammalian expression of cdc2

    Depositor

    Jonathon Pines

    Article

    Hagting et al EMBO J. 1998 Jul 15;17(14):4127-38.

    Insert

    cdc2 (CDK1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

19. PlayBack-Ef1a-Bamh1

    Plasmid

    #203308

    Purpose

    For expression of eukaryotic genes of interest with Ef1a promoter with Bamh1 as a PlayBack Restriction Enzyme

    Depositor

    Aleksandar Necakov

    Article

    Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. TagBFP2-PIP4K2C

    Plasmid

    #202730

    Purpose

    expresses fluorescent protein-tagged enzyme

    Depositor

    Gerry Hammond

    Article

    Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

    Insert

    mTagBFP2:PIP4K2C (PIP4K2C Human)
    

    Expression

    Mammalian

    Available Since

    June 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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