We narrowed to 7,184 results for: RAP

1. pHIV-BRAFV600E-mOrange2

   Plasmid

   #110732

   Purpose

   Mammalian Expression of BRAFV600E IRES H2B-mOrange2

   Depositor

   Robert Judson-Torres

   Article

   Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

   Insert

   BRAFV600E IRES H2B-mOrange2 (BRAF Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   BRAF V600E

   Promoter

   Available since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOTTC1484 - pAAV SYN1 HA-hM4D(Gi)

   Plasmid

   #121538

   Purpose

   An adeno-associated viral vector expressing HA-tagged inhibitory DREADD (hM4D(Gi)) from a synapsin promoter

   Depositor

   Christopher Richie

   Article

   Galvan et al Eur J Neurosci. 2019 May 7. doi: 10.1111

   Has Service

   AAV2 and AAV5

   Insert

   HA-tagged hM4D(Gi) (CHRM4 Human)
   

   Use

   AAV

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   SYN1

   Available since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pIG-601_NY-ESO-1_TCR_GFP

   Plasmid

   #207482

   Purpose

   This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

   Depositor

   Alexander Marson

   Article

   Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

   Insert

   GFP, NY-ESO-1_TCR
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Potential silent point mutation in GFP sequence

   Promoter

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. METTL3 shRNA 2 tet pLKO puro

   Plasmid

   #162984

   Purpose

   Tet-inducible shRNA targeting human METTL3 #2

   Depositor

   Mark Rubin

   Article

   Cotter et al Mol Cancer Res. 2021 Jun 4. pii: 1541-77

   Insert

   methyltransferase like 3 (METTL3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-imp α

   Plasmid

   #119718

   Purpose

   Expresses EGFP-tagged human importinα in mammalian cells

   Depositor

   Kenji Sugimoto

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2012;76(2):38

   Insert

   importinα (KPNA2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   contains amino acids 251-529

   Promoter

   CMV

   Available since

   Sept. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPMQAK1-Ptrc-[E*](104)-Cas9-B0015-lacZ-PnrsB-mazF-LVA

   Plasmid

   #190790

   Purpose

   [E*](104) construct. Theophylline inducible Cas9 (S. pyogenes) expression. Two BsaI-sites allow for simultaneous insertion of sgRNA and donor DNA. Includes a nickel-inducible curing system.

   Depositor

   Paul Hudson

   Article

   Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102

   Inserts

   Cas9
   mazF-LVA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   LVA

   Expression

   Bacterial

   Mutation

   silent mutations of BpiI-sites in original Cas9 g…

   Promoter

   PnrsB and Ptrc-[E*](104)

   Available since

   Feb. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLY098-RetroEFS-BCMABBz-WPRE

   Plasmid

   #192199

   Purpose

   Retro-BCMACAR

   Depositor

   Sidi Chen

   Article

   Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

   Insert

   Retro-BCMACAR (TNFRSF17 Synthetic)
   

   Use

   Retroviral; Mammalian expression

   Tags

   Expression

   Mutation

   NA

   Promoter

   Available since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHLS-EF1a-FKBP12-Gag(HIV)

   Plasmid

   #138476

   Purpose

   Expresses FKBP12 fused Gag for packaging FRB-SpCas9 into NanoMEDIC particle.

   Depositor

   Akitsu Hotta

   Article

   Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

   Insert

   HIV Gag
   

   Use

   Tags

   FRBP12 and Lynn Myristolation site

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pXD024 - pLKO-EF1a-GFP-P2A-FLuc-WPRE

   Plasmid

   #192204

   Purpose

   Lenti-GL(GFP-Luciferase)

   Depositor

   Sidi Chen

   Article

   Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

   Insert

   Lenti-GL(GFP-Luciferase)
   

   Use

   Lentiviral; Mammalian expression

   Tags

   Expression

   Mutation

   NA

   Promoter

   Available since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRC54-DHX37-GFP

    Plasmid

    #192149

    Purpose

    express DHX37-GFP

    Depositor

    Sidi Chen

    Article

    Dong et al Cell. 2019 Aug 22;178(5):1189-1204.e23.

    Insert

    DHX37-GFP (Dhx37 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    Nov. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. Pet22b-YAP

    Plasmid

    #166443

    Purpose

    Bacterial expression of 6x His tagged YAP

    Depositor

    Shuguo Sun

    Article

    Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

    Insert

    YAP (YAP1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOTTC1596 - pAAV SYN1 HA-hM3D(Gq)

    Plasmid

    #121539

    Purpose

    An adeno-associated viral vector expressing HA-tagged stimulatory DREADD (hM3D(Gq)) from a synapsin promoter

    Depositor

    Christopher Richie

    Article

    Galvan et al Eur J Neurosci. 2019 May 7. doi: 10.1111

    Has Service

    AAV Retrograde, AAV2, and AAV5

    Insert

    HA-tagged hM3D(Gq) (CHRM3 Human)
    

    Use

    AAV

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    SYN1

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-PPO-mScarlet

    Plasmid

    #139503

    Purpose

    Expresses mScarlet-tagged parapinopsin in mammalian cells for photoswitchable control of inhibitory GPCR signaling cascades.

    Depositor

    Michael Bruchas

    Article

    Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

    Insert

    parapinopsin
    

    Use

    Tags

    mScarlet following Gly-Gly-Ser linker

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. PL-5LTR-RGR(DMD​#​1​)-AmCyan-A

    Plasmid

    #138482

    Purpose

    Expresses sgRNA targeting human DMD (Dystrophin) for packaging into NanoMEDIC particle.

    Depositor

    Akitsu Hotta

    Article

    Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

    Insert

    Ribozyme-flanked gRNA and AmCyan
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEMHE-membrane-mEGFP

    Plasmid

    #105526

    Purpose

    T7 promotor drives in vitro mRNA transcription of a mEGFP-tagged membrane reporter

    Depositor

    Melina Schuh

    Article

    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

    Insert

    GNAI2 (GNAI2 Human)
    

    Use

    In vitro transcription

    Tags

    mEGFP

    Expression

    Mutation

    fragment encoding the plasma membrane targeting s…

    Promoter

    T7

    Available since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAG426GAL-SLEV_NS2B-GS-NS3

    Plasmid

    #203510

    Purpose

    Expresses SLEV NS2B-NS3 protease (with GS linker) from a GAL promoter with a URA3 marker

    Depositor

    Alejandro Chavez

    Article

    Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

    Insert

    poly SLEV NS2B-GS-NS3 (POLY )
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    July 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDEST-CMV mCherry-GFP-LC3B G120A

    Plasmid

    #123235

    Purpose

    Expresses mCherry-EGFP-LC3B G120A in mammalian cells. Negative control for tandem reporter. High expression driven by CMV promoter.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    

    Use

    Tags

    EGFP and mCherry

    Expression

    Mammalian

    Mutation

    Glycine 120 to Alanine

    Promoter

    CMV

    Available since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti PGK Puro GFP-LC3B G120

    Plasmid

    #123241

    Purpose

    Expression vector with PGK promoter for low expression of EGFP-LC3B G120. For lentivirus production and stable transduction in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Deleted amino acids 121-125. Stop codon after G12…

    Promoter

    PGK

    Available since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. LentiCRISPRv2-sgCTRL

    Plasmid

    #209750

    Purpose

    Lentiviral transfer plasmid to express Cas9 and a control, non-specific gRNA (does not target any human gene)

    Depositor

    Andrei Thomas-Tikhonenko

    Article

    Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

    Insert

    Cas9
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. mEGFP-N1-YAP

    Plasmid

    #166457

    Purpose

    Expresses fusion of mEGFP and YAP

    Depositor

    Shuguo Sun

    Article

    Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

    Insert

    mEGFP, YAP (YAP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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