163,682 results

1. pCAG PSAM4 GlyR IRES EGFP

   Plasmid

   #119739

   Purpose

   Chemogenetic inhibitor expression plasmid

   Depositor

   Scott Sternson

   Article

   Magnus et al Science. 2019 Mar 14. pii: science.aav52

   Insert

   PSAM4 GlyR IRES eGFP (CHRNA7 Human, Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   L131G, Q139L, Y217F

   Promoter

   CAG

   Available Since

   March 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. PCR4-Hsp68::lacZ-H11

   Plasmid

   #139099

   Purpose

   enSERT LacZ reporter vector for site-specific integration into the H11 locus (contains Hsp68 minimal promoter)

   Depositor

   Len Pennacchio

   Article

   Kvon et al Cell. 2020 Mar 6. pii: S0092-8674(20)302

   Type

   Empty backbone

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Hsp68

   Available Since

   March 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCI flag PCAF

   Plasmid

   #8941

   Depositor

   Yoshihiro Nakatani

   Article

   Yang et al Nature. 1996 Jul 25. 382(6589):319-24.

   Insert

   PCAF (KAT2B Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 14, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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4. pZFN-AAVS1_ELD

   Plasmid

   #159297

   Purpose

   ZFN (left) targeting the AAVS1 locus with obligate heterodimer FokI

   Depositor

   Kosuke Yusa

   Insert

   ZFN targeting the AAVS1 locus
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available Since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-28a (+)-hTBP

   Plasmid

   #171073

   Purpose

   Expresses human TBP in bacteria. The resulting plasmid was used to generate a single expression construct encoding a His-tagged TBP

   Depositor

   Joan Conaway

   Article

   He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

   Insert

   TATA-box-binding protein (TBP Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available Since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-p150Glued

   Plasmid

   #36154

   Depositor

   David Stephens

   Article

   Watson et al J Cell Sci. 2006 Jul 1;119(Pt 13):2758-6

   Insert

   p150Glued (DCTN1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   see documentation

   Promoter

   Available Since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pci-EGFP-UPF1-WT

   Plasmid

   #135994

   Purpose

   WT UPF1 inserted with GFP tagged on the N-terminus used to stably integrate into cells

   Depositor

   Anthony Leung

   Article

   Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

   Insert

   UPF1
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-Hs.cargo(Cas9)

   Plasmid

   #174864

   Purpose

   Expresses human Peg10 cargoRNA encoding SpCas9

   Depositor

   Feng Zhang

   Article

   Segel et al Science. 2021 Aug 20;373(6557):882-889.

   Insert

   SpCas9 flanked by HsPeg10 UTRs
   

   Use

   Tags

   HA-SV40 NLS and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJJB308

   Plasmid

   #107699

   Purpose

   AtU6 Promoter-LwaCas13a-gRNA Scaffold

   Depositor

   Daniel Voytas

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGFP-hPMLIII

    Plasmid

    #181903

    Purpose

    Fluorescently tagged PMLIII

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    hPMLIII (PML Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lck-Halo_pTwistCMVBetaGlobWPRENeo

    Plasmid

    #221900

    Purpose

    Expresses Halo7 targeted to the plasma membrane

    Depositor

    Janelia Integrative Imaging

    Insert

    Lck-Halo7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMDH

    Plasmid

    #133129

    Purpose

    A recombinant plasmid expressing mdh (malate dehydrogenase) gene

    Depositor

    Gregg Beckham

    Article

    Guarnieri et al Appl Environ Microbiol. 2017 Aug 17;83(1

    Insert

    malate dehydrogenase gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    malate dehydrogenase promoter

    Available Since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pZac2.1 gfaABC1D-lck-GCaMP6f

    Plasmid

    #52924

    Purpose

    Fluorescent reporter for calcium signaling

    Depositor

    Baljit Khakh

    Has Service

    AAV5

    Insert

    lck-GCaMP6f
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mutation

    Promoter

    Available Since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSR43.6r

    Plasmid

    #63197

    Purpose

    Expresses CcaS, Ho1, and PcyA constitutively

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

    Inserts

    CcaS
    Ho1
    PcyA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. V5-GFP-TurboID-KDEL_pLX208

    Plasmid

    #202020

    Purpose

    expresses GFP-TurboID in the mammalian ER lumen, lentiviral vector

    Depositor

    Alice Ting

    Article

    Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

    Insert

    V5-TurboID-KDEL
    

    Use

    Lentiviral

    Tags

    GFP and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUb PspCas13b + PspCas13b guide RNA

    Plasmid

    #176305

    Purpose

    Expresses PspCas13b and its associated guide RNA in Drosophila cells

    Depositor

    Jeremy Wilusz

    Article

    Ai et al Nucleic Acids Res. 2022 Mar 4. pii: 6542

    Insert

    PspCas13b
    

    Use

    Tags

    HA tag

    Expression

    Insect

    Mutation

    E113K, N265S- please depositor comments

    Promoter

    Ubi-p63e

    Available Since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMKV057

    Plasmid

    #133312

    Purpose

    BeYDV viral replicon on T-DNA backbone expressing Firefly Luc+, WUS2 and IPT

    Depositor

    Daniel Voytas

    Article

    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Insert

    Luc+. WUS2, IPT
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtUbi10, Nos, 35S

    Available Since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLT 651

    Plasmid

    #59998

    Purpose

    Expresses a hybrid dsRNA corresponding to the Caenorhabditis elegans klp-16/him-8 genes in bacteria; ingestion of the bacteria by C elegans produces an RNAi response & leads to more male progeny

    Depositor

    Lisa Timmons

    Article

    Timmons et al FEBS Lett. 2014 Jul 24. pii: S0014-5793(

    Inserts

    klp-16 (klp-16 Nematode)
    him-8 (him-8 Nematode)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    partial genomic

    Promoter

    Available Since

    April 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV.TBG.PI.Null.bGH

    Plasmid

    #105536

    Purpose

    empty AAV vector with TBG promoter

    Depositor

    James M. Wilson

    Has Service

    AAV8

    Insert

    None
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TBG

    Available Since

    Feb. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFUS-WT-VC

    Plasmid

    #175068

    Purpose

    expresses FUS wildtype tagged to mVenus and mCherry in mammalian cells

    Depositor

    Arnold Boersma

    Article

    Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018

    Insert

    FUS glycine rich protein (FUS Human)
    

    Use

    Tags

    HA and mVenus + mCherry

    Expression

    Mammalian

    Mutation

    mVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…

    Promoter

    CMV Promoter

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. pmCherry-NLS

    Plasmid

    #39319

    Depositor

    Martin Offterdinger

    Article

    Micutkova et al Int J Cancer. 2012 Apr 1;130(7):1544-57.

    Insert

    mCherry-3xNLS
    

    Use

    Tags

    3xSV40LT NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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22. pICE-HA-MRE11-WT

    Plasmid

    #82033

    Purpose

    Plasmid for constitutive or doxycycline-inducible expression of wild-type human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

    Depositor

    Sébastien Britton, Patrick Calsou

    Article

    Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

    Insert

    MRE11 (MRE11 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-tet

    Available Since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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23. hMEF2A WT

    Plasmid

    #118354

    Purpose

    This plasmid expresses human MEF2A-1 WT.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

    Insert

    hMEF2A (MEF2A Human)
    

    Use

    Tags

    myc and 6xHIS

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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24. qTAG-C-mStayGold-EFS-Blast

    Plasmid

    #227257

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-EFS-Blast cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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25. psgRNA

    Plasmid

    #114005

    Purpose

    express sgRNA. ColE1, Kan

    Depositor

    David Bikard

    Article

    Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

    Insert

    gRNA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    BBa_J23119

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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26. PB-TO-MYOD1-shOct4

    Plasmid

    #182309

    Purpose

    Piggybac Tet-ON plasmid for differentiating hiPSCs into skeletal muscle cells via MYOD1 and shOCT4 expression

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Inserts

    MYOD1-shOct4 (MYOD1 Human)
    puro-T2A-mycNLS-mTagBFP2
    

    Use

    Tags

    T2A-mycNLS-mTagBFP2

    Expression

    Mammalian

    Mutation

    shOct4

    Promoter

    EF1a and TRE3G

    Available Since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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27. qTAG-C-3xHA-Puro

    Plasmid

    #207729

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xHA-2A-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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28. Fuw-dCas9-dead Tet1CD-P2A-BFP

    Plasmid

    #108246

    Purpose

    Lentiviral vector to express dCas9-dead Tet1CD-P2A-tagBFP

    Depositor

    Rudolf Jaenisch

    Article

    Liu et al Cell. 2018 Feb 8. pii: S0092-8674(18)300

    Insert

    dCas9-dead Tet1CD-HA-P2A-BFP
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    dCas9-D10A, dCas9-H840A, Tet1CD-H1672Y, Tet1CD-D1…

    Promoter

    Ubc

    Available Since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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29. cytoplasmic EKAR (Cerulean-Venus)

    Plasmid

    #18679

    Depositor

    Karel Svoboda

    Article

    Harvey et al PNAS 2008 Dec 9;105(49):19264-9

    Insert

    ERK Activity Reporter
    

    Use

    Tags

    monomeric Cerulean and monomeric Venus

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 2, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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30. MIGR1

    Plasmid

    #27490

    Depositor

    Warren Pear

    Article

    Pear et al Blood. 1998 Nov 15. 92(10):3780-92.

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    GFP and IRES

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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