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pGTag-eGFP-CAAX-SV40

Plasmid

#117815

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

eGFP

Use

Synthetic Biology

Tags

CAAX

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pGTag-NLS-TagRFP-SV40

Plasmid

#117805

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

TagRFP

Use

Synthetic Biology

Tags

NLS

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-(BbsI)sgRNA_CAG-Cas9-bpA_EF1-TagRFP

Plasmid

#86987

Purpose

For cloning and expression of sgRNA together with expression of Cas9 and TagRFP

Depositor

Ralf Kuehn

Article

Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

Insert

Cas9

Expression

Mammalian

Promoter

CAG

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pS1643 (pFA6-NATMX-CDC42pr-yGFP1-10)

Plasmid

#86420

Purpose

N-terminal PCR tagging cassette for yeast codon optimized split GFP1-10 with CDC42 promoter

Depositor

Sue Jaspersen

Article

Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

Insert

CDC42pr-GFP1-10

Tags

GFP1-10

Expression

Yeast

Available Since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pAP24

Plasmid

#70190

Purpose

Modular vector for secreted luciferase reporter expression

Depositor

Wiep Klaas Smits

Article

Oliveira Paiva et al ACS Synth Biol. 2016 Jun 23.

Insert

sLucopt

Use

Synthetic Biology

Expression

Bacterial

Promoter

Ptet

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only

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pCY 3110-01

Plasmid

#36236

Depositor

Anne Robinson

Article

Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

Insert

mEos2

Use

Yeast cassette for pcr and homologous recombinati…

Tags

yEpolylinker-mEos2-kanMX

Available Since

June 19, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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pPIGA-PHD

Plasmid

#26778

Depositor

Linzhao Cheng

Article

Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

Insert

PGK-Hyg-p(A) (PGK1 )

Expression

Mammalian

Available Since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only

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pGL3-PM-34a-K1/2

Plasmid

#25800

Depositor

Judy Lieberman

Article

Navarro et al Blood. 2009 Sep 3. 114(10):2181-92.

Insert

alternative hsa-miR-34a promoter

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

Aug. 9, 2010

Availability

Academic Institutions and Nonprofits only
pHR_TRE_Ecad

Plasmid

#133805

Purpose

inducible expression of Ecad in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2018 Jul 13;361(6398):156-162.

Insert

Homo sapiens cadherin 1 (CDH1) (CDH1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-INSR-V5/HIS

Plasmid

#201980

Purpose

expression of human INSR receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

INSR receptor tyrosine kinase (INSR Human)

Tags

V5, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-IRES-Gαi*-BERKY3

Plasmid

#158424

Purpose

BRET biosensor for detection of Gαi-GTP in lentiviral vector pLVX-IRES-Hyg

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

Lyn11-Nluc-ER/K linker-3xYFP-KB1753-myc

Use

Lentiviral

Tags

myc

Promoter

CMV

Available Since

Sept. 17, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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PGL3-NOXA-N2

Plasmid

#26113

Depositor

Yihong Ye

Article

Wang et al Proc Natl Acad Sci U S A. 2009 Feb 17. 1

Insert

NOXA N2 (PMAIP1 Human)

Use

Luciferase

Expression

Mammalian

Available Since

Sept. 23, 2010

Availability

Academic Institutions and Nonprofits only
pBabe puro MTH1

Plasmid

#21295

Depositor

Bob Weinberg

Article

Rai et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

Insert

MTH1 (NUDT1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

May 29, 2009

Availability

Academic Institutions and Nonprofits only
pLIA-E p27 (CC#620)

Plasmid

#15191

Depositor

Connie Cepko

Article

Dyer et al J Neurosci. 2001 Jun 15. 21(12):4259-71.

Insert

p27 (Cdkn1b Mouse)

Use

Retroviral

Tags

Flag and His

Expression

Mammalian

Available Since

June 8, 2007

Availability

Academic Institutions and Nonprofits only
pTet01

Plasmid

#78283

Purpose

mRFP1 expressed behind an ATc-inducible construct

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

Tet inducible mRFP1

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pFH2.106_PB-EPN24-MCP_I-T2A-eGFP-PGK-Blast

Plasmid

#205551

Purpose

Express EPN24-MCP-I-T2A-eGFP and blasticidin resistance marker

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

EPN24-MCP-I

Use

Piggybac

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available Since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pDN34-BiNLS-hYAP1-1delta

Plasmid

#169471

Purpose

expresses optoYAP in mammalian cells

Depositor

Timothy Saunders

Article

Toh et al EMBO Rep. 2022 Sep 5;23(9):e54401. doi:

Insert

YAP1-1delta (YAP1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pOT_1 - lenti-EFS-LTBR-2A-puro

Plasmid

#181970

Purpose

Expresses human LTBR linked to puromycin resistance via P2A for lentiviral delivery.

Depositor

Neville Sanjana

Article

Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

Insert

LTBR (LTBR Human)

Use

Lentiviral

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pDAS12395_PEAR-GFP_2.0-preedited

Plasmid

#177185

Purpose

PEAR-GFP3 control plasmid with split EGFP and intact 5' splice site

Depositor

Ervin Welker

Article

Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

Insert

EGFP split with an intron between amino acids 95-96

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pDAS12125_PEAR-GFP

Plasmid

#177178

Purpose

PEAR-GFP target plasmid with split EGFP and disrupted 5' splice site

Depositor

Ervin Welker

Article

Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

Insert

EGFP split with an intron between amino acids 95-96

Use

CRISPR

Expression

Mammalian

Mutation

disrupted 5' splice site

Promoter

CMV

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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