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pLenti-CaMKIIa-Mac 3.0-EYFP

Plasmid

#35515

Purpose

Lentiviral expression of CaMKIIa-driven Mac 3.0 for optical inhibition.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Insert

eMac 3.0-EYFP

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Mutation

Trafficking signal, ER export

Promoter

CaMKIIa

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only

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pCMV5 MEKK4alpha KD

Plasmid

#12188

Depositor

Gary Johnson

Article

Gerwins et al J Biol Chem. 1997 Mar 28. 272(13):8288-9

Insert

MEKK4 (Map3k4 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Kinase dead: K1361M

Available Since

Sept. 22, 2006

Availability

Academic Institutions and Nonprofits only
pRSET a MatryoshCaMP6s

Plasmid

#100019

Purpose

Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.

Depositor

Wolf Frommer

Article

Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

Insert

MatryoshCaMP6s

Tags

6x HIS tag and Xpress_EK tag

Expression

Bacterial

Promoter

T7

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX SH2-B-SH2

Plasmid

#46479

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

SH2-B (SH2B1 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 515-639

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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MSP2253

Plasmid

#70704

Purpose

Bacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #1

Depositor

Keith Joung

Article

Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

Inserts

mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
SaCas9 sgRNA (+84)

Use

CRISPR

Tags

NLS-3xFLAG

Expression

Bacterial

Mutation

E782K, N968K and R1015H in SaCas9

Promoter

T7

Available Since

Dec. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX ShcD-SH2

Plasmid

#46488

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

ShcD (SHC4 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 512-623

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pME-CreLite

Plasmid

#131782

Purpose

Middle entry vector (Tol2 kit) with bi-cistronic CreLite system components, PhyBΔCreC and PIF6CreN, linked by P2A.

Depositor

George Eisenhoffer

Article

Yen et al Dev Dyn. 2020 Nov;249(11):1394-1403. doi

Insert

PhyBCreC-P2A-PIF6CreN

Use

Cre/Lox and Gateway Cloning

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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GEV 1

Plasmid

#12615

Depositor

John Louis

Article

Huth et al Protein Sci. 1997 Nov . 6(11):2359-64.

Insert

GB1 domain of protein G as N terminal tag, with Bcl2 as insert (Bcl2 Mouse)

Tags

G protein and His

Expression

Bacterial

Available Since

Sept. 22, 2006

Availability

Academic Institutions and Nonprofits only

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pCellulose

Plasmid

#36300

Depositor

Jay Keasling

Article

Bokinsky et al Proc Natl Acad Sci U S A. 2011 Dec 13;10

Insert

pCellulose

Use

Synthetic Biology

Promoter

Two promoters from the E coli genome drive two di…

Available Since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only

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ZIKV_Trigger_27B

Plasmid

#75008

Purpose

Portion of Zika Virus genome (KU312312: 1348-1494) containing sensor 27B trigger sequence

Depositor

James Collins, Alexander Green

Article

Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

Insert

ZIKV_Trigger_27B

Expression

Bacterial

Promoter

T7

Available Since

Feb. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pMS1: pHelper(AvCAST)_AvPSP1

Plasmid

#168133

Purpose

Inducible expression of AvCAST proteins with crRNA targeting AvPSP1.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

AvCAST minimal CRISPR array (with spacer for AvPSP1)
AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
AvTnsD

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pHBS952 TARDBP-Exon1-ko-sgRNA1-SpCas9

Plasmid

#107857

Purpose

To knock-out TDP43

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

gRNA against TARDBP (Exon 1) (TARDBP Human)

Expression

Mammalian

Available Since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a(+) 6xHis-TEV-IFIT3

Plasmid

#53559

Purpose

bacterial expression of IFIT3

Depositor

Kathleen Collins

Article

Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

Insert

IFIT3 (IFIT3 Human)

Tags

6xHIS and TEV

Expression

Bacterial

Promoter

T7

Available Since

May 29, 2014

Availability

Academic Institutions and Nonprofits only
3xFRB-myc

Plasmid

#20226

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

3xFRB (MTOR Human)

Tags

myc

Expression

Mammalian

Mutation

1 FRB copy has a T2098L point mutation

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
pGEX Tec-SH2

Plasmid

#46522

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Tec (TEC Human)

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 236-351

Promoter

Tac

Available Since

Oct. 9, 2013

Availability

Academic Institutions and Nonprofits only

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pMS18: pHelper(PmcCAST)_PmcPSP1

Plasmid

#168151

Purpose

Inducible expression of PmcCAST proteins with crRNA targeting PmcPSP1.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

PmcCAST minimal CRISPR array (with spacer for PmcPSP1)
PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
PmcCAST Tns proteins (TnsAB, TnsC and TnsD)
PmcTniQ

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pRSF-G1(6CNW)RS

Plasmid

#222873

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of 6-Cyano-L-Tryptophan (6-CN-Trp) into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Article

Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024

Insert

tRNA synthetase

Expression

Bacterial

Promoter

T7

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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3xFKBP-myc

Plasmid

#20213

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

3xFKBP (FKBP1A Human)

Tags

myc

Expression

Mammalian

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
Vh-HA-3xFRB

Plasmid

#20223

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

3xFRB (MTOR Human)

Tags

HA and Vh

Expression

Mammalian

Mutation

One copy of FRB has a T2098L mutation

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
Cry2PHR.mCh.CofS3E

Plasmid

#133619

Purpose

Expresses Cry2PHR - mCherry - CofilinS3E protein fusion

Depositor

Robert Hughes

Article

Salem et al J Biol Chem. 2020 Aug 7;295(32):11231-11

Insert

Cry2PHR - mCherry - CofilinS3E

Expression

Mammalian

Promoter

cmv

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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