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We narrowed to 3,222 results for: GAI

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Showing: 61 - 90 of 3222 results

  4. pALOD4

    Plasmid
    #111026
    Purpose
    Expresses domain 4 of anthrolysin O cholesterol sensor, has N-terminal His6-tag and two point mutations, S404C and C472A
    Depositor
    Arun Radhakrishnan
    Article
    Gay et al Biophys J. 2015 Mar 24;108(6):1459-69. d
    Insert
    Domain 4 of Anthrolysin O
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    Mutations: S404C, C472A
    Promoter
    T7
    Available Since
    June 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    111026-plasmid-map-sequence-id-214044

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  6. pW210-pPB-GA-inducible-CRISPRa-sa_dCas9-VPR-hygR

    Plasmid
    #170808
    Purpose
    PiggyBac vector to express GA-inducible VPR-Sa_dCas9 with hygromycin resistance
    Depositor
    Kenneth Yamada
    Article
    Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.
    Inserts
    GID1-VPR-IRES-HygR
    GAI-tagBFP-Sa_dCas9 (GAI Synthetic, Mustard Weed, S. aureus)
    Expression
    Mammalian
    Mutation
    Synonymous mutation in PYL1 to remove XhoI site (…
    Available Since
    June 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    170808-plasmid-map-sequence-id-334406

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  15. SpIMPDH

    Plasmid
    #206980
    Purpose
    Streptococcus pyogenes Inosine 5′-Monophosphate Dehydrogenase (SpIMPDH, EC. 1.1.1.205). Contains His6 tag with TEV protease cleavage site in pET-28b(+) plasmid for protein expression.
    Depositor
    Vern Schramm
    Article
    Glockzin et al Biochemistry. 2022 Oct 4;61(19):2088-210
    Insert
    SpIMPDH (guaB Streptococcus pyogenes)
    Tags
    His tag (with TEV cleavage site)
    Expression
    Bacterial
    Available Since
    March 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    206980-plasmid-map-sequence-id-410397

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  17. FcD265A-FLAG

    Plasmid
    #65420
    Purpose
    Mammalian expression plasmid for murine Fc-FLAG, used for monomeric Fc-IL2 production
    Depositor
    Dane Wittrup
    Article
    Zhu et al Cancer Cell. 2015 Apr 13;27(4):489-501.
    Insert
    FcD265A-FLAG
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    D265A
    Promoter
    Modified CMV Promoter
    Available Since
    June 9, 2015
    Availability
    Academic Institutions and Nonprofits only

  23. AAV-CAG-K-SPOTIT(R271F)

    Plasmid
    #191492
    Purpose
    Green fluorescent-based opioid sensor for the kappa opioid receptor with reduced Gai coupling in AAV viral vector under a CAG promoter
    Depositor
    Wenjing Wang
    Insert
    K-SPOTIT(R271F)
    Use
    AAV
    Mutation
    R271F in OR
    Promoter
    CAG
    Available Since
    April 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    191492-plasmid-map-sequence-id-377952

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  24. AAV-CAG-M-SPOTIT2(R279F)

    Plasmid
    #191493
    Purpose
    Green fluorescent-based opioid sensor for the mu opioid receptor with reduced Gai coupling in AAV viral vector under a CAG promoter
    Depositor
    Wenjing Wang
    Insert
    M-SPOTIT2(R279F)
    Use
    AAV
    Mutation
    R279F in OR
    Promoter
    CAG
    Available Since
    April 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    191493-plasmid-map-sequence-id-377870

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  25. AAV-CAG-K-red-SPOTIT(R271F)

    Plasmid
    #191494
    Purpose
    Red fluorescent-based opioid sensor for the kappa opioid receptor with reduced Gai coupling in AAV viral vector under a CAG promoter
    Depositor
    Wenjing Wang
    Insert
    K-red-SPOTIT(R271F)
    Use
    AAV
    Mutation
    R271F in OR
    Promoter
    CAG
    Available Since
    April 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    191494-plasmid-map-sequence-id-377895

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  26. AAV-CAG-M-red-SPOTIT2(R279F)

    Plasmid
    #191495
    Purpose
    Red fluorescent-based opioid sensor for the mu opioid receptor with reduced Gai coupling in AAV viral vector under a CAG promoter
    Depositor
    Wenjing Wang
    Insert
    M-red-SPOTIT2(R279F)
    Use
    AAV
    Mutation
    R279F in OR
    Promoter
    CAG
    Available Since
    April 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    191495-plasmid-map-sequence-id-377935

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Showing: 61 - 90 of 3222 results