We narrowed to 22,195 results for: his

1. pFastBacMam2_N_His8_eGFP_TEV

   Plasmid

   #178806

   Purpose

   Baculovirus gene transfer into Mammalian cells to product recombinant proteins

   Depositor

   Kazuhiro Abe

   Type

   Empty backbone

   Use

   Tags

   His8, eGFP, TEV

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. P22_wtCP_SP141his

   Plasmid

   #122651

   Purpose

   Expression of P22 virus like particle in bacteria

   Depositor

   Trevor Douglas

   Insert

   P22 Coat: WT / SP: 6xHis-SP141
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   truncated SP, P120H in the capsid scaffold (see …

   Promoter

   Available Since

   Aug. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDM_T7_HisLacZomega

   Plasmid

   #118815

   Purpose

   T7 RNAP-driven expression of N-terminal His-tagged lacZ omega subunit.

   Depositor

   Alexander Green

   Article

   Ma et al Synth Biol (Oxf). 2018;3(1):ysy018. doi:

   Insert

   lacZ_
   

   Use

   Tags

   histag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pelBHisTEV

   Plasmid

   #157741

   Purpose

   empty vector; TEV-protease-removable N-term PelB signal sequence +His10; potential C-term His6; T7lac promoter; Kan-R; avoids added residues in final protein since cloning uses BseRI restriction sites

   Depositor

   Debra Hansen

   Type

   Empty backbone

   Use

   Tags

   PelB signal sequence + His10-tag + TEV protease c…

   Expression

   Bacterial

   Mutation

   Promoter

   T7lac

   Available Since

   June 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHis17_ParM _1

   Plasmid

   #78201

   Purpose

   Expression of single-cysteine variant of plasmid segregation protein, ParM, as scaffold for ADP biosensor

   Depositor

   Martin Webb

   Article

   Kunzelmann et al J Biol Chem. 2009 Nov 27;284(48):33130-8

   Insert

   parM (K33A I27C T174A T175N C287A)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   K33A I27C T174A T175N C287A

   Promoter

   unknown

   Available Since

   July 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. hiSIIN

   Plasmid

   #185662

   Purpose

   shApc-expressing lentiviruses with varius levels of expression of SIINFEKL

   Depositor

   Tyler Jacks

   Article

   Westcott et al Nat Cancer. 2021 Oct;2(10):1071-1085. do

   Inserts

   mScarlet
   miR-30a
   shApc
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Ef1a

   Available Since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSynapsin_NLS_GFP_P2A_His6_GluA2

   Plasmid

   #170453

   Purpose

   Expresses histidine tagged GluA2 in neuronal cells

   Depositor

   Shigeki Watanabe

   Article

   Li et al Nat Commun. 2021 Jan 29;12(1):677. doi:

   Insert

   GluA2 (Gria2 Mouse)
   

   Use

   Lentiviral

   Tags

   Hexa-histidine

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available Since

   July 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. His6_MBP_FXR2P_P474S_P493N

   Plasmid

   #198695

   Purpose

   Expression of human FXR2 protein in E. coli

   Depositor

   Simpson Joseph

   Article

   Edwards et al Sci Rep. 2020 Sep 28;10(1):15858. doi: 1

   Insert

   FXR2P (FXR2 Human)
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Changed proline 474 to serine and proline 493 asp…

   Promoter

   T7

   Available Since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFastBacMam3_N_His8_eGFP_3C

   Plasmid

   #178807

   Purpose

   Baculovirus gene transfer into Mammalian cells to product recombinant proteins.

   Depositor

   Kazuhiro Abe

   Type

   Empty backbone

   Use

   Tags

   His8, eGFP, 3C

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCDF_6xHIS_Csx29

    Plasmid

    #197613

    Purpose

    For protein purification of Csx29

    Depositor

    Feng Zhang

    Article

    Strecker et al Science. 2022 Nov 25;378(6622):874-881.

    Insert

    6-HIS tagged Csx29
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28aAviHisCD72cCTLD

    Plasmid

    #129066

    Purpose

    generation of biotinylated mouse CD72c CTLD protein using bacteria

    Depositor

    Takeshi Tsubata

    Article

    Akatsu et al J Exp Med. 2016 Nov 14;213(12):2691-2706

    Insert

    CD72c (Cd72 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFastBacMam4_N_Flag_His8_eGFP_TEV

    Plasmid

    #178808

    Purpose

    Baculovirus gene transfer into Mammalian cells to product recombinant proteins.

    Depositor

    Kazuhiro Abe

    Type

    Empty backbone

    Use

    Tags

    Flag, His8, eGFP, TEV

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. HisMS2_VLP_RSV

    Plasmid

    #175958

    Purpose

    Generation of MS2 Virus-Like Particles packaged with the sequence for the RSV P and M genes [GenBank: M74568.1]

    Depositor

    Paul Freemont

    Article

    Unruh et al medRxiv 2024.04.17.24305965

    Inserts

    P gene
    M gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pHis57

    Plasmid

    #211562

    Purpose

    Amber Suppressor tRNA for Histidine

    Depositor

    Aron Fenton, Liskin Swint-Kruse

    Insert

    tRNAHis
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Synthetic (Miller 1986)

    Available Since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mAMCase_CatD_6xHis_pTwistCMV

    Plasmid

    #200228

    Purpose

    Expresses mouse Acidic Mammalian Chitinase catalytic domain with C-terminal 6xHis tag in pTwist CMV vector

    Depositor

    James S Fraser

    Article

    Diaz et al Elife. 2024 Jun 17;12:RP89918. doi: 10.7

    Insert

    Acidic Mammalian Chitinase (Chia1 Mouse)
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    S354F

    Promoter

    CMV

    Available Since

    Sept. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBADHISTEV_PfeE

    Plasmid

    #129120

    Purpose

    pBADHISTEV carrying pfeE for overexpression of PfeE in E. coli

    Depositor

    James Naismith

    Article

    Perraud et al ACS Chem Biol. 2018 Sep 21;13(9):2603-26

    Insert

    Periplasmic Esterase PfeE (PA2689 Pseudomonas aeruginosa PAO1)
    

    Use

    Tags

    TEV protease cleavable 6xHis

    Expression

    Bacterial

    Mutation

    Signal peptide sequence has been removed

    Promoter

    PBAD

    Available Since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. His6_BcLOV4_mCherry_BamUK

    Plasmid

    #114596

    Purpose

    Encodes mCherry-tagged BcLOV4 for expression in bacterial cells

    Depositor

    Brian Chow

    Article

    Glantz et al Proc Natl Acad Sci U S A. 2018 Aug 14;11

    Insert

    BcLOV4
    

    Use

    Tags

    His6 and mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. 6x-His_A1aY1_GFP

    Plasmid

    #158753

    Purpose

    Bacterial expression of 6xHis-A1aY1 fused with carboxy terminus GFP for recombinant purification

    Depositor

    Minhajuddin Sirajuddin

    Article

    Kesarwani et al J Cell Biol. 2020 Oct 5;219(10). pii: 15

    Insert

    6xHistidine_A1aY1_GFP
    

    Use

    Tags

    6x-Histidine tag, GFP, T7 leader sequence, and Th…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFastBacMam5_N_Flag_His8_eGFP_3C

    Plasmid

    #178816

    Purpose

    Baculovirus gene transfer into Mammalian cells to product recombinant proteins.

    Depositor

    Kazuhiro Abe

    Type

    Empty backbone

    Use

    Tags

    Flag, His8, eGFP, 3C

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pOPINVHH_his

    Plasmid

    #210405

    Purpose

    For expression of VHH with C-terminal his tag

    Depositor

    Ray Owens

    Article

    Eyssen et al Bio Protoc. 2024 Mar 20;14(6):e4962. doi

    Type

    Empty backbone

    Use

    Tags

    his6

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available Since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pFastBacMam6_C_3C_His8_Stop

    Plasmid

    #178810

    Purpose

    Baculovirus gene transfer into Mammalian cells to product recombinant proteins.

    Depositor

    Kazuhiro Abe

    Type

    Empty backbone

    Use

    Tags

    3C, His8, Stop codon

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGagMBPHis

    Plasmid

    #42176

    Depositor

    James Hurley

    Article

    Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

    Insert

    Gag protein sequence is from HIV-1 clone NL4-3
    

    Use

    Tags

    6xHIS and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 25, 2013

    Availability

    Academic Institutions and Nonprofits only

23. p2Bac.His.XPB

    Plasmid

    #39216

    Depositor

    Aziz Sancar

    Insert

    XPB (ERCC3 Human)
    

    Use

    Tags

    His

    Expression

    Insect

    Mutation

    Promoter

    Pph

    Available Since

    Aug. 24, 2012

    Availability

    Academic Institutions and Nonprofits only

24. p2Bac.His.XPD

    Plasmid

    #39217

    Depositor

    Aziz Sancar

    Insert

    XPD (ERCC2 Human)
    

    Use

    Tags

    His (HHYHHH)

    Expression

    Insect

    Mutation

    XPD starts at aa4

    Promoter

    Pph

    Available Since

    Aug. 24, 2012

    Availability

    Academic Institutions and Nonprofits only

25. pBIG1b_10xHisTEVATG5_ATG12

    Plasmid

    #190861

    Purpose

    Plasmid for the expression of ATG5 and ATG12 protein subunit complexes. Internal References: SMC1178

    Depositor

    Sascha Martens

    Article

    Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

    Inserts

    ATG5 (ATG5 Human)
    ATG12 (ATG12 Human)
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial and Insect

    Mutation

    Promoter

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pTAMAHISTEV_PfeAQ482A

    Plasmid

    #128945

    Purpose

    expresses Q482A mutant of PfeA in E. coli

    Depositor

    James Naismith

    Article

    Moynie et al Nat Commun. 2019 Aug 14;10(1):3673. doi:

    Insert

    ferric enterobactin receptor
    

    Use

    Tags

    TEV protease cleavable 7xHis and TamA

    Expression

    Bacterial

    Mutation

    Signal peptide sequence has been removed (1-75), …

    Promoter

    T7

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET28_Human_SAT1_Cterm_His

    Plasmid

    #220487

    Purpose

    heterologous expression of SSAT1 for protein purification

    Depositor

    Emily Balskus

    Article

    Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

    Insert

    SSAT1 (SAT1 Human)
    

    Use

    Tags

    6xHs

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET28_Human_SAT1_Nterm_His

    Plasmid

    #220482

    Purpose

    heterologous expression of SSAT1 for protein purification

    Depositor

    Emily Balskus

    Article

    Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

    Insert

    SSAT1 (SAT1 Human)
    

    Use

    Tags

    6xHs

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET28a_speG_Cterm_His

    Plasmid

    #220483

    Purpose

    heterologous expression for protein purification

    Depositor

    Emily Balskus

    Article

    Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

    Insert

    speG
    

    Use

    Tags

    6xHs

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET28a_PA4114_Cterm_His

    Plasmid

    #220484

    Purpose

    heterologous expression of PA4114 for protein purification

    Depositor

    Emily Balskus

    Article

    Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

    Insert

    PA4114
    

    Use

    Tags

    6xHs

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. HisMS2_VLP_FluA

    Plasmid

    #175955

    Purpose

    Generation of MS2 Virus-Like Particles packaged with the sequence for the Influenza A Matrix Protein [A/Illinois/20/2018(H1N1)]

    Depositor

    Paul Freemont

    Article

    Unruh et al medRxiv 2024.04.17.24305965

    Insert

    M gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

32. pMGAHisTev5B

    Plasmid

    #37221

    Depositor

    Jon Lorsch

    Article

    Acker et al Methods Enzymol. 2007;430:111-45.

    Insert

    eIF5B
    

    Use

    Tags

    His

    Expression

    Mutation

    N-term truncation (contains aa 396-1002)

    Promoter

    Available Since

    Aug. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

33. pHisIHFαIHFβ

    Plasmid

    #149384

    Purpose

    Expression of P. aeruginosa PA14, 6xHis-HRV3C-IHFalpha and IHFbeta

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Inserts

    Pseudomonas aeruginosa PA14 6xHis-tagged IHFalpha
    Pseudomonas aeruginosa PA14 IHFbeta
    

    Use

    Tags

    6xHis tag and HRV3C cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pHisAlix

    Plasmid

    #42576

    Depositor

    James Hurley

    Article

    Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

    Insert

    ALIX (PDCD6IP Human)
    

    Use

    Tags

    HIS-TEV

    Expression

    Bacterial

    Mutation

    silent mutation A1617G (numbering in ALIX gene)

    Promoter

    Available Since

    Dec. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

35. pOPINVHH_cys_his

    Plasmid

    #210403

    Purpose

    For expression of VHH with C-terminal cysteine and his tag

    Depositor

    Ray Owens

    Article

    Eyssen et al Bio Protoc. 2024 Mar 20;14(6):e4962. doi

    Type

    Empty backbone

    Use

    Tags

    cys-his6

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available Since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pOPINVHH_sortase_his

    Plasmid

    #210406

    Purpose

    For expression of VHH with C-terminal sortase and his tags

    Depositor

    Ray Owens

    Article

    Eyssen et al Bio Protoc. 2024 Mar 20;14(6):e4962. doi

    Type

    Empty backbone

    Use

    Tags

    sortase-his6

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available Since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pOPINVHH_snap_his

    Plasmid

    #210407

    Purpose

    For expression of VHH with C-terminal snap protein and his tag

    Depositor

    Ray Owens

    Article

    Eyssen et al Bio Protoc. 2024 Mar 20;14(6):e4962. doi

    Type

    Empty backbone

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available Since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pOPINVHH_his_myc

    Plasmid

    #210526

    Purpose

    For expression of VHH with C-terminal his and myc tags

    Depositor

    Ray Owens

    Article

    Eyssen et al Bio Protoc. 2024 Mar 20;14(6):e4962. doi

    Type

    Empty backbone

    Use

    Tags

    His6-myc

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available Since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. BS-His3

    Plasmid

    #69197

    Purpose

    complementation of yeast auxotrophic marker HIS3

    Depositor

    Zhiping Xie

    Article

    Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

    Insert

    HIS3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pDEST8_FLAGHA_FUS_HIS

    Plasmid

    #26375

    Depositor

    Thomas Tuschl

    Article

    Hoell et al Nat Struct Mol Biol. 2011 Nov 13. doi: 1

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    FLAG/HA and His

    Expression

    Insect

    Mutation

    delta 527 (STOP codon)

    Promoter

    Available Since

    Oct. 18, 2010

    Availability

    Academic Institutions and Nonprofits only