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Showing: 41 - 60 of 276 results

  1. pRS416-Gal4-dCas9-VP64

    Plasmid
    #71128
    Purpose
    This plasmid contains a Cas9 Activator for yeast. The activator is about 1.2-1.8 X more potent than just dCas9-VP64 fusion in yeast. It is on a single copy CEN/ARS plasmid with Ura marker, pRS416.
    Depositor
    Ronald Davis
    Article
    Naranjo et al PLoS Genet. 2015 Dec 29;11(12):e1005751.
    Insert
    Gal4-dCas9-VP64
    Use
    Tags
    NLS n-terminal, N terminal Gal4 Activator domain,…
    Expression
    Yeast
    Mutation
    Promoter
    Tef1
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    71128-plasmid-map-sequence-id-127063

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  2. pLV hU6-gRNA(anti-sense) hUbC-VP64-dCas9-VP64-T2A-GFP

    Plasmid
    #66707
    Purpose
    expresses a single gRNA and VP64-dCas9-VP64
    Depositor
    Charles Gersbach
    Inserts
    hU6-gRNA expression cassette
    S.Pyogenes VP64-dCas9-VP64
    Use
    Lentiviral and Synthetic Biology
    Tags
    VP64
    Expression
    Mutation
    D10A and H840A
    Promoter
    hU6 and hUbC
    Available since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    66707-plasmid-map-sequence-id-244291

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  4. lenti-UCOE-dCas9-BFP-VP64

    Plasmid
    #196717
    Purpose
    CRISPR-activation. dCas9-VP64 with TagBFP for sorting.
    Depositor
    Bernhard Schmierer
    Insert
    dCas9-TagBFP-VP64
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Expression
    Mutation
    D10A, N863A
    Promoter
    EF1a
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196717-plasmid-map-sequence-id-388038

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  6. pMSCV-LTR-dCas9-VP64-BFP

    Plasmid
    #46912
    Purpose
    Human expression vector containing MSCV LTR promoter, dCas9 that is fused to 2x NLS, VP64 and tagBFP
    Depositor
    Jonathan Weissman, Stanley Qi
    Article
    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008
    Inserts
    dCas9-VP64-BFP fusion
    Puromycin resistance
    Use
    CRISPR and Retroviral
    Tags
    3xNLS, BFP, and VP64 domain
    Expression
    Mammalian
    Mutation
    Promoter
    LTR and PGK
    Available since
    Oct. 1, 2013
    Availability
    Academic Institutions and Nonprofits only
    46912-plasmid-map-sequence-id-66610

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  19. pPB-Ins-TRE3Gp-dCas9-Suntag-VP64-EF1Ap-Puro

    Plasmid
    #183409
    Purpose
    piggyBAC-based doxycycline-inducible dCas9-5xSuntag-VP64 CRISPR activation plasmid for enhancer and promoter activation in mammalian cells
    Depositor
    Azim Surani
    Article
    Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d
    Insert
    dCas9-5xGCN5-P2A-scFV-sfGFP-VP64-GB1
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G
    Available since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    183409-plasmid-map-sequence-id-361258

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Showing: 41 - 60 of 276 results