We narrowed to 883 results for: golden gate assembly
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Plasmid#223884Purpose4G-cloning donor for N-terminal His10-TwinStrep-3C tagDepositorInsertHis10-TwinStrep-3C
UseGolden-gate donorAvailable SinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Ct)_3C-Venus-His8
Plasmid#223889Purpose4G-cloning donor for C-terminal 3C-Venus-His8 tagDepositorInsert3C-Venus-His8
UseGolden-gate donorAvailable SinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(P+T)_CMVC
Plasmid#223862Purpose4G-cloning donor for mammalian promoter and terminator, C-terminal tag addedDepositorInsertCMV promoter and bGH polyA
UseGolden-gate donorAvailable SinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUCC001
Plasmid#124451PurposeMulti-species CRISPR/Cas9 coexpression system, optimized for Golden Gate cloning of gRNA targetsDepositorInsertHH-BsaI
ExpressionYeastMutationgRNA expression cassette from pUDP002 modified to…PromoterTDH3 promoter from S.cerevisiae (already present …Available SinceSept. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGTRm
Plasmid#127197PurposepGTRm is the template plasmid for PCR & golden gate assembly driven generation of polycistronic tRNA-gRNA sequencesDepositorInserttRNA-gRNA PCR template
UseTemplate plasmid for generation of golden gate pc…PromoterNoneAvailable SinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBT100-neo
Plasmid#123137PurposeGolden Gate donor vector for the assembly of EGFP-based mammalian recombinase reporters.DepositorInsertNeomycin, poly-A terminator
UseSynthetic Biology; Golden gate donor vectorPromoternoneAvailable SinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
HSA_3b*_AP
Plasmid#238899PurposePlasmid insert contains a gene sequence for Human serum albumin for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).DepositorInsertRecombinant Human Serum Albumin (HSA) 3b*
UseSynthetic BiologyAvailable SinceSept. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
C-term_TEV_aMFx1 (4a)
Plasmid#238903PurposePlasmid insert contains the aMFx1 C-terminal tag sequence with the TEV cleavage site for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).DepositorInsertC-term_TEV_aMFx1
UseSynthetic BiologyAvailable SinceSept. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
C-term_C-myc (4a)
Plasmid#238907PurposePlasmid insert contains the C-myc C-terminal tag sequence for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).DepositorInsertC-term_C-myc
UseSynthetic BiologyAvailable SinceSept. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
SP_H3
Plasmid#228189PurposePlasmid contains the secretion signal peptide sequence of the yeast CIS3 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).DepositorInsertsp_P47001_CIS3_YEAST:0-21
UseSynthetic BiologyAvailable SinceJuly 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
SP_H4
Plasmid#228190PurposePlasmid contains the secretion signal peptide sequence of the human CCL8 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).DepositorInsertsp_P80075_CCL8_HUMAN:0-23
UseSynthetic BiologyAvailable SinceJuly 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
SP_H5
Plasmid#228191PurposePlasmid contains the secretion signal peptide sequence of the human SDC4 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).DepositorInsertsp_P31431_SDC4_HUMAN:0-20
UseSynthetic BiologyAvailable SinceJuly 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
SP_H6
Plasmid#228192PurposePlasmid contains the secretion signal peptide sequence of the human BPIB4 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).DepositorInsertsp_P59827_BPIB4_HUMAN:0-17
UseSynthetic BiologyAvailable SinceJuly 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
SP_F2
Plasmid#228176PurposePlasmid contains the secretion signal peptide sequence of the yeast EXG1 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).DepositorInsertsp_P23776_EXG1_YEAST:0-19
UseSynthetic BiologyAvailable SinceJuly 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
SP_F3
Plasmid#228177PurposePlasmid contains the secretion signal peptide sequence of the yeast ECM33 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).DepositorInsertsp_B5VE42_ECM33_YEAS6:0-19
UseSynthetic BiologyAvailable SinceJuly 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
SP_F4
Plasmid#228178PurposePlasmid contains the secretion signal peptide sequence of the human CCL2 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).DepositorInsertsp_P13500_CCL2_HUMAN:0-23
UseSynthetic BiologyAvailable SinceJuly 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
SP_F5
Plasmid#228179PurposePlasmid contains the secretion signal peptide sequence of the human MMP11 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).DepositorInsertsp_P24347_MMP11_HUMAN:0-33
UseSynthetic BiologyAvailable SinceJuly 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
SP_F6
Plasmid#228180PurposePlasmid contains the secretion signal peptide sequence of the human PDGFC gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).DepositorInsertsp_Q9NRA1_PDGFC_HUMAN:0-22
UseSynthetic BiologyAvailable SinceJuly 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
SP_G1
Plasmid#228181PurposePlasmid contains the secretion signal peptide sequence of the yeast DSE2 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).DepositorInsertsp_P38844_DSE2_YEAST:0-21
UseSynthetic BiologyAvailable SinceJuly 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
SP_G2
Plasmid#228182PurposePlasmid contains the secretion signal peptide sequence of the yeast PRY1 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).DepositorInsertsp_P47032_PRY1_YEAST:0-19
UseSynthetic BiologyAvailable SinceJuly 2, 2025AvailabilityAcademic Institutions and Nonprofits only