We narrowed to 16,014 results for: grna
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Plasmid#198728PurposeExpresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Neither gRNA is fused to a mobility signal and are therefore non-mobile.DepositorInsertAtNIA1 gRNAs
ExpressionPlantPromoterpU6-26, pU6-29Available sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pU6-sp-sgRNA-RNF2_+41nick
Plasmid#135958PurposeS. pyogenes sgRNA for +41 nick in RNF2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRNF2_+41 nicking sgRNA
ExpressionMammalianMutationSee manuscriptPromoterU6Available sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
LentiGuide_DKO
Plasmid#183193PurposePlasmid with two U6 promoters and two gRNA scaffolds that allows for inserting two gRNAs for combinatorial CRISPR Screen or double-knock-out (DKO) screenDepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianPromoterhU6, mU6Available sinceAug. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSOX2.1.0-gDNA
Plasmid#176382PurposeExpresses spCas9 and gRNA sequence targeting SOX2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSOX2 gRNA
UseCRISPRAvailable sinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-U6-EMX1
Plasmid#140581PurposeExpresses a gRNA and mCherry in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertgRNA EMX1
UseCRISPRAvailable sinceMay 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
PX458_GABPA_1
Plasmid#64254PurposeExpresses gRNA against human GABPA along with Cas9 with 2A GFPDepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertgRNA
UseCRISPRAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
eSpCas9(1.1)_No_FLAG_ATP1A1_G2_Dual_sgRNA
Plasmid#86612PurposeVector for tandem expression of ATP1A1 G2 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmidDepositorInsertATP1A1 G2 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)
UseCRISPR; Co-selection via nhej using ouabainExpressionMammalianMutationK848A, K1003A, & R1060APromoterCBhAvailable sinceMarch 16, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEasyG1_zeo
Plasmid#184910PurposeTemplate to generate via PCR a single gRNA for expression in S. cerevisiaeDepositorInsertgRNA scaffold
UseCRISPRExpressionBacterialAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pORANGE Rims1-GFP KI
Plasmid#131494PurposeEndogenous tagging of RIM1: C-terminal (amino acid position: S1615)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertgRNA and GFP donor (Rims1 Rat)
ExpressionMammalianPromoterU6 and CbhAvailable sinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCfB8792
Plasmid#126910PurposeTemplate for PCR amplification of ADE2 gRNA biobrick for targeting Saccharomyces cerevisiae ADE2 geneDepositorInsertADE2 gRNA
UseSynthetic BiologyAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
U6a-T
Plasmid#117209PurposeExpresses gRNA in Aedes aegyptiDepositorInsertu6a promoter-gRNA
UseSynthetic BiologyExpressionInsectPromoterU6Available sinceJan. 23, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV enAsCas12f-HKRA-sgRNA_v7
Plasmid#208357PurposeExpression of enAsCas12f-HKRA and sgRNA_v7 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertenAsCas12f-HKRA
ExpressionMammalianMutationI123H/D195K/D208R/V232APromoterCMVAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(gGFP)-PGKmCherry2AGFP-W
Plasmid#67982PurposeCas9 activity reporter with mCherry and GFPDepositorInsertU6gRNA cassette, PGKmCherry2AGFP cassette, WPRE
UseCRISPR and LentiviralMutationDeleted BbsI site within WPREAvailable sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pROS12
Plasmid#107926Purposehph based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacerDepositorInsertgRNA-CAN1.Y, gRNA-ADE2.Y
UseCRISPRExpressionYeastAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRPR1_c4gRNA_RPR1t
Plasmid#64382Purposeencodes c4 gRNADepositorPublicationInsertc4 gRNA
ExpressionYeastAvailable sinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRPR1_c8gRNA_RPR1t
Plasmid#64386Purposeencodes c8 gRNADepositorPublicationInsertc8 gRNA
ExpressionYeastAvailable sinceMay 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJR104
Plasmid#187243PurposeLentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-GFP marker expressionDepositorInsertLentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-GFP marker expression
UseLentiviralAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEasyG3_hph
Plasmid#184919PurposeTemplate to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_hph recombines in vivo with a PCR product from pEasyG3_mic.DepositorInsertgRNA scaffold
UseCRISPRExpressionBacterialAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-SFFV-Zim3-dCas9-P2A-Hygro
Plasmid#188768PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9
UseLentiviralTagsHA-2xNLS and Zim3 KRAB-NLS fusionPromoterSFFVAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJEP316-pAAV-U6SaCas9gRNA(SapI)-pA Empty Cassette
Plasmid#113693PurposeU6 driven SaCas9 gRNA expression cassette without a gRNA. SapI can be used to clone in gRNAs.DepositorInsertSaCas9 gRNA Cassete
UseAAV and CRISPRAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only