We narrowed to 1,256 results for: grna cloning vector

1. pU6-Mb-crRNA

   Plasmid

   #78959

   Purpose

   Cloning vector for expression of MbCpf1 crRNA. It contains BsmB1 site for cloning.

   Depositor

   Jin-Soo Kim

   Article

   Kim et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available since

   June 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPV-H1-ccdB-mEF1α-RiH

   Plasmid

   #100598

   Purpose

   piggyBac vector for sgRNA cloning

   Depositor

   Akitsu Hotta

   Article

   Ishida et al Sci Rep. 2018 Jan 10;8(1):310. doi: 10.1

   Type

   Empty backbone

   Use

   Piggybac vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human H1 PolIII promoter, human FTH1 promoter, mo…

   Available since

   April 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. hCtRNA_VT

   Plasmid

   #186716

   Purpose

   Vector template for DAP array

   Depositor

   Xue Gao

   Article

   Yuan et al Nat Commun. 2022 May 19;13(1):2771. doi:

   Insert

   hCtRNA and gRNA scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hCtRNA

   Available since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pV1081

   Plasmid

   #111425

   Purpose

   Solo vector for expression of CaCas9 and sgRNA for CaADE2 mutagenesis - integrates at ENO1

   Depositor

   Gerald Fink

   Article

   Vyas et al Sci Adv. 2015;1(3):e1500248.

   Insert

   CaCas9/sgADE-NT2
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. hU6-Sa_tracr

   Plasmid

   #135973

   Purpose

   Contains tracrRNA-hU6 sequence for double sgRNA cloning

   Depositor

   Yongming Wang

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pML104

   Plasmid

   #67638

   Purpose

   Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains URA3 marker for yeast transformation

   Depositor

   John Wyrick

   Article

   Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

   Inserts

   Cas9
   single guide RNA expression cassette
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pSNR52 and pTDH3

   Available since

   Aug. 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pML107

   Plasmid

   #67639

   Purpose

   Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains LEU2 marker for yeast transformation

   Depositor

   John Wyrick

   Article

   Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

   Inserts

   Cas9
   single guide RNA expression cassette
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pSNR52 and pTDH3 (aka GAP promoter)

   Available since

   Aug. 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. AA400

   Plasmid

   #215958

   Purpose

   Fragmid fragment: (guide cassette) guide expression

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   U6_v1; DR_v1 [Rfx]; BsmBI_v1; BsmBI_v12
   

   Use

   CRISPR; Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. AA401

   Plasmid

   #215959

   Purpose

   Fragmid fragment: (guide cassette) guide expression

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   U6_v1; DR_v1 [Dj]; BsmBI_v1; BsmBI_v12
   

   Use

   CRISPR; Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Ccas9LacZ

    Plasmid

    #113083

    Purpose

    Enhanced C. elegans Cas9 vector. Ccas9LacZ contains a lacZ coding sequence, two Cas9 and two type-IIs restriction enzyme recognition sites next to the U6 promoter and gRNA sequences.

    Depositor

    Tibor Vellai

    Article

    Sturm et al Nucleic Acids Res. 2018 Jun 19. pii: 504

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPBO.ACT005

    Plasmid

    #182715

    Purpose

    Constituitvely expressed CasRx crRNA cloning vector, truncated 3' terminator region

    Depositor

    Joseph Schoeniger

    Article

    Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

    Insert

    CasRx crRNA cloning backbone
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Catalytically deactivated R295A, H300A, R849A, H8…

    Promoter

    pJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)

    Available since

    Aug. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPBO.ACT004

    Plasmid

    #182714

    Purpose

    Constituitvely expressed CasRx crRNA cloning vector, extended 3' terminator region

    Depositor

    Joseph Schoeniger

    Article

    Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

    Insert

    CasRx crRNA cloning backbone
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Catalytically deactivated R295A, H300A, R849A, H8…

    Promoter

    pJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)

    Available since

    Aug. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. plentiCRISPR-sgMDH1

    Plasmid

    #72875

    Purpose

    The lentiviral sgMDH1 vectors was generated via ligation of hybridized oligos (below) into lentiCRISPR-v1 vector linearized with BsmBI using Gibson assembly (NEB

    Depositor

    David Sabatini

    Article

    Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10

    Insert

    sgMDH1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pCD061

    Plasmid

    #113315

    Purpose

    W108 scRNA.b1

    Depositor

    Jesse Zalatan

    Article

    Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

    Insert

    W108 scRNA.b1_MS2
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCD315

    Plasmid

    #113321

    Purpose

    pBAD-W108 scRNA.b1

    Depositor

    Jesse Zalatan

    Article

    Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

    Insert

    scRNA.b1_MS2
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLKO.puro_shHuR 3UTR

    Plasmid

    #110414

    Purpose

    TRCN0000276186 (Target TTGTTAGTGTACAACTCATTT), silence human ELAVL1 (HuR) gene, puromycin selection

    Depositor

    Sandra Martha Gomes Dias

    Insert

    ELAVL1 (HuR)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 (RNA PolIII)

    Available since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJF121

    Plasmid

    #113325

    Purpose

    pTet-W108 scRNA.b2

    Depositor

    Jesse Zalatan

    Article

    Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

    Insert

    W108 scRNA_MS2
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCD005

    Plasmid

    #113314

    Purpose

    W108 scRNA

    Depositor

    Jesse Zalatan

    Article

    Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

    Insert

    W108 scRNA_MS2
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AA290

    Plasmid

    #215949

    Purpose

    Fragmid fragment: (guide cassette) guide expression; positive control cell surface

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    U6_v1; sgCD55_v4; trRNA_v4 [Sp]
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AA286

    Plasmid

    #215948

    Purpose

    Fragmid fragment: (guide cassette) guide expression; positive control cell surface

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    U6_v1; sgCD47_v6; trRNA_v4 [Sp]
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge