We narrowed to 17,700 results for: SHE

1. pMBP-LbCas12a H759A

   Plasmid

   #113518

   Purpose

   Expresses His-MBP fused to LbCas12a with H759A mutation to inactivate crRNA processing

   Depositor

   Jennifer Doudna

   Article

   Chen et al Science. 2018 Apr 27;360(6387):436-439.

   Insert

   LbCas12a
   

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   H759A

   Available Since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOTTC533 - pAAV SYN1 eGFP-2A-mKv1.2

   Plasmid

   #75295

   Purpose

   An AAV packaging vector that expresses eGFP and Kv1.2 under the control of a neuronal promoter.

   Depositor

   Brandon Harvey

   Insert

   eGFP-2A-mKv1.2 (Kcna2 Mouse, Synthetic)
   

   Use

   AAV

   Promoter

   human SYN1

   Available Since

   Jan. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLJM1-FLAG-Sestrin2-L261A

   Plasmid

   #73676

   Purpose

   overexpression

   Depositor

   David Sabatini

   Article

   Wolfson et al Science. 2016 Jan 1;351(6268):43-8. doi:

   Insert

   Sesn2 (SESN2 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   L261A changed Leucine 261 to Alanine

   Promoter

   CMV

   Available Since

   March 30, 2016

   Availability

   Academic Institutions and Nonprofits only

4. Lamin A (R527P)-mRFP

   Plasmid

   #124272

   Purpose

   Lamin A mutant (R527P), C-terminally tagged with mRFP. Mutation associated with EDMD myopathy.

   Depositor

   Eric Schirmer

   Insert

   LMNA (R527P) (LMNA Human)
   

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   LMNA (R527P)

   Available Since

   May 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. GWF196

   Plasmid

   #133613

   Purpose

   NS3aH1-VPR

   Depositor

   Dustin Maly

   Article

   Foight et al Nat Biotechnol. 2019 Sep 9. pii: 10.1038

   Insert

   NS3aH1-VPR
   

   Expression

   Mammalian

   Available Since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. HSP90B1

   Plasmid

   #39076

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   HSP90B1 (HSP90B1 Human)
   

   Tags

   TEV-His6-Flag

   Expression

   Bacterial

   Available Since

   May 7, 2013

   Availability

   Academic Institutions and Nonprofits only

7. p-dCas9-DNMT3a-Hygro

   Plasmid

   #104403

   Purpose

   transient expression of dCas9-DNMT3a fusion protein

   Depositor

   Stephan Beck

   Insert

   DNMT3a (DNMT3A Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV promoter

   Available Since

   April 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lamin A (R482Q)-mRFP

   Plasmid

   #124273

   Purpose

   Lamin A mutant (R482Q), C-terminally tagged with mRFP. Mutation associated with FPLD lipodystrophy.

   Depositor

   Eric Schirmer

   Insert

   LMNA (R482Q) (LMNA Human)
   

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   LMNA (R482Q)

   Available Since

   May 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. SyGCaMP5G

   Plasmid

   #162519

   Purpose

   Synaptically targeted GCaMP5G

   Depositor

   Josef Kittler

   Article

   Al Awabdh et al Glia. 2016 Jul;64(7):1252-64. doi: 10.10

   Insert

   GCaMP5G
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAIO-LbCpf1

    Plasmid

    #137847

    Purpose

    LbCpf1 and gRNA expression plasmid for P. falciparum; no selection in parasites.

    Depositor

    Josh Beck

    Article

    Nessel et al Cell Microbiol. 2020 Jan 28:e13168. doi:

    Insert

    LbCpf1-NLS-FLAG
    

    Use

    CRISPR

    Available Since

    April 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. BRAF - A12G in pENTR

    Plasmid

    #216606

    Purpose

    gateway cloning donor vector containing BRAF ASD mutation

    Depositor

    Douglas Allan, Christopher Loewen

    Insert

    BRAF (BRAF Human)
    

    Use

    Gateway entry plasmid

    Mutation

    A12G

    Available Since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUASp YFP Rac1 DN

    Plasmid

    #52234

    Purpose

    Drosophila expression of YFP-Rac1 dominant negative mutant

    Depositor

    Matthew Scott

    Insert

    Rac1 (Rac1 Fly)
    

    Use

    Drosophila

    Tags

    YFP

    Expression

    Insect

    Mutation

    T17N; DN mutation

    Promoter

    GAL4

    Available Since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pET28-H12TEV-hEIF5B-1~1220(FL)

    Plasmid

    #208636

    Purpose

    for E. coli expression of human eIF5B (ENST00000289371)

    Depositor

    Quanfu Ma

    Insert

    human eIF5B (ENST00000289371) (EIF5B Human)
    

    Tags

    6xhis

    Expression

    Bacterial

    Mutation

    Contain SNP rs1376443, protein sequence not chang…

    Promoter

    T7

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1(-) Full length Her2(ERBB2)-Fc

    Plasmid

    #194330

    Purpose

    Mammalian expression of full length Human epidermal growth factor (Her)-2-Fc fusion protein

    Depositor

    Alexander McLellan

    Insert

    Her2-Fc (ERBB2 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pACP-ADRβ2 Control Plasmid

    Plasmid

    #101128

    Purpose

    Expression of ACP-Tagged β2 Adrenergic Receptor in Mammalian cells

    Depositor

    New England Biolabs, Ana Egana

    Insert

    Beta-2-Adrenergic Receptor (ADRB2 Human)
    

    Tags

    ACP-tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 27, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pS2-FLAG-Sestrin2-L261A

    Plasmid

    #73678

    Purpose

    overexpression

    Depositor

    David Sabatini

    Article

    Wolfson et al Science. 2016 Jan 1;351(6268):43-8. doi:

    Insert

    Sesn2 (SESN2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    L261A changed Leucine 261 to Alanine

    Promoter

    Sesn2

    Available Since

    April 25, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pAAV hSyn1 bPAC cMyc S27A 2A tDimer

    Plasmid

    #85398

    Purpose

    humanized photoactivated adenylyl cyclase lower dark activity, spectrum shifted 15 nm longer wavelenght with neuron-specific promoter, plus red fluorescent protein

    Depositor

    Thomas Oertner

    Inserts

    humanized photoactivated adenylyl cyclase
    red fluorescent protein
    

    Use

    AAV

    Tags

    cMyc Tag

    Expression

    Mammalian

    Mutation

    S27A

    Promoter

    human Synapsin1 promotor and ribosomal skip seque…

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. MSCV-mouse Mdm2 wild type-IRES-GFP

    Plasmid

    #180487

    Purpose

    Role of Mdm2 variants in tumorigenesis

    Depositor

    Martine Roussel

    Article

    Dang et al Cancer Res. 2002 Feb 15;62(4):1222-30.

    Insert

    Mouse

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    MSCV

    Available Since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA-NBid-mMaple-CBid

    Plasmid

    #164049

    Purpose

    Optogenetic manipulation of cell apoptosis negative control

    Depositor

    Robert Campbell

    Article

    Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

    Insert

    NBid-mMaple-CBid
    

    Tags

    kozak sequence

    Expression

    Mammalian

    Promoter

    CMV promotor

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. HSD17B11

    Plasmid

    #39159

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    HSD17B11 (HSD17B11 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    codon-optimized

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

21. Hs.KRAS4a G13D

    Plasmid

    #83170

    Purpose

    Gateway ORF Entry clone of human KRAS4A [NM_033360.3] with stop codon (for native or N-terminal fusions), G13D mutation

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Mutation

    G13D

    Available Since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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22. pDM_noro_A2_lacZ

    Plasmid

    #118820

    Purpose

    T7 RNAP-driven expression of norovirus antisense orientation toehold switches (A2) with a lacZ reporter.

    Depositor

    Alexander Green

    Article

    Ma et al Synth Biol (Oxf). 2018;3(1):ysy018. doi:

    Insert

    Norovirus toehold switch A2
    

    Expression

    Bacterial

    Available Since

    Nov. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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23. RCAS (A) Six1 (CT#664)

    Plasmid

    #13876

    Depositor

    Cliff Tabin

    Article

    Heanue et al Genes Dev. 1999 Dec 15. 13(24):3231-43.

    Insert

    Six1 (Six1 Mouse)
    

    Use

    Retroviral; Avian expression

    Available Since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

24. GWF148

    Plasmid

    #133617

    Purpose

    GNCR1-BFP-CAAX

    Depositor

    Dustin Maly

    Article

    Foight et al Nat Biotechnol. 2019 Sep 9. pii: 10.1038

    Insert

    GNCR1-BFP-CAAX
    

    Expression

    Mammalian

    Available Since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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25. Lamin A (R298C)-mRFP

    Plasmid

    #124269

    Purpose

    Lamin A mutant (R298C), C-terminally tagged with Mrfp. Mutation associated with CMT neuropathy.

    Depositor

    Eric Schirmer

    Insert

    LMNA (R298C) (LMNA Human)
    

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    LMNA (R298C)

    Available Since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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26. pBAV-B

    Plasmid

    #65928

    Purpose

    Theophylline Riboswitch B with lacZ gene

    Depositor

    Justin Gallivan

    Article

    Topp et al Appl Environ Microbiol. 2010 Dec;76(23):

    Insert

    lacZ
    

    Expression

    Bacterial

    Available Since

    Sept. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

27. pSBtet-Bla/PAG1-TurboID-GAI

    Plasmid

    #198217

    Purpose

    Transposon vector (Addgene #60510) for doxycycline-inducible expression of a trimer with human PAG1, part of TurboID (after G257), and GAI (AAs 11-93) for gibberellic acid-inducible biotin labeling

    Depositor

    Eric Davis

    Insert

    PAG1 (PAG1 Human)
    

    Use

    Transposon

    Tags

    3’ part of TurboID, starting with G257 and amino …

    Expression

    Mammalian

    Mutation

    None

    Available Since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pT2K-CAGGS-U6-stuffer-dCAs9-VP160-T2A-IRES-mCherry

    Plasmid

    #114726

    Purpose

    dCas9-VP160 expression in mammalian cells

    Depositor

    Martine Roussel

    Article

    Vo et al Sci Rep. 2018 Jun 7;8(1):8733. doi: 10.1

    Insert

    dCas9-VP160
    

    Expression

    Mammalian

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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29. OA-1055J

    Plasmid

    #200251

    Purpose

    pBac-U6-gRNA(Intersex)-3xp3-tdTomato

    Depositor

    Omar Akbari

    Article

    Li et al bioRxiv. 2023 Nov 15:2023.04.18.537404.

    Insert

    2 gRNAs targeting Intersex gene
    

    Expression

    Insect

    Available Since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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30. 3918-37

    Plasmid

    #176495

    Purpose

    pTRE-EGFP-T2A-mCherry-polyA

    Depositor

    Albert La Spada

    Article

    Hsu et al Nat Commun. 2018 Mar 5;9(1):942. doi: 10

    Insert

    Tetracyline Responsive Element driving EGFP-T2A-mCherry
    

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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