We narrowed to 9,135 results for: mel

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pcDNA3.1-Y1214F-VEGFR2-mCherry

Plasmid

#139609

Purpose

Expresses a mutant (Y1214F) VEGFR2-mCherry fusion protein in mammalian cells

Depositor

Michael Welsh

Article

Pietila et al Cells. 2019 Dec 15;8(12). pii: cells8121

Insert

KDR (KDR Human)

Expression

Mammalian

Promoter

CMV

Available Since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-GST FNIP2

Plasmid

#164982

Purpose

FNIP2 expressing vector for mammalian cells

Depositor

Roberto Zoncu

Article

Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

Insert

FNIP2 (FNIP2 Human)

Tags

GST

Expression

Mammalian

Promoter

CAG

Available Since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCrysβ:ECFP-LexOP:mCherry-HrasG12V

Plasmid

#89887

Purpose

Human HRasG12V fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening.

Depositor

Tatjana Sauka-Spengler

Article

Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

Insert

mCherry-HRasG12V

Use

Unspecified

Tags

mCherry

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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Designed VEGF-binding scFv G6des1

Plasmid

#110212

Purpose

Yeast Surface Display of G6des1

Depositor

Sarel Fleishman

Article

Warszawski et al PLoS Comput Biol. 2019 Aug 23;15(8):e100

Insert

G6des1

Tags

cmyc tag

Expression

Yeast

Mutation

L A43P, L Y49F, L Y55F ,L Q89L, L T94V, L P95T, L…

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pGL3-DICER-Prom

Plasmid

#25851

Depositor

David E. Fisher

Article

Levy et al Cell. 2010 Jun 11. 141(6):994-1005.

Insert

Dicer promoter (DICER1 gene ID 23405, Human)

Use

Luciferase

Expression

Mammalian

Available Since

Aug. 25, 2010

Availability

Academic Institutions and Nonprofits only

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pSUPER-retro-puro-shSTIM1

Plasmid

#89816

Purpose

Knockdown human STIM1 expression

Depositor

Shengyu Yang

Article

Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

Insert

STIM1 shRNA (STIM1 Human)

Use

Retroviral

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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VP-ARF

Plasmid

#89122

Purpose

mammalian expression of p14-hARF linked to VP16 activation domain

Depositor

Elizabeth Wilson

Article

Minges et al J Biol Chem. 2015 Oct 9;290(41):25174-87

Insert

p14-hARF (CDKN2A Human)

Tags

VP16

Expression

Mammalian

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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FpG5.BFP.Reporter

Plasmid

#134967

Purpose

Lentiviral vector for enhancer reporter assay

Depositor

Beat Bornhauser

Article

Huang et al Cancer Cell. 2019 Dec 9;36(6):630-644.e9

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

mini Fgf4 promoter

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pCrysβ:ECFP-LexOP:mCherry-NRasQ61K

Plasmid

#89886

Purpose

Human NRasQ61K fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening.

Depositor

Tatjana Sauka-Spengler

Article

Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

Insert

mCherry-NRasQ61K

Use

Unspecified

Tags

mCherry

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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ABE8.17-d

Plasmid

#136299

Purpose

expresses ABE8.17-d in mammalian cells

Depositor

Nicole Gaudelli

Article

Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

Insert

ABE8.20-d

Tags

C-terminal BPNLS

Expression

Mammalian

Mutation

D10A in S. pyogenes Cas9, TadA mutations describe…

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pShuttle-EGFP-CLASP2 340-1362

Plasmid

#24372

Depositor

Torsten Wittmann

Article

Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

Insert

CLASP2 (340-1362) (CLASP2 Human)

Use

Adenoviral

Tags

EGFP

Expression

Mammalian

Mutation

CLASP2 deletion mutant that retains MT lattice bi…

Available Since

June 29, 2010

Availability

Academic Institutions and Nonprofits only

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pMCB320-sgNC.SPA

Plasmid

#169834

Purpose

Expresses a negative control sgRNA

Depositor

Laura Attardi

Article

Bieging-Rolett et al Mol Cell. 2020 Nov 5;80(3):452-469.e9. d

Insert

safe harbor sgRNA

Use

CRISPR, Lentiviral, and Mouse Targeting

Expression

Mammalian

Promoter

mU6

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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pDropIn-T2A-split-intein-Gal4[21-881]-C-int

Plasmid

#199196

Purpose

Insert to create split-intein Gal4[C-int] knock-in vectors via Drop-In cloning, released via BsmBI digest

Depositor

Norbert Perrimon

Article

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

T2A-split-intein-Gal4[21-881]-C-int, 3xP3-dsRed

Expression

Insect

Available Since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pEB3-miRFP703

Plasmid

#79994

Purpose

EB3 (microtubule-associated protein) labeling with near-infrared fluorescent protein miRFP703

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

EB3 (MAPRE3 Human)

Tags

miRFP703

Expression

Mammalian

Promoter

CMV

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shQKI-3371

Plasmid

#115455

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

shQKI-3371 (QKI Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a(ICOS)

Plasmid

#177903

Purpose

Bacterial expression of his10-tagged human ICOS Intracellular Domain

Depositor

Ron Vale

Article

Hui et al Science. 2017 Mar 31;355(6332):1428-1433

Insert

ICOS (aa 162-199 only) (ICOS Human)

Tags

10xHis-Thrombin cut site-T7 tag

Expression

Bacterial

Promoter

T7

Available Since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-Phi3-Zsgreen-PGK-mCherry

Plasmid

#153320

Purpose

Expresses zsGreen and mCherry in mammalian cells.

Depositor

Robert Judson-Torres

Article

McNeal et al Elife. 2021 Nov 23;10. pii: 70385. doi:

Insert

zsGreen

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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GAL-FKBP51

Plasmid

#89132

Purpose

mammalian expression of FKBP5 also referred to as FKBP51 with N-terminal GAL4 DNA binding domain

Depositor

Elizabeth Wilson

Article

Su et al J Biol Chem. 2012 Oct 5;287(41):34809-24

Insert

FKBP5 (FKBP5 Human)

Tags

GAL4 DBD

Expression

Mammalian

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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prk5-MFSD12-TEV-3xFLAG-3xHA

Plasmid

#149504

Purpose

Transient expression of human MFSD12 with recoded nucleotide sequence.

Depositor

David Sabatini

Article

Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

Insert

MFSD12 (MFSD12 Human)

Use

Transient expression

Tags

TEV-3XHA-3XFLAG

Expression

Mammalian

Mutation

Codon Optimized Sequence

Promoter

CMV

Available Since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pET41a-MED19

Plasmid

#15430

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

MED19 (Med19 Mouse)

Tags

GST and His

Expression

Bacterial

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only