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pCDNA3-HA-Akt1-K179M

Plasmid

#73409

Purpose

Expresses kinase-inactive mutant of Akt with HA tag

Depositor

Jie Chen

Article

Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

Insert

AKT1 (AKT1 Human)

Tags

HA tag

Expression

Mammalian

Mutation

changed K179M to make Kinase inactive mutant

Promoter

CMV

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR221 UBR5

Plasmid

#81062

Purpose

Gateway entry vector encoding full length UBR5

Depositor

Darren Saunders

Article

Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

Insert

Ubiquitin protein ligase E3 component N-recognin 5 (UBR5) (UBR5 Human)

Use

Cloning

Mutation

K503R

Promoter

N/A

Available Since

Jan. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pTT3 Unc5D-FLAG

Plasmid

#72197

Purpose

Expresses full-length Unc5d with a C-terminal FLAG tag

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Unc5d (Unc5d Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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enDR3-TEV-mEGFP-NLS (pRS1451)

Plasmid

#240546

Purpose

Expression of the engineered N-terminal hybrid-binding domain of RNase H3 (enDR3) for capturing R-loops. A TEV cleavage site, mEGFP and nuclear localization signal (NLS) are included in the fusion.

Depositor

Roman Szczęsny

Article

Jedynak-Slyvka et al Nucleic Acids Res. 2025 Aug 11;53(15):gk

Insert

enDR3

Expression

Mammalian

Mutation

L52M

Available Since

Aug. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-neo-TET

Plasmid

#51286

Purpose

expresses U6 RNA, using its own pol III enhancer sequence and tagged with the self splicing intron neo cassette

Depositor

Astrid Roy-Engel

Article

Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:

Insert

U6 (RNU6-1 Human)

Tags

neoTET cassette with a tetrahymena self-splicing …

Expression

Mammalian

Promoter

U6 upstream enhancer sequence

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

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pEf1a-goldDn29-P2A-GFP

Plasmid

#247153

Purpose

Mammalian expression of a human codon optimized engineered goldDn29 recombinase (efficient and specific variant)

Depositor

Patrick Hsu

Article

Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

Insert

goldDn29-P2A-GFP

Tags

6xHis

Expression

Mammalian

Mutation

M6I/E70G/A224P/G227V/Q332K/N341K/L393P/D503N

Promoter

Ef1a

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_NEmut

Plasmid

#123310

Purpose

Expresses the genetically-encoded fluorescent norepinephrine(NE) control sensor GRAB_NEmut in neurons

Depositor

Yulong Li

Article

Feng et al Neuron. 2019 May 22;102(4):745-761.e8. d

Has Service

AAV Retrograde and AAV9

Insert

GPCR activation based NE control sensor GRAB_NEmut

Use

AAV

Promoter

hSyn

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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SpRYc

Plasmid

#175575

Purpose

Expresses the codon-optimized, engineered SpRYc Cas9 nuclease in mammalian cells

Depositor

Joseph Jacobson

Article

Koseki et al Res Sq. 2023 Mar 7:rs.3.rs-2625838. doi:

Insert

SpRYc

Tags

EGFP

Expression

Mammalian

Mutation

PID of SpRY grafted onto the N-terminal domain of…

Promoter

CMV

Available Since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-Myc CDC4 WT*

Plasmid

#16652

Depositor

Bert Vogelstein

Article

Rajagopalan et al Nature. 2004 Mar 4. 428(6978):77-81.

Insert

CDC4 (FBXW7 Human)

Tags

myc

Expression

Mammalian

Mutation

*Note that this insert is only WT relative to the…

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

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pGL3 Zscan4 promoter

Plasmid

#69249

Purpose

Luciferase reporter for Dux4 activation of Zscan4 transcription

Depositor

Stephen Tapscott

Article

Geng et al Dev Cell. 2012 Jan 17;22(1):38-51. doi:

Insert

Zscan4 (ZSCAN4 Human)

Use

Luciferase

Mutation

The two single nucleotide mismatches found betwee…

Promoter

Zscan4

Available Since

Sept. 21, 2015

Availability

Academic Institutions and Nonprofits only

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HP-Cas9hGem-Ade2-LEU2-LINEAR

Plasmid

#174839

Purpose

pRS414 backbone containing LINEAR fragment targeting ADE2 in H. polymorpha

Depositor

Zengyi Shao

Article

Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

Insert

HH-gRNA-HDV targetting ADE2 in Ogataea (para)polymorpha

Use

CRISPR and Synthetic Biology

Expression

Bacterial and Yeast

Promoter

TDH3p

Available Since

Jan. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pTT3 Unc5C-FLAG

Plasmid

#72196

Purpose

Expresses full-length Unc5c with a C-terminal FLAG tag

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Unc5c (Unc5c Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pTT3 Unc5B-FLAG

Plasmid

#72195

Purpose

Expresses full-length Unc5b with a C-terminal FLAG tag

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Unc5b (Unc5b Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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SOX17-3'gRNA

Plasmid

#210465

Purpose

gRNA targeting SOX17 3' terminal for CRISPR-Cas9-mediated knock-in

Depositor

Kai Wang

Article

Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

Insert

SOX17 (SOX17 Human)

Use

CRISPR

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP_sfCherry2(11)_Clathrin light chain

Plasmid

#83032

Purpose

Expresses sfCherry2(11) tagged clathrin light chain in mammalian cells

Depositor

Bo Huang

Article

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

sfCherry2(11)_Clathrin light chain (CLTA Synthetic, Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2017

Availability

Academic Institutions and Nonprofits only

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pCR-II-TOPO-mitfa_LexPR-2A-Cerulean-SV40pA-FRT-Kan-FRT: Sequences,

Plasmid

#110201

Purpose

Melanocyte-specific expression of the LexPR-2A-Cerulean fusion

Depositor

Tatjana Sauka-Spengler

Article

Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

Insert

LexPR transactivator - 2A - Cerulean

Tags

Cerulean

Expression

Bacterial

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pTT3 Unc5A-FLAG

Plasmid

#72194

Purpose

Expresses full-length Unc5a with a C-terminal FLAG tag

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Unc5a (Unc5a Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only

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TRRAP sgRNA3

Plasmid

#138184

Purpose

3rd generation lentiviral gRNA plasmid targeting human TRRAP

Depositor

Wen Xue

Article

Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

Insert

TRRAP (TRRAP Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pFBD-Akt13C(S473D)

Plasmid

#86577

Purpose

Expresses cleavable human full-length Akt1(S473D) phosphomimetic

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt1 (AKT1 Human)

Tags

10xHis

Expression

Insect

Mutation

3C cleavage site inserted at pos. 130; S473D phos…

Available Since

April 4, 2017

Availability

Academic Institutions and Nonprofits only

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pFlare9A-Plekha5E10 WT

Plasmid

#209576

Purpose

minigene construct for Plekha5 E10 alternative splicing

Depositor

Sika Zheng

Article

Zhang et al Neuron. 2019 Feb 20;101(4):690-706.e10.

Insert

Plekha5 (Plekha5 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

March 7, 2024

Availability

Academic Institutions and Nonprofits only

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