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pBS-KS-attB2-SA(0)-T2A-QF2-Hsp70

Plasmid

#62944

Purpose

Creating QF2 drivers from MiMIC lines with inserts into coding introns in Phase 0

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-QF2-Hsp70

Available Since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV-14-Mfn1-T562A

Plasmid

#191976

Purpose

To produce mutants mimicking dephosphorylated Mfn1

Depositor

Luca Scorrano

Article

Pyakurel et al Mol Cell. 2015 Apr 16;58(2):244-54. doi:

Insert

Mfn1 T562A (Mfn1 Mouse)

Expression

Mammalian

Mutation

T562A

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV-14-Mfn1-T562D

Plasmid

#191977

Purpose

To produce mutants mimicking phosphorylated Mfn1

Depositor

Luca Scorrano

Article

Pyakurel et al Mol Cell. 2015 Apr 16;58(2):244-54. doi:

Insert

Mfn1 T562D (Mfn1 Mouse)

Expression

Mammalian

Mutation

T562D

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO-hBFP-2A-RVG-WPRE

Plasmid

#120268

Purpose

Permits retrograde transsynaptic spread by driving Cre-dependent co-expression of the RbG plus a nuclear reporter

Depositor

Edward Callaway

Article

Faget et al Cell Rep. 2016 Jun 21;15(12):2796-808. d

Insert

EF1α-DIO-H2B-tagBFP-Flagx3-T2Am-cB19G

Use

AAV

Expression

Mammalian

Available Since

March 6, 2019

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(2)-T2A-QF2-Hsp70

Plasmid

#62946

Purpose

Creating QF2 drivers from MiMIC lines with inserts into coding introns in Phase 2

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-QF2-Hsp70

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pFKBPmCherry-Akt2(K14A)

Plasmid

#86597

Purpose

Expresses recruitable mCherry-tagged human Akt2(K14A) membrane-binding mutant

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

FKBB-mCherry-Akt2(K14A) (AKT2 )

Expression

Mammalian

Available Since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-Akt2(K14A/R25E)

Plasmid

#86595

Purpose

Expresses eGFP-tagged human Akt2(K14A/R25E) membrane-binding mutant

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt2(K14A/R25E) (AKT2 )

Expression

Mammalian

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pZac2.1 GfaABC1D ChR2(H134R) mCherry SV40

Plasmid

#112496

Purpose

Expresses channel rhodopsin in astrocytes

Depositor

Baljit Khakh

Article

Octeau et al Cell Rep. 2019 May 21;27(8):2249-2261.e7

Insert

ChR2(H134R)

Use

AAV

Tags

mCherry

Mutation

H134R

Promoter

GfaABC1D

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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p3E-GBP

Plasmid

#67672

Purpose

Multisite gateway entry clone for adding C-terminal fusions of GFP-binding nanobody (GBP)

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

anti-GFP nanobody

Use

Gateway multisite 3' entry clone

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(1)-T2A-LexA::GADfluw-Hsp70

Plasmid

#78306

Purpose

Creating LexA::GADfluw lines from MiMIC lines with inserts into coding introns in Phase 1

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-nlsLexA::GADfluw-Hsp70

Available Since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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pSR29

Plasmid

#69255

Purpose

pelt-2::CRE for integration on ttTi14024, Chr X

Depositor

Sander van den Heuvel

Article

Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

Insert

Cre recombinase

Use

Cre/Lox; Mossci

Expression

Worm

Mutation

codon-optimzed index 1.0, tbb-2 UTR is in wrong o…

Promoter

elt-2

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_SNIPR_synRobo/Notch

Plasmid

#188341

Purpose

Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a Robo1-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

Depositor

Kole Roybal

Article

Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

Insert

PGK_antiCD19_Robo1-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64

Use

Lentiviral

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-V5-NLGN2

Plasmid

#82538

Purpose

pCAG vector for expressing V5-NLGN2

Depositor

Alice Ting

Article

Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

Insert

NLGN2 (Nlgn2 Rat)

Tags

V5

Expression

Mammalian

Promoter

CAG

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only
pEGFP-Akt2(T309A)

Plasmid

#86625

Purpose

Expresses eGFP-tagged human Akt2(T309A) phosphomutant

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt2(T309A) (AKT2 Human)

Expression

Mammalian

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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optoPKAW196R

Plasmid

#104548

Purpose

Expresses Cry2-mCherry-PKA W196R in mammalian cells

Depositor

David Lawrence

Article

O'Banion et al Cell Chem Biol. 2017 Oct 25. pii: S2451-

Insert

Cry2-mCherry-PKA W196R

Tags

mCherry

Expression

Mammalian

Mutation

PKA W196R

Promoter

CMV

Available Since

March 12, 2018

Availability

Academic Institutions and Nonprofits only

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pSicoR-shS6K1/2

Plasmid

#81088

Purpose

expresses an shRNA targeting both S6K1 and S6K2 (mouse)

Depositor

Angelique Bordey

Article

Hartman et al Cell Rep. 2013 Oct 31;5(2):433-44. doi:

Insert

S6K1 & S6K2 shRNA

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Promoter

U6

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pILL5-NLS-3xmCitrine

Plasmid

#192525

Purpose

Fluorescent reporter for plant responses to bacterial infection

Depositor

Gitta Coaker

Article

Zhu et al Cell Rep. 2023 Jun 20;42(7):112676. doi:

Insert

IAA-leu-resistant-like5 (ILL5 Mustard Weed)

Tags

3xmCitrine and NLS

Expression

Plant

Promoter

ILL5

Available Since

June 23, 2023

Availability

Academic Institutions and Nonprofits only

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∆C' terminal-DPYSL2-pLJC2

Plasmid

#189867

Purpose

Expresses human DPYSL2 (CRMP2) lacking the C' terminus (491-572 aa), in mammalian cells

Depositor

Yoav Shaul

Article

Abu Rmaileh et al J Cell Biol. 2022 Jul 4;221(7). pii: 213

Insert

ΔC-DPYSL2

Use

Lentiviral

Tags

3X FLAG

Expression

Mammalian

Mutation

deleted the C'terminus of DPYSL2 (491-572 aa)

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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MSCVpic2blast-EGFP-FF2

Plasmid

#164923

Purpose

Expresses EGFP cDNA and FF2 shRNA in mammalian cells

Depositor

Ji Luo

Article

Smith et al Cell Biosci. 2016 Nov 11;6:57. doi: 10.1

Inserts

Enhanced green fluorescent protein
Neuropeptide FF receptor 2 (NPFFR2 Human)

Use

Retroviral

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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aL-mEos3.2

Plasmid

#113154

Purpose

constrained LFA-1-mEos3.2 fusion

Depositor

Timothy Springer

Article

Moore et al Cell Rep. 2018 Feb 13;22(7):1903-1912. d

Insert

full-length human aL with mEOS3.2 in betapropeller (ITGAL Human)

Tags

mEOS3.2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 16, 2018

Availability

Academic Institutions and Nonprofits only

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