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pJW1504

Plasmid

#62358

Purpose

RPS2 HA-TEV-AVI pop-in/pop-out plasmid

Depositor

Jonathan Weissman

Article

Jan et al Science. 2014 Nov 7;346(6210):1257521. d

Insert

RPS2 fragment

Use

Yeast targeting

Tags

HA-TEV-AVI

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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pFF746

Plasmid

#61451

Purpose

aTc-inducible SCRIBE_galK(ON)

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

SCRIBE_galK(ON)

Use

Synthetic Biology

Promoter

pTetO

Available Since

March 6, 2015

Availability

Academic Institutions and Nonprofits only

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pGB-08-09-BECN1

Plasmid

#187991

Purpose

Plasmid for the protein expression of BECN1/Beclin1 in a bacterial and insect expression system. Internal_Reference: SMC1325

Depositor

Sascha Martens

Insert

BECN1 (BECN1 Human)

Expression

Bacterial and Insect

Available Since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pQE80_T5_P3-ehaA_LacI

Plasmid

#186315

Purpose

Plasmid for inducible E coli expression of the P3 helixCAM.

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Insert

P3-ehaA

Expression

Bacterial

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pQE80_T5_AP4-ehaA_LacI

Plasmid

#186316

Purpose

Plasmid for inducible E coli expression of the AP4 helixCAM.

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Insert

AP4-ehaA

Expression

Bacterial

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pmCherry-Dr-TrkA

Plasmid

#127963

Purpose

Myr mCherryDrTrkA expression in mammalian cells

Depositor

Vladislav Verkhusha

Article

Leopold et al Nat Commun. 2019 Mar 8;10(1):1129. doi:

Insert

Myr-mCherry-3xSAG-Dr-hel-4- TrkA

Expression

Mammalian

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pYPQ138A

Plasmid

#69280

Purpose

Golden Gate entry vector; 8th gRNA under AtU6 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pYPQ137A

Plasmid

#69279

Purpose

Golden Gate entry vector;7th gRNA under AtU6 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pCI-neo-μ4-(HA)3

Plasmid

#198180

Purpose

Expression of HA-tagged AP-4 μ4 in mammalian cells

Depositor

Juan Bonifacino

Article

Mattera et al PLoS Pathog. 2014 May 15;10(5):e1004107.

Insert

AP-4 μ4

Tags

triple HA tag

Expression

Mammalian

Promoter

CMV

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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366 pTol2-CherryDest

Plasmid

#73487

Purpose

promoterless Tol2 vector with mCherry tag upstream of and in frame with attR1/attR2 cassette and late SV40 polyadenylation signal

Depositor

Nathan Lawson

Article

Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

Insert

mCherry-attR1/attR2

Mutation

H30R (please see depositor comments below)

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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pJB042

Plasmid

#22618

Depositor

Lynn Hudson

Article

Berndt et al J Biol Chem. 1992 Jul 25. 267(21):14730-

Insert

Human genomic PLP promoter region (PLP1 Human)

Use

Cat reporter plasmid

Available Since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only
M24 Nac>Nac

Plasmid

#17174

Depositor

Randall Moon

Article

Dorsky et al Genes Dev. 2000 Jan 15;14(2):158-62.

Insert

nacre promoter driving nacre cDNA (mitfa Zebrafish)

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pDA12

Plasmid

#113663

Purpose

Cloning vector for bacterial expression with Pdhfr promoter

Depositor

Miguel Valvano

Article

Aubert et al Infect Immun. 2008 May;76(5):1979-91. do

Type

Empty backbone

Expression

Bacterial

Promoter

Pdhfr

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pCS2+ MT Beta Catenin (Xenopus)

Plasmid

#13435

Depositor

Marc Kirschner

Article

Lee et al J Cell Biol. 2001 Sep 3. 154(5):983-93.

Insert

Beta Catenin (ctnnb1.L Frog)

Tags

Myc Tag

Expression

Mammalian

Available Since

Nov. 1, 2006

Availability

Academic Institutions and Nonprofits only
PM-TD!TB

Plasmid

#48656

Purpose

Bacterial TD crRNA expression: targets TD to protospacer B (ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicol

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial TD crRNA to prototspacer B

Use

CRISPR

Promoter

J23100 promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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EB3-iRFP713

Plasmid

#128007

Purpose

In vivo visualization of microtubule plus ends with near-infrared

Depositor

Dorus Gadella

Article

Van Geel et al Sci Rep. 2020 Apr 7;10(1):6034. doi: 10.

Insert

EB3-iRFP713

Expression

Mammalian

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pBABE Rad

Plasmid

#11213

Depositor

Ronald Kahn

Article

Zhu et al J Biol Chem. 1996 Jan 12. 271(2):768-73.

Insert

Rad (RRAD Human)

Use

Retroviral

Expression

Mammalian

Available Since

March 1, 2006

Availability

Academic Institutions and Nonprofits only
pFSy(336)GW

Plasmid

#27231

Depositor

Pavel Osten

Article

Dittgen et al Proc Natl Acad Sci U S A. 2004 Dec 28. 1

Insert

EGFP

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Available Since

March 11, 2011

Availability

Academic Institutions and Nonprofits only

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pENTR-L1-CAMKII(0.4)-L4

Plasmid

#32579

Depositor

Matthew Nolan

Article

White et al Front Mol Neurosci. 2011;4:8. Epub 2011

Insert

CAMKIIalpha promoter 0.4kb version

Use

Gateway cloning vector

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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kermit/pCS2+

Plasmid

#16309

Depositor

Peter Klein

Article

Tan et al Development. 2001 Oct . 128(19):3665-74.

Insert

Kermit (gipc1 Frog)

Use

Vertebrate expression

Available Since

Dec. 21, 2007

Availability

Academic Institutions and Nonprofits only

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pProm11_BCD1-GFP

Plasmid

#80656

Purpose

Promoter11 with BCD1-gfp

Depositor

Nathan Hillson

Article

Linshiz et al ACS Synth Biol. 2014 Aug 15;3(8):515-24.

Insert

GFP

Use

P15a origin, constitutive promoter, kanr

Mutation

NONE

Promoter

constitutive promoter

Available Since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pEG3a

Plasmid

#31398

Depositor

Kenn Gerdes

Article

Ebersbach et al Mol Microbiol. 2008 May . 68(3):720-35.

Insert

zapB (zapB E. coli)

Tags

GFP

Expression

Bacterial

Available Since

Sept. 15, 2011

Availability

Academic Institutions and Nonprofits only
LHP585 - Ub-CYC1

Plasmid

#32168

Depositor

Linda Hicke

Article

Shih et al EMBO J. 2003 Mar 17;22(6):1273-81.

Insert

uba1 yeast (UBA1, UBA2 Budding Yeast)

Expression

Yeast

Promoter

CUP1

Available Since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
MIP-T3

Plasmid

#99511

Purpose

to express MIP-T3 in mammalian system

Depositor

Dong-Yan Jin

Article

Ng et al J Immunol. 2011 Dec 15;187(12):6473-82.

Insert

MIP-T3 (TRAF3IP1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pLLU2G-Oct3

Plasmid

#21616

Depositor

David Garbers

Article

Dann et al Stem Cells. 2008 Nov . 26(11):2928-37.

Insert

Oct3 shRNA

Use

Lentiviral and RNAi

Available Since

Aug. 25, 2009

Availability

Academic Institutions and Nonprofits only

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pWZ414-F13

Plasmid

#23056

Depositor

Sharon Dent

Article

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Expression

Yeast

Available Since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only
maturase-RT

Plasmid

#181805

Purpose

E.r. maturase RT

Depositor

Erik Sontheimer

Article

Liu et al Nat Biotechnol. 2022 Apr 4. pii: 10.1038

Insert

maturase-RT

Expression

Mammalian

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pIBG-FTet

Plasmid

#33361

Depositor

Doug Mortlock

Article

Chandler et al Mol Cell Biol. 2007 Apr;27(8):2934-51. E

Inserts

IRES-Bgeo
TetR cassette

Use

Mouse Targeting

Tags

FRT site

Available Since

Feb. 22, 2012

Availability

Academic Institutions and Nonprofits only

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pEIAV-iSyn-eGFPW

Plasmid

#80161

Purpose

Expression in mammalian cells

Depositor

Edward Callaway

Article

Cetin et al J Neurophysiol. 2014 May;111(10):2150-9.

Insert

eGFP

Use

Lentiviral

Promoter

iSyn

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pRS416-ACT1_intron

Plasmid

#127602

Purpose

low-copy URA-selectable plasmid, ACT1 intron inserted into URA3

Depositor

David Bartel

Article

Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

Insert

ACT1 intron

Expression

Yeast

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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