We narrowed to 14,497 results for: SHR

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pHR: pmU6 sgChr13q34 2xMS2 pUbC MCP-mCherry-p2a-Puro

Plasmid

#174119

Purpose

Expression of sgRNA targeting Chr13q34 (chr13: 112277011 - 112319170, hg38)

Depositor

Stanley Qi

Article

Gao et al Mol Cell. 2021 Aug 13. pii: S1097-2765(2

Insert

sgChr13q34 2xMS2 and MCP-mCherry-p2a-Puro

Use

Lentiviral

Promoter

mU6 and UbC

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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Keratin 8 S431A

Plasmid

#18058

Depositor

M Bishr Omary

Article

Ku et al J Biol Chem. 2002 Mar 29. 277(13):10775-

Insert

Keratin 8 (KRT8 Human)

Expression

Mammalian

Mutation

S431A

Available Since

May 16, 2008

Availability

Academic Institutions and Nonprofits only
pBXMCS-2 Pconstitutive-sgRNA(Spy)_ctrA

Plasmid

#133334

Purpose

for constitutive expression of a single guide RNA from Streptococcus pyogenes with a seed region that targets ctrA gene's promoter from Caulobacter crescentus

Depositor

Michael Laub

Article

Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

Insert

sgRNA_ctrA

Use

CRISPR

Promoter

constitutive

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pSR9.924

Plasmid

#39539

Depositor

Katharina Strub

Article

Mary et al RNA. 2010 May;16(5):969-79. Epub 2010 Ma

Insert

SRP9 (SRP9 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

H1

Available Since

Sept. 10, 2012

Availability

Academic Institutions and Nonprofits only
pOC4 Actb-mRuby3 KI

Plasmid

#183437

Purpose

FlpON knock-in for mRuby3-beta-actin (amino acid position: before start codon)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and mRuby3 donor

Use

CRISPR

Expression

Mammalian

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pOC5 Tubb3-GFP KI

Plasmid

#183440

Purpose

FlpOFF knock-in for beta3-tubulin GFP (amino acid position: stop codon)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and GFP donor

Use

CRISPR

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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SGL40C.EFS.RFP657

Plasmid

#69147

Purpose

Advanced lentiviral Vector for sgRNA (hU6 Promoter) delivery with RFP657 expression (EFS Promoter)

Depositor

Dirk Heckl

Article

Reimer et al Haematologica. 2017 Jun 1. pii: haematol

Inserts

sgRNA
EFS
tagRFP657

Available Since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pDGE5

Plasmid

#79461

Purpose

sgRNA shuttle vector; module 1

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

pAtU6-ccdB-sgRNA (ccdB(letD) )

Use

CRISPR and Synthetic Biology

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pCXLE-dCas9GFP-shP53

Plasmid

#102906

Purpose

EBNA episome plasmid for CAG promoter constitutive expression of dCas9-GFP. Includes p53 shRNA expression cassette.

Depositor

Timo Otonkoski

Article

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

dCas9GFP-shP53

Use

CRISPR

Expression

Mammalian

Mutation

D10A, H840A

Promoter

CAG

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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pAN-PBAD-sgRNA-scramble

Plasmid

#62285

Purpose

expression of scramble sgRNA from the arabinose-inducible promoter

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

scramble sgRNA

Use

CRISPR

Expression

Bacterial

Promoter

pBAD

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pCRRNA-G0-R0

Plasmid

#101803

Purpose

Repress mCherry and GFP independently, with different strengths

Depositor

David Bikard, Sven van Teeffelen

Article

Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

Insert

crRNAs targeting GFP and mCherry

Use

CRISPR and Synthetic Biology

Promoter

Spy 1049

Available Since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pG3H-U6EC1

Plasmid

#134758

Purpose

pGreen3 CRISPR/Cas9

Depositor

Qi-Jun Chen

Article

Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

Insert

CRISPR/Cas9

Use

CRISPR

Promoter

gRNA-U6, zCas9-EC1

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pDYSCKO

Plasmid

#120263

Purpose

Empty backbone for cloning any gRNA to be used with the canavanine co-selection system

Depositor

Christian Landry

Article

Despres et al G3 (Bethesda). 2018 Oct 3;8(10):3163-317

Insert

DYSCKO cassette

Use

CRISPR and Synthetic Biology

Promoter

SNR52

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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double_sgRNA targeting e3 and e7

Plasmid

#190689

Purpose

sgRNAs targeting enhancer 3 and 7 of MYC separately

Depositor

Stanley Qi

Article

Lin et al Science. 2022 Aug 11:eabk3512. doi: 10.1

Insert

sgRNAs targeting enhancer 3 and 7 of MYC separately

Use

CRISPR, Lentiviral, and Synthetic Biology

Tags

BFP and Puromycin

Expression

Mammalian

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pSR9.1347

Plasmid

#39540

Depositor

Katharina Strub

Article

Mary et al RNA. 2010 May;16(5):969-79. Epub 2010 Ma

Insert

SRP9 (SRP9 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Sept. 10, 2012

Availability

Academic Institutions and Nonprofits only
CROP_C9_Puro_FGFR1

Plasmid

#183289

Purpose

All-in-One CRISPRko system with a guide RNA that targets FGFR1 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

FGFR1

Use

Lentiviral

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_FGFR2

Plasmid

#183290

Purpose

All-in-One CRISPRko system with a guide RNA that targets FGFR2 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

FGFR2

Use

Lentiviral

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pEE389

Plasmid

#149287

Purpose

T-DNA encoding TRV2 with Truncated FT augmented gRNA targeting NbAG

Depositor

Daniel Voytas

Article

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Insert

p35S:TRV2_NbAGsgRNA1_TrunFT:tNos

Expression

Plant

Promoter

35S

Available Since

July 20, 2020

Availability

Academic Institutions and Nonprofits only
pEE387

Plasmid

#149285

Purpose

T-DNA encoding TRV2 with FT augmented gRNA targeting NbAG

Depositor

Daniel Voytas

Article

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Insert

p35S:TRV2_NbAGsgRNA1_FT:tNos

Expression

Plant

Promoter

35S

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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PB-HD118

Plasmid

#172655

Purpose

Expressing paired pegRNAs from human U6 and H1 promoters to make 118-bp deletion on HPRT1 gene

Depositor

Jay Shendure

Article

Choi et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

Insert

pegRNA-HD118A/pegRNA-HD118B

Use

CRISPR

Available Since

Oct. 22, 2021

Availability

Academic Institutions and Nonprofits only

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