We narrowed to 41,438 results for: lat

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pDGB3_alpha2_4xCBS-mDFR-Rep-RepA-tNOS (GB3625)

Plasmid

#225433

Purpose

Transcriptional unit for copper-regulation of BeYDV Rep/RepA with 4xCBS domain+minimal promoter DFR for induction by copper.

Depositor

Diego Orzaez

Article

Garcia-Perez et al Plant Biotechnol J. 2025 Jan;23(1):141-1

Insert

4xCBS:mDFR:Rep/RepA:Tnos

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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FKBP-alpha(740-977)-GFP

Plasmid

#100731

Purpose

To express a clathrin "hook" in mammalian cells to hot-wire endocytosis (negative control)

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

AP2A1 (Ap2a1 Mouse)

Tags

FKBP and GFP

Expression

Mammalian

Mutation

Long isoform

Promoter

CMV

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pCD564

Plasmid

#153026

Purpose

dxCas9(3.7), MCP-SoxS(R93A/S101A)

Depositor

Jesse Zalatan

Article

Fontana et al Nat Commun. 2020 Apr 1;11(1):1618. doi:

Insert

dxCas9(3.7), MCP-SoxS(R93A/S101A)

Use

CRISPR

Expression

Bacterial

Mutation

SoxS has R93A and S101A mutations

Available Since

Aug. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pET27b-ecDHFR-N23PP/L28F

Plasmid

#118584

Purpose

Bacterial expression of E.coli DHFR N23PP and L28F mutant

Depositor

Stephen Benkovic

Article

Liu et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

Insert

DHFR

Expression

Bacterial

Mutation

L28 mutated to F28, N23 mutated to P23, a proline…

Promoter

T7

Available Since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pEx1-pEF-H2B-mCherry-T2A-rTetR-KRAB-Zeo

Plasmid

#78352

Purpose

Expresses rTetR-KRAB for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.

Depositor

Michael Elowitz

Article

Bintu et al Science. 2016 Feb 12;351(6274):720-4. do

Insert

H2B-mCherry-T2A-rTetR-KRAB

Use

Synthetic Biology

Tags

rTetR

Expression

Mammalian

Promoter

pEF alpha

Available Since

July 7, 2016

Availability

Academic Institutions and Nonprofits only

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pLL3.7m_tKiMBImut-T2A-AkaLuc-P2A-Blasticidin

Plasmid

#199585

Purpose

Express tKiMBImut(AA), AkaLuc and Blasticidin in a lentivirus vector

Depositor

Michael Lin

Article

Wu et al ACS Cent Sci. 2023 Mar 20;9(4):719-732.

Inserts

ERK tdTomato-Kinase-Modulated Bioluminescent Indicator(mutant)
AkaLuc
Blasticidin

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

June 23, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-dCas9-MQ1(S317A)-EGFP

Plasmid

#89636

Purpose

Targeted CpG methylation

Depositor

Margaret Goodell

Article

Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

Insert

site-specific DNA-methyltransferase SssI

Use

CRISPR

Tags

3*FLAG and 6*His

Expression

Mammalian

Mutation

S317A, AGC-->GCC

Promoter

CMV

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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DHFR-GFP-th-NL1

Plasmid

#173007

Purpose

For light regulated release of neuroligin 1 from the endoplasmic reticulum. Driven by synapsin promoter. Thrombin site is included between GFP and NL1.

Depositor

Matthew Kennedy

Article

Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

Insert

DHFR-GFP-NL1

Tags

DHFR and EGFP

Expression

Mammalian

Promoter

human synapsin

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pHM123DEnv

Plasmid

#137668

Purpose

Express HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)

Depositor

Anjali Joshi

Article

Joshi et al Virology. 2017 Dec;512:222-233. doi: 10.

Insert

HIV-1 Envelope Glycoprotein

Expression

Mammalian

Available Since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV6- Entry-RPL26DC-DDK

Plasmid

#133867

Purpose

Expression of C-terminally FLAG-tagged human RPL26 mutant lacking the C-terminal UFMylation sites

Depositor

Yihong Ye

Article

Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

Insert

RPL26DC-myc-FLAG (RPL26 Human)

Tags

Myc-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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DHFR-GFP-th-GluA1

Plasmid

#173008

Purpose

For light regulated release of GluA1 from the endoplasmic reticulum. Driven by synapsin promoter. Thrombin site is included between GFP and GluA1.

Depositor

Matthew Kennedy

Article

Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

Insert

DHFR-GFP-th-GluA1

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

human synapsin

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pFAB3677

Plasmid

#47833

Purpose

BIOFAB RFP reporter plasmid for measuring promoter 2 + BCD1 efficiency.

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

promoter 2 and BCD1

Use

Synthetic Biology

Expression

Bacterial

Promoter

see insert

Available Since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pLJM1 p18 mito

Plasmid

#26638

Depositor

David Sabatini

Article

Sancak et al Cell. 2010 Apr 16. 141(2):290-303.

Insert

p18 mito (LAMTOR1 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Available Since

Oct. 26, 2010

Availability

Academic Institutions and Nonprofits only
pXPR_BRD003 sgKEAP1-2

Plasmid

#74986

Purpose

sgKEAP1-2, puromycin selection

Depositor

William Hahn

Article

Krall et al Elife. 2017 Feb 1;6:e18970. doi: 10.7554

Insert

KEAP1

Use

CRISPR

Expression

Mammalian

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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N-BLa 1.1

Plasmid

#88994

Purpose

BLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.1 fusionprotein containing the N-terminal fragment of the split β-lactamase.

Depositor

Dieter Langosch

Article

Schanzenbach et al Sci Rep. 2017 Mar 7;7:43476. doi: 10.103

Insert

N-BLa 1.1 fusion protein

Tags

Flag

Expression

Bacterial

Promoter

araBAD

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RIPK3[S227D]-FLAG

Plasmid

#199320

Purpose

mutated phosphorylation site (MLKL interaction permanent active)

Depositor

Adrian Press

Article

Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

Insert

Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed Serine 227 to Aspartic Acid

Available Since

May 25, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RIPK3[S227A]-FLAG

Plasmid

#199318

Purpose

mutated phosphorylation site (MLKL interaction inhibited)

Depositor

Adrian Press

Article

Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

Insert

Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed Serine 227 to Alanine

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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LITE1.0_pAAV_hSyn_CRY2PHR-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA

Plasmid

#47454

Purpose

epiLITE / LITE1.0 CRY2PHR fused to SID4X transcriptional repressor effector domain. Binds to CIB1 upon blue light stimulation. Synapsin promoter for neuronal expression.

Depositor

Feng Zhang

Article

Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

Insert

CRY2PHR-NLS-SID4X

Use

AAV

Tags

2A_phiLOV2.1

Expression

Mammalian

Promoter

hSyn human synapsin (mammalian neuronal expressio…

Available Since

Oct. 15, 2013

Availability

Academic Institutions and Nonprofits only

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EYFP-NLS-actin-K68/284R

Plasmid

#60616

Purpose

Encodes YFP and NLS tagged beta-actin with sumoylation resistant mutations

Depositor

Primal de Lanerolle

Article

Hofmann et al J Cell Biol. 2009 Jul 27;186(2):193-200.

Insert

Beta-actin (ACTB Human)

Tags

EYFP and NLS

Expression

Mammalian

Mutation

K68R, 284R

Available Since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only
pCI-FLAG-mRRP6

Plasmid

#60045

Purpose

Mammalian expression of FLAG-tagged mouse RRP6

Depositor

Zissimos Mourelatos

Article

Liu et al Mol Cell. 2014 Aug 27. pii: S1097-2765(1

Insert

RRP6 (Exosc10 Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 20, 2014

Availability

Academic Institutions and Nonprofits only