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pBT372

Plasmid

#125613

Purpose

Expresses evoCDA1-BE4max-NG in mammalian cells

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

evoCDA1-BE4max-NG

Use

Tags

Expression

Mammalian

Mutation

F23S A123V I195F

Promoter

Available since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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Nb-gp41-GFP (MoonTag-Nb-GFP)

Plasmid

#128602

Purpose

Expresses nanobody-2H10 fused to super folded GFP (sfGFP) in mammalian cells. It recognizes gp41 peptide array (MoonTag peptide array).

Depositor

Marvin Tanenbaum

Article

Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

Insert

Nanobody_gp41

Use

Lentiviral

Tags

GB1 and sfGFP

Expression

Mutation

Promoter

SFFV

Available since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pDD425

Plasmid

#202078

Purpose

Frame +0 selector for NHEJ knock-in

Depositor

Daniel Dickinson

Article

Shi et al Development. 2023 May 15;150(10):dev2013

Insert

pEF1a-mCherry-Cas9 + Frame +0 sgRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

EF1a

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-EGFP-LAMP2

Plasmid

#222321

Purpose

Synchronize the trafficking of LAMP2 from the ER.

Depositor

Franck Perez

Article

Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

Insert

Streptavidin-KDEL and LAMP2 fused to SBP-EGFP (LAMP2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-hSyn1-GFP

Plasmid

#177810

Purpose

Encodes a GFP driven by human synapsin I promoter

Depositor

Christopher Grigsby

Article

Mattiassi et al Biomater Sci. 2021 Aug 7;9(15):5175-5191

Insert

CopGFP inserted behind human synapsin I promoter (SYN1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Syn1

Available since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-GRAB_ACh3.0

Plasmid

#121923

Purpose

express the genetically-encoded fluorescent acethycholine(ACh) sensor GRAB-ACh3.0 in a cre-dependent manner

Depositor

Yulong Li

Article

Jing et al Nat Methods. 2020 Sep 28. pii: 10.1038/s

Insert

GPCR activation based ACh sensor GRAB-ACh3.0

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

Jan. 16, 2020

Availability

Academic Institutions and Nonprofits only

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EF1a-dCas9-KRAB-GFP backbone

Plasmid

#194281

Purpose

Vector for CRISPRi-GFP expression ready for gateway cloning of sgRNA

Depositor

Elizabeth Kirby

Article

Rieskamp et al iScience. 2023 Jun 8;26(7):107068. doi:

Insert

dCas9-KRAB-GFP

Use

CRISPR and Lentiviral

Tags

GFP

Expression

Mutation

Promoter

EF1a

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pRSET-iGluSnFR4s

Plasmid

#234443

Purpose

Bacterial expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow)

Depositor

GENIE Project

Article

Aggarwal et al bioRxiv 2025.03.20.643984

Insert

iGluSnFR4s

Use

Tags

6xHis-Xpress epi

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-GINKO1

Plasmid

#113112

Purpose

Mammalian expression of genetically encoded green fluorescent potassium ion indicator GINKO1

Depositor

Robert Campbell

Article

Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

Insert

GINKO1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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EGFP-COV M

Plasmid

#165124

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

COV M (M codon optimized SARS-COV-2)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pFOK

Plasmid

#166651

Purpose

Suicide vector for gene manipulation

Depositor

Dirk Bumann

Article

Cianfanelli et al BMC Microbiol. 2020 May 24;20(1):129. do

Insert

I-sceI, sacB

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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myc-Rictor corrected

Plasmid

#11367

Purpose

Mammalian expression of myc tagged human RICTOR

Depositor

David Sabatini

Article

Sarbassov et al Curr Biol 2004 Jul 27;14(14):1296-302.

Insert

Rictor (RICTOR Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

April 27, 2006

Availability

Academic Institutions and Nonprofits only

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pDONR207 SARS-CoV-2 ORF3A

Plasmid

#141271

Purpose

Gateway-compatible Entry vector, with insert of ORF3A gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

ORF3A (ORF3a SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway-compatible entry vector

Tags

Expression

Mutation

Many synonymous changes due to codon optimization

Promoter

Available since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pHAGE-FLAG-APEX2-TAX1BP1

Plasmid

#175761

Purpose

Lentiviral vector expressing N-terminally tagged APEX2-FLAG-TAX1BP1

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

TAX1BP1 (TAX1BP1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pJMK074: CMV QuasAr2-TS-GCaMP6f

Plasmid

#72303

Purpose

Combines a gCaMP-based calcium indicator and an Arch-based voltage indicator

Depositor

Adam Cohen

Article

Dempsey et al J Pharmacol Toxicol Methods. 2016 May 13

Insert

CaViar

Use

Lentiviral

Tags

TS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCXLE-EGFP

Plasmid

#27082

Purpose

Integration-free (episomal) expression of EGFP

Depositor

Shinya Yamanaka

Article

Okita et al Nat Methods. 2011 May;8(5):409-12

Insert

eGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 10, 2011

Availability

Academic Institutions and Nonprofits only

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Cyto-roGFP

Plasmid

#49435

Purpose

Expresses thiol redox-sensitive ratiometric sensor roGFP in cytosol of mammalian cells

Depositor

Paul Schumacker

Article

Waypa et al Circ Res. 2010 Feb 19;106(3):526-35. doi

Insert

roGFP2

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

C48S, Q80R, S147C, and Q204C in Clontech's G…

Promoter

CMV

Available since

Dec. 9, 2013

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-CD19.28H.28TM.28.3z

Plasmid

#200678

Purpose

Modular CD28hinge-CD28transmembrane-CD28-CD3z CAR backbone, For Transient Expression or Lentiviral Production

Depositor

Michele Bernasconi

Article

Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

Insert

FMC63-CD28HD-CD28TM-CD28-CD3z-P2A-EGFP (CD28, CD247 Human)

Use

Lentiviral

Tags

MycTag

Expression

Mammalian

Mutation

Promoter

EF1a-short

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pDD427

Plasmid

#202080

Purpose

Frame +2/-1 selector for NHEJ knock-in

Depositor

Daniel Dickinson

Article

Shi et al Development. 2023 May 15;150(10):dev2013

Insert

pEF1a-mCherry-Cas9 + Frame +2/-1 sgRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

EF1a

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TLR8-YFP

Plasmid

#13024

Depositor

Doug Golenbock

Insert

Toll-Like Receptor 8 (TLR8 Human)

Use

Tags

YFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 3, 2006

Availability

Academic Institutions and Nonprofits only

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