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157,954 results

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EGFR-mEGFP

Plasmid

#203774

Purpose

Number and brightness analysis of EGFR oligomerization

Depositor

Thorsten Wohland

Article

Gupta et al J Lipid Res. 2022 Jun;63(6):100220. doi:

Insert

EGFR (EGFR Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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mEos3.2-N1

Plasmid

#54525

Purpose

Localization: N1 Cloning Vector, Excitation: 507 / 572, Emission: 516 / 580

Depositor

Tao Xu, Michael Davidson

Article

Zhang et al Nat Methods. 2012 May 13;9(7):727-9. doi

Type

Empty backbone

Use

Tags

mEos3.2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 12, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pHR_Gal4UAS_human_leucinezipperTRAIL _IRES_mCherry_PGK_tBFP

Plasmid

#85433

Purpose

Lentiviral vector for Gal4 inducible leucinezipper TRAIL with mCherry reporter and constitutive tBFP expression

Depositor

Wendell Lim

Article

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

leucine zipper human TRAIL secreted form

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 23, 2017

Availability

Academic Institutions and Nonprofits only
pCMV5-Smad7-HA

Plasmid

#11733

Depositor

Jeff Wrana

Article

Hayashi et al Cell. 1997 Jun 27. 89(7):1165-73.

Insert

SMAD7 (SMAD7 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

May 12, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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AAV pCAG-FLEX-mScarlet-WPRE

Plasmid

#99280

Purpose

Can be used to generate AAV virus that will express mScarlet in the presence of Cre

Depositor

Rylan Larsen

Insert

mScarlet

Use

AAV and Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

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Str-Golgin84_VSVG-SBP-EGFP

Plasmid

#65305

Purpose

synchronize trafficking of Golgin-84 from the Golgi apparatus (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Golgin-84 (GOLGA5 Human)

Use

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 28, 2015

Availability

Academic Institutions and Nonprofits only

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EF1a_mCherry_P2A_Hygro

Plasmid

#135003

Purpose

Lentiviral vector to express mCherry or to clone and express gene of interest, under control of EF1a promoter. Barcodes compatible with detection in single cell RNA-seq may be inserted at HpaI site.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pAKlux2

Plasmid

#14080

Depositor

Attila Karsi

Article

Karsi et al Plasmid. 2007 May;57(3):286-95. doi: 10.

Insert

Bacterial luciferase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Lac, T3

Available since

March 12, 2007

Availability

Academic Institutions and Nonprofits only

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pBabe-EGFP-Hu_Plk1(WT)

Plasmid

#136323

Purpose

Retroviral expression of human Plk1-WT with N-terminal EGFP

Depositor

Guillermo de Cárcer, Marcos Malumbres

Insert

PLK1 (PLK1 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-3XFLAG-renilla-luciferase-beta-globin-control

Plasmid

#184393

Purpose

Transient transfection plasmid expressing Renilla-luciferase-beta-globin fusion protein

Depositor

Robert Bradley

Article

Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

Insert

Renilla luciferase

Use

Tags

3X FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pSMALB-ATF4.14

Plasmid

#155034

Purpose

Fluorescent reporter for ATF4 translation, Positive Control

Depositor

Peter van Galen, John Dick

Article

van Galen et al Nature. 2014 Jun 12;510(7504):268-72. do

Inserts

ATF4 5' UTR (Atf4 Mouse)
ATF4-eGFP fusion ORF
TagBFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

uORF1 and uORF2 start codons mutated

Promoter

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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mc2 puro

Plasmid

#206215

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Simon Conn, Brett Stringer

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pMGF182

Plasmid

#97006

Purpose

Expresses GFP-CHMP7 in mammalian cells under a crippled promoter

Depositor

Wesley Sundquist, Adam Frost

Article

Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

Insert

CHMP7 (CHMP7 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV delta 5

Available since

Aug. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pRC_RR_VP1_r1c3

Plasmid

#65724

Purpose

Rescued infectious variant from pAAV2_RaPID library; mCherry insertion in place of the deleted amino acid sequence 453-GTTTQSR

Depositor

Junghae Suh

Article

Judd et al Mol Ther Nucleic Acids. 2012 Nov 13;1:e5

Insert

mCherry

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EGFP-RhoA-T19N

Plasmid

#12967

Depositor

Gary Bokoch

Article

Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

Insert

RhoA DN (RHOA Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

T19N Dominant negative

Promoter

Available since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

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pYTK001

Plasmid

#65108

Purpose

Part Plasmid Entry Vector to be used in the Dueber YTK system.

Depositor

John Dueber

Article

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

none

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pcmv3-IRG1-HA

Plasmid

#198181

Purpose

Mammalian expression of HA-tagged human ACOD1 (IRG1)

Depositor

Yun Zhao

Article

Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

Insert

ACOD1 (ACOD1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

HA tag is added to C-terminal of target protein

Promoter

CMV

Available since

April 7, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-APEX2-NIK3x

Plasmid

#129274

Purpose

Expression of APEX2 in the nucleolus

Depositor

Alice Ting

Article

Fazal et al Cell. 2019 Jun 14. pii: S0092-8674(19)30

Insert

GFP-APEX2-NIK3x

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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TFORF0037

Plasmid

#141488

Purpose

Lentiviral vector for overexpressing the SP5 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

SP5 (SP5 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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HLA-A2/K^b

Plasmid

#14906

Depositor

Linda Sherman

Article

Irwin et al J Exp Med. 1989 Oct 1. 170(4):1091-101.

Insert

HLA-A2/K^b (HLA-A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 15, 2007

Availability

Academic Institutions and Nonprofits only

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pHAGE EF1α dCas9-KRAB

Plasmid

#50919

Purpose

Constitutive dCas9-KRAB 2nd generation lentiviral expression vector

Depositor

Rene Maehr, Scot Wolfe

Article

Kearns et al Development. 2014 Jan;141(1):219-23. doi

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

3xHA and KRAB

Expression

Mammalian

Mutation

D10A, H840A

Promoter

EF1alpha

Available since

March 28, 2014

Availability

Academic Institutions and Nonprofits only

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VE-cad-eGFP Donor plasmid

Plasmid

#92309

Purpose

Knock eGFP into human VE-cad locus

Depositor

Sean Palecek

Article

Bao et al Bioeng Transl Med. 2017 Jun;2(2):191-201

Insert

VEcad Homologous Recombination Arms (CDH5 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

about 30 a.a. truncated at end of protein

Promoter

Available since

Aug. 1, 2017

Availability

Academic Institutions and Nonprofits only

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amajLime chromoprotein

Plasmid

#117843

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses amajLime chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, amajLime

Use

Synthetic Biology; Escherichia coli

Tags

Expression

Mutation

BioBrick sites removed

Promoter

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-GFP(-30)

Plasmid

#199167

Purpose

used to express a supercharged GFP variant in E. coli

Depositor

Allie Obermeyer

Article

Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

Insert

GFP(-30)

Use

Tags

MGHHHHHHGGA

Expression

Mutation

T19D, K36E, N49E, K51E, K62E, R83D, K89D, N159D, …

Promoter

T7

Available since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCSIIpuro2-H2B-iRFP

Plasmid

#197352

Purpose

Encoding nuclear-targeted iRFP713.

Depositor

Michiyuki Matsuda

Article

Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

Insert

iRFP with nucleus-targeting sequence

Use

Lentiviral

Tags

H2B (human histone 2B)

Expression

Mammalian

Mutation

Promoter

EF-1α

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pGWB517

Plasmid

#74859

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with 4xMyc (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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3XFlagNICD1

Plasmid

#20183

Depositor

Raphael Kopan

Article

Ong et al J Biol Chem. 2006 Feb 24. 281(8):5106-19

Insert

Notch 1 Intracellular Domain (Notch1 Mouse)

Use

Tags

3XFLAG

Expression

Mammalian

Mutation

Contains murine Notch 1 Intracellular Domain (Val…

Promoter

CMV

Available since

Dec. 19, 2012

Availability

Academic Institutions and Nonprofits only

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p53 (dominant negative R175H mutant)-pcw107-V5

Plasmid

#64638

Purpose

3rd generation lentiviral transfer plasmid. When used to produce lentivirus, express physiological levels of insert

Depositor

Kris Wood, David Sabatini

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

TP53 (transcript variant 3) (TP53 Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

R175H

Promoter

PGK

Available since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only

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DR5rev::erRFP

Plasmid

#61011

Purpose

Auxin signalling reporter (DR5rev) driving RFP (ER-targeted)

Depositor

Alexis Maizel

Article

Marin et al Plant Cell. 2010 Apr;22(4):1104-17. doi:

Insert

DR5rev::erRFP:35S_term

Use

Tags

Expression

Plant

Mutation

Promoter

DR5rev::erRFP:35Sterm

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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puc19-pCas13a

Plasmid

#118963

Purpose

Intermediate/cloning vector that can be used for sub-cloning into final destination vectors based on one's requirements.

Depositor

Magdy Mahfouz

Article

Aman et al Genome Biol. 2018 Jan 4;19(1):1. doi: 10

Insert

Cas13a

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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