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Plasmid#60135PurposeRed beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by MET17 promoter.DepositorInsertRdLuc
UseTagsClpXP degron and Nuclear localization signal (NLS…ExpressionYeastMutationPromoterMET17prAvailable sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNB753
Plasmid#60148PurposeGreen beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by LEU1 promoter.DepositorInsertGrLuc
UseTagsClpXP degron and Nuclear localization signal (NLS…ExpressionYeastMutationPromoterLEU1prAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
mPapaya-LC-Myosin-C-10
Plasmid#56398PurposeLocalization: Light Chain Myosin, Excitation: 530, Emission: 541DepositorInsertLC-Myosin
UseTagsmPapayaExpressionMammalianMutationPromoterCMVAvailable sinceDec. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
mEos2-Dectin1A-C-10
Plasmid#57367PurposeLocalization: Membrane, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertDectin1A (CLEC7A Human)
UseTagsmEos2ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
mEos2-Dectin1A-N-10
Plasmid#57368PurposeLocalization: Membrane, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertDectin1A (CLEC7A Human)
UseTagsmEos2ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHL1575
Plasmid#53028PurposePconNoHind:csrB, PLtetO-1:RBS(st3):csrDDepositorInsertscsrB
csrD
UseSynthetic BiologyTagsRBS (st3)ExpressionBacterialMutationPromoterPconNoHind and pLtetO-1Available sinceMay 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
mPapaya1-Cx43-7
Plasmid#56649PurposeLocalization: Gap Junctions, Excitation: 530, Emission: 541DepositorInsertCx43
UseTagsmPapaya1ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pNH002
Plasmid#42145DepositorInsertN-TAP TAG::[(G4S)3]::mTFP1
UseTagsTAP TAGExpressionWormMutationPromoterAvailable sinceFeb. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pNH026
Plasmid#42148DepositorInsertmTFP1::linker::TAP TAG
UseTagsTAP-TagExpressionMutationPromoterAvailable sinceFeb. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pNH001
Plasmid#42144DepositorInsertTAP-tag
UseTagsTAP-tagExpressionWormMutationPromoterAvailable sinceFeb. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
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plenti.CMV.(cyto).iATPSnFR2.A95A.A119L.miRFP670nano3
Plasmid#236407PurposeExpresses cytosolic low affinity ratiometric ATP sensorDepositorInsert(cyto).iATPSnFR2.A95A.A119L.miRFP670nano3
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLUEF1 IRES GFP (backbone)
Plasmid#224466PurposeEf1a downstream of a ubiquitous chromatin opening element drives expression of GOI and IRES GFPDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterEf1aAvailable sinceApril 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pISEre1reRNA
Plasmid#226821PurposeExpressing ISEre1 guide RNA targeting maeB site controled by PJ23119DepositorInsertISEre1 none target guide RNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
GB4003
Plasmid#215248PurposeModule for the constitutive expression of PIF6 and PhyB:VP16DepositorInsertPIF6-PhyB:VP16
UseSynthetic BiologyTagsExpressionMutationV234L in PIF6- please see dep. sequence, BsaI and…PromoterAvailable sinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRSFDUET-1_VNp-anti-GFP mAb
Plasmid#214955PurposeBacterial expression of anti-GFP mAb light and heavy chain VNp fusions.DepositorInsertsVNp-anti-GFP mAb heavy chain
VNp-anti-GFP mAb light chain
UseTagsVNp6ExpressionBacterialMutationPromoterT7Available sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
EKAREN5-gl
Plasmid#225958PurposeThe YPet and mTurquoise sequence cassettes in EKAREN5(Addgene 167821) were replaced with a synonymous codon variant of YPet and mTurquoise-gl and subcloned into pCSII lentiviral backbone.DepositorInsertEKAREN5-gl
UseLentiviralTagsnls localization motifExpressionMammalianMutationPromoterEF-1-alpha promoterAvailable sinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
GB4653
Plasmid#215267PurposeTU for the constitutive expression of the flippase (FLP) recombinase with a nopaline synthase (Nos) promoterDepositorInsertpNos:FLP:tNos
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJan. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
GB4829
Plasmid#215275PurposeTU for the constitutive expression of the N. nambi NPGA gene under the P35S promoter and the TNOS terminatorDepositorInsertP35S:NPGA:TNOS
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJan. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-Zeo-miR-430
Plasmid#221726PurposeExpression of Zeocin resistance marker followed by perfect miR-430 target site insertion in zebrafish embryos.DepositorInsertBleoR-miR-430
UseFish expressionTagsExpressionMutationPromotersimian CMV IE94Available sinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
SBC015910
Plasmid#226287PurposeExpresses PhCFAT and PhIGS1 from trc promoter. Biosynthesis of isoeugenol from coniferyl alcohol.DepositorInsertsConiferyl alcohol acyltransferase
Isoeugenol synthase 1
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDIV302
Plasmid#204973PurposeAMA1 plasmid with Aspergillus optimized Mad7 and nat1 resistance markerDepositorInsertsMad7
nat1
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationPromoterAspergillus nidulans tef1 promoter and Aspergillu…Available sinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2-EcYRS-EGFP*
Plasmid#191118PurposeA plasmid for incorporation of O-Me-Tyr into an EGFP reporter for zebrafishDepositorInsertMutant E. coli TyrRS - T2A - enhanced green fluorescence protein
UseTagsHis tagExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSET_SynPspA
Plasmid#223812PurposeBacterial expression of SynPspA with N-terminal 10xHis-tag and EK cleavage siteDepositorInsertSynPspA
UseTags10xHis and NusAExpressionBacterialMutationPromoterAvailable sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBWB507
Plasmid#209325Purposep15A-tetR-tet-ME-RBS-mRFP-ME-U1-Barcode-U2-dbI terminator-oriT-Cm. Plasmid backbone for fragment expression.DepositorInsertmRFP
UseTagsExpressionBacterialMutationPromotertetAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACYC-yhaM
Plasmid#207650PurposeOverexpression of native L-cysteine desulfidase in E. coli MG1655DepositorInsertyhaM
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceOct. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACYC-decR
Plasmid#207653PurposeOverexpression of L-cysteine desulfidase transcriptional regulator in E. coli MG1655DepositorInsertdecR
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMF2-yhaO
Plasmid#207654PurposeOverexpression of L-cysteine importer in E. coli MG1655DepositorInsertyhaO
UseTags6xHisExpressionBacterialMutationPromoteraraBpAvailable sinceOct. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
GB3774
Plasmid#215247PurposeCDS of CPH from N. nambi codon optimized for N.benthamiana. This gene is the final step and recycling of the bioluminiscente pathwayDepositorInsertCPH
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only