We narrowed to 14,478 results for: NTS;

1. pJG363

   Plasmid

   #91183

   Purpose

   BeYDV replicon T-DNA for gene targeting in tobacco leaves, D10A single nickase (nAtCas9_D10A+gNt_R2+donor)

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   nAtCas9_D10A+gNt_R2+donor
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJG370

   Plasmid

   #91187

   Purpose

   BeYDV replicon T-DNA for gene targeting in tobacco leaves, D10A single nickase no gRNA control (nAtCas9_D10A+gRNActrl+donor)

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   nAtCas9_D10A+gRNActrl+donor
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-TRE-DIO-eYFP-f

   Plasmid

   #104057

   Purpose

   An AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein eYFP with a C-terminal Hras farnesylation sequence

   Depositor

   Viviana Gradinaru

   Article

   Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

   Insert

   EYFP-f
   

   Use

   AAV

   Promoter

   TRE

   Available Since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMOD_C2813

   Plasmid

   #91092

   Purpose

   Module C, Promoter: PvUbi2, Gene: Csy4 (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Csy4 (+ BaeI site for GT donor cloning)
   

   Use

   CRISPR

   Promoter

   PvUbi2

   Available Since

   July 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUSE-Src-KD-UniRapR-mCerulean-myc

   Plasmid

   #45382

   Depositor

   Klaus Hahn

   Article

   Dagliyan et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

   Insert

   Src-KD-UniRapR-mCerulean-myc
   

   Tags

   mCerulean and myc

   Expression

   Mammalian

   Mutation

   mSrc R388D (kinase dead), Y529F

   Promoter

   CMV

   Available Since

   Aug. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGWB419

   Plasmid

   #74813

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with 10xMyc (no promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   July 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3 WT MYOC eGLuc2 FLAG

   Plasmid

   #192820

   Purpose

   Expresses wild-type myocilin with a C-terminal enhanced Gaussia luciferase and FLAG tag

   Depositor

   John Hulleman

   Article

   Nakahara et al Curr Eye Res. 2022 Jun;47(6):918-922. do

   Insert

   myocilin (MYOC Human)
   

   Tags

   FLAG and enhanced Gaussia luciferase (M43I/M110I)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMOD_B2517

   Plasmid

   #91074

   Purpose

   Module B, Promoter: TaU3, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol III

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Esp3I ccdb cassette for single gRNA spacer cloning
   

   Use

   CRISPR

   Promoter

   TaU3

   Available Since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGWB522

   Plasmid

   #74864

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with GST (no promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   Sept. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. 373_MLH1 in pmiRGlo

    Plasmid

    #78133

    Purpose

    luciferase reporter for miRNA activity

    Depositor

    Heidi Schwarzenbach

    Article

    Eichelser et al Oncotarget. 2014 Oct 30;5(20):9650-63.

    Insert

    MLH1 (MLH1 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    3'UTR containing miRNA binding sites

    Promoter

    PGK

    Available Since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGL3-Basic-hCYP26A1P-E4-luciferase

    Plasmid

    #135592

    Purpose

    Short form DNA fragment of human CYP26A1gene promoter (E4) in pGL3-Basic-luc vector

    Depositor

    Catharine Ross

    Article

    Zhang et al Gene. 2010 Sep 15;464(1-2):32-43. doi: 1

    Insert

    Short form promoter of human CYP26A1 gene (E4)
    

    Promoter

    Human CYP26A1 promoter (Short Form, E4)

    Available Since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. mPlum-Gamma-Tubulin-17

    Plasmid

    #55971

    Purpose

    Localization: Centrosomes, Excitation: 590, Emission: 649

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    Gamma-Tubulin (TUBG1 Human)
    

    Tags

    mPlum

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

13. KHBD00233

    Plasmid

    #34377

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG7803 (z Fly)
    

    Use

    Gateway donor vector

    Available Since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

14. lentiCRISPR-v2-Blast-Puro

    Plasmid

    #167186

    Purpose

    Lentiviral expression of sgRNA with blasticidin and puromycin resistance genes

    Depositor

    Yin Shen

    Article

    Ren et al Sci Adv. 2021 Sep 17;7(38):eabi4360. doi

    Insert

    Blasticidin S deaminase
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMOD_C2806

    Plasmid

    #91091

    Purpose

    Module C, Promoter: FMV 34S, Gene: Csy4 (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Csy4 (+ BaeI site for GT donor cloning)
    

    Use

    CRISPR

    Promoter

    FMV 34S

    Available Since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBS-I-Sce1-slc6a3[11kb]:egfp

    Plasmid

    #53240

    Purpose

    allow visualization of pretectal dopaminergic neurons in vivo from day 3 post-fertilization and allow expression of transgenes in these neurons through adulthood

    Depositor

    Edward Burton

    Article

    Bai et al Neuroscience. 2009 Dec 15;164(3):1138-51

    Insert

    DAT-GFP
    

    Use

    Cloning

    Tags

    GFP

    Available Since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJFRC210-10XUAS-FRT>STOP>FRT-myr::smGFP-OLLAS

    Plasmid

    #63170

    Purpose

    GAL4-responsive UAS “flp-On” spaghetti monster GFP-OLLAS reporter

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

    Insert

    "spaghetti monster GFP-OLLAS"
    

    Tags

    N-myristoylation

    Expression

    Insect

    Available Since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. LentiU6-hNEUROD1 gRNA_1-MS2-Puro

    Plasmid

    #192676

    Purpose

    Lentiviral expression of sgRNA targeting hNEUROD1 promoter to activate human NEUROD1 transcription

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    Human NEUROD1 activating gRNA #1 (NEUROD1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAtCas9-D10A_2

    Plasmid

    #91160

    Purpose

    Cas9 only expression, Cas9 Type: AtCas9_D10A (nickase), Plant Selection: 2x35S:npt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    AtCas9_D10A (nickase)
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    D10A

    Available Since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET42b-A3A-PBE-ΔUGI

    Plasmid

    #119771

    Purpose

    To express the recombinant APOBEC3A in vitro

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

    Insert

    APOBEC3A-nCas9-NLS
    

    Tags

    6*HIS and NLS

    Expression

    Bacterial

    Mutation

    nCas9 has a mutation D10A in wild type SpCas9

    Promoter

    T7 promoter

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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