We narrowed to 15,864 results for: cas9

Showing: 6081 - 6100 of 15864 results

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pT7sgRNA

Plasmid

#111820

Purpose

Expresses sgRNA in T. brucei cells

Depositor

David Horn

Publication

Rico et al Sci Rep. 2018 May 21;8(1):7960. doi: 10.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

sgRNA

Use

CRISPR

Available since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pYZ155

Plasmid

#98411

Purpose

Pombe Expression Vector - nmt1 promoter with Sp.ura4 marker

Depositor

Jef Boeke

Publication

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Type

Empty backbone

Expression

Yeast

Available since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pKER-mKOk

Plasmid

#85975

Purpose

Expresses mKOk from the EF1a promoter in mammalian cells

Depositor

Michele Calos

Publication

Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mKOk

Expression

Mammalian

Promoter

EF-1a

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pJAT31

Plasmid

#204291

Purpose

For inserting a phiC31 attP site. HDR integrant is marked with ie1-DsRed, which is expressed in abdomen and mouthparts. Second U6 promoter allows use of two different gRNAs in two synthesized arms.

Depositor

David Stern

Publication

Stern et al bioRxiv 2023.06.17.545412

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ie1-DsRed

Use

CRISPR

Promoter

ie1

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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L40C-CRISPR.EFS.PAC

Plasmid

#89393

Purpose

Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA (hU6), PAC (Puromycin resistance) coexpression, EFS Promoter driven

Depositor

Dirk Heckl

Publication

Reimer et al Haematologica. 2017 Jun 1. pii: haematol

Type

Empty backbone

Use

CRISPR and Lentiviral

Available since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCfB3041(gRNA X-3)

Plasmid

#73283

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site X-3

Depositor

Irina Borodina

Publication

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

guiding RNA

Use

CRISPR; Grna

Expression

Yeast

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCfB3044(gRNA XI-2)

Plasmid

#73286

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XI-2

Depositor

Irina Borodina

Publication

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

guiding RNA

Use

CRISPR; Grna

Expression

Yeast

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCfB3046(gRNA XI-5)

Plasmid

#73288

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XI-5

Depositor

Irina Borodina

Publication

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

guiding RNA

Use

CRISPR; Grna

Expression

Yeast

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCfB3047(gRNA XII-1)

Plasmid

#73289

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-1

Depositor

Irina Borodina

Publication

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

guiding RNA

Use

CRISPR; Grna

Expression

Yeast

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pICSL90002

Plasmid

#68261

Purpose

Level 0 Golden Gate Part: Promoter U6-26 (Arabidopsis thaliana)

Depositor

Nicola Patron

Publication

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter: U6-26 (Arabidopsis thaliana)

Use

Synthetic Biology

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pYTK-DN6

Plasmid

#180287

Purpose

HygR multi-gene yeast expression vector (GFP-dropout)

Depositor

Andreas Milias-Argeitis

Publication

Novarina et al STAR Protoc. 2022 Jun 10;3(2):101358. do

Insert

ConLS' - GFP - ConRE' - HygR - CEN6/ARS4

Expression

Bacterial and Yeast

Available since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pUC19, containing H2-Kb c57/BL6 WK9

Plasmid

#102641

Purpose

Encodes sequence of the murine H2-Kb allele

Depositor

Sai Reddy

Publication

Kelton et al Sci Rep. 2017 Apr 4;7:45775. doi: 10.103

Insert

H2-Kd Balb/c

Available since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pDD372

Plasmid

#91824

Purpose

Codon-optimized GFP donor for the SapTrap cloning system

Depositor

Bob Goldstein

Publication

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

GFP-C1

Use

CRISPR

Expression

Worm

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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th-P2A-Gal4 donor

Plasmid

#65563

Purpose

Intron targeting-mediated Gal4 knockin at the zebrafish th locus.

Depositor

Jiu-lin Du

Publication

Li et al Cell Res. 2015 Apr 7. doi: 10.1038/cr.20

Insert

th-P2A-Gal4 (th Zebrafish)

Use

Zebrafish injection

Promoter

none

Available since

June 4, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGY18

Plasmid

#221708

Purpose

Empty Cas9 vector for multiplex gene editing in fungi

Depositor

Kyle Pomraning

Publication

Yuan et al Front Bioeng Biotechnol. 2024 Oct 16;12:

Type

Empty backbone

Use

CRISPR; Aspergillus

Expression

Bacterial

Available since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pRGEB34

Plasmid

#158152

Purpose

Construction of inPTG-Cas9 plasmids with a truncated 5'-UTR intron for plant genome editing

Depositor

Kabin Xie

Publication

Ding et al Mol Plant. 2018 Apr 2;11(4):542-552. doi

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pEG302 22aa SunTag NtDRMcd (no NLS) SUP

Plasmid

#115490

Purpose

CRISPR-Cas9 SunTag system to target NtDRMcd (without an NLS) to the SUPERMAN locus with two guide RNAs

Depositor

Steven Jacobsen

Publication

Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

Insert

g2_U6_g1_U6_NOS_NLS_GB1_noNLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS

Use

CRISPR and Synthetic Biology

Expression

Plant

Available since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-dSa VP64 Neurog2

Plasmid

#99695

Purpose

Expresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Neurog2 promoter, vector allows for activation of mouse Neurog2, can be packaged and delivered as AAV

Depositor

George Church

Publication

Vora et al bioRxiv 2018. 298620

Insert

dCas9 and gRNA targeting Neurog2 (gRNA: GGTATATAAGGGGTTTTAAG) (Neurog2 Mouse, Synthetic)

Use

AAV and CRISPR

Tags

VP64

Mutation

dead Cas9

Available since

Sept. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pYTK-DN3

Plasmid

#180284

Purpose

Spacer cassette (TU2)

Depositor

Andreas Milias-Argeitis

Publication

Novarina et al STAR Protoc. 2022 Jun 10;3(2):101358. do

Insert

ConL1 - Spacer - ConR2

Expression

Bacterial

Available since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCPH2A.X_S139D-GS3

Plasmid

#204748

Purpose

Expression of Cas9 and human H2AX with the S139D mutation

Depositor

Yuichiro Miyaoka

Publication

Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

H2AX S139D (H2AX Human)

Expression

Mammalian

Mutation

H2AX with the S139D mutation

Available since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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