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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 146,921 results for: ins;

Showing: 6101 - 6120 of 146921 results

  1. EBA140-bio

    Plasmid
    #47742
    Purpose
    Expresses enzymatically monobiotinylated full-length EBA140 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised EBA140
    Use
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available Since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47742-plasmid-map-sequence-id-68750

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  2. YW249_Pzipt17-loxP-H2BGFP-miniMOS

    Plasmid
    #196063
    Purpose
    a plasmid modified from miniMOS vector pCFJ910. the modification including insertions of SL2::H2B::GFP downstream to NeoR CDS and 2 loxP sites flanking the NeoR and GFP cassettes.
    Depositor
    Wenxing Yang
    Article
    Li et al PeerJ. 2023 May 17;11:e15433. doi: 10.77
    Inserts
    H2B::GFP
    NeoR
    Minimal Mos1
    zipt17p::zipt17::zipt17 3UTR
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available Since
    Jan. 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    196063-plasmid-map-sequence-id-383077

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  5. p-mCherry-C1-iCoChR++

    Plasmid
    #98173
    Purpose
    artificial anion conducting channelrhodopsin (aACR). Activation with blue light. Codon optimized for mammalian expression.
    Depositor
    Peter Hegemann
    Article
    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.
    Insert
    Synthetic construct iCoChR++ gene
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    A59S, E83N, E90Q, E101S, Q117R, E123S, V242R, T24…
    Promoter
    CMV (+enhancer)
    Available Since
    July 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98173-plasmid-map-sequence-id-182757

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  7. pSEM416 - mosTI unc-119 P_UPN - gfp(nls) - tbb-2

    Plasmid
    #182359
    Purpose
    Positive control for mosTI(unc-119). unc-119 rescue and pan-neuronal nuclear GFP expression after mosTI insertion.
    Depositor
    Christian Froekjaer-Jensen
    Article
    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068
    Insert
    Ultra-pan-neuronal positive control pUNP::gfp(NLS)
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Promoter Ultra Pan Neuronal
    Available Since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182359-plasmid-map-sequence-id-361684

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  10. pCAG-hMb3Cas12a-NLS(nucleoplasmin)-3xHA (RTW2500)

    Plasmid
    #115142
    Purpose
    CAG promoter expression plasmid for human codon optimized Mb3Cas12a nuclease with C-terminal NLS and HA tag
    Depositor
    Joseph Bondy-Denomy, Benjamin Kleinstiver, Keith Joung
    Article
    Marino et al Science. 2018 Sep 6. pii: science.aau517
    Insert
    human codon optimized Mb3Cas12a
    Use
    Tags
    NLS(nucleoplasmin) and 3xHA
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    115142-plasmid-map-sequence-id-220502

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  12. pAAV-hSyn-STChRger2-TS-EYFP

    Plasmid
    #129395
    Purpose
    Soma Targeted, High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by human Synapsin I promoter.
    Depositor
    Viviana Gradinaru
    Article
    Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s
    Insert
    soma targeted ChRger2
    Use
    AAV
    Tags
    Kv2.1-TS-EYFP
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available Since
    Oct. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    129395-plasmid-map-sequence-id-258248

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  18. pDisplay-EGFP-2A-HA-Nrxn3-alpha- 2(-)/3(+)/4(+)/6(+)

    Plasmid
    #184334
    Purpose
    Expression of mouse Nrx3-alpha lacking insertion at the alternative splice site 4 together with EGFP protein separated by 2A peptide. Contains insertions at alternatively splice sites 1,2,3 and 6.
    Depositor
    Peter Scheiffele
    Article
    Hauser et al Neuron. 2022 May 8. pii: S0896-6273(22)0
    Insert
    Nrxn3-alpha (Nrxn3 Mouse)
    Use
    Tags
    HA-tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    June 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    184334-plasmid-map-sequence-id-365498

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  19. Pf113-bio

    Plasmid
    #47729
    Purpose
    Expresses enzymatically monobiotinylated full-length Pf113 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised Pf113
    Use
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available Since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47729-plasmid-map-sequence-id-68462

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Showing: 6101 - 6120 of 146921 results