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tdTurboRFP-Mito-7

Plasmid

#58062

Purpose

Localization: Mitochondria, Excitation: 553, Emission: 576

Depositor

Michael Davidson

Insert

Mito (COX8A Human)

Tags

tdTurboRFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 4, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pDONR221-SLC18A2_STOP

Plasmid

#161163

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC18A2 (SLC18A2 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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SypHer mt

Plasmid

#48251

Purpose

pH sensor, targeted to mitochondria

Depositor

Nicolas Demaurex

Article

Poburko et al J Biol Chem. 2011 Apr 1;286(13):11672-84

Insert

SypHer

Tags

2 mitochondrial targeting sequences

Expression

Mammalian

Mutation

C199S

Promoter

CMV

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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Human PNP his-tagged

Plasmid

#64076

Purpose

Expression of human Purine Nucleoside Phosphorylase

Depositor

Vern Schramm

Article

Lewandowicz et al J Biol Chem. 2003 Aug 22;278(34):31465-8

Insert

Purine Nucleoside Phosphorylase (PNP Human)

Tags

Histidine tag

Expression

Bacterial

Available Since

April 29, 2015

Availability

Academic Institutions and Nonprofits only
pGWB502

Plasmid

#74844

Purpose

Gateway cloning compatible binary vector for expression of gene by CaMV35S promoter.

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-flex-lck-smV5

Plasmid

#196423

Purpose

Cre-dependent AAV expression of membrane-targeted V5 spaghetti monster reporter; no cell-type specificity

Depositor

Stanley Thomas Carmichael

Article

Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

Insert

Lck-smV5

Use

AAV and Cre/Lox

Tags

Lck

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pTRIP-CMV-tagRFP-FLAG-cGAS

Plasmid

#86676

Purpose

Expresses catalytically-active tagRFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events.

Depositor

Nicolas Manel

Article

Raab et al Science. 2016 Apr 15;352(6283):359-62. d

Insert

tagRFP-FLAG-cGAS

Use

Lentiviral

Available Since

April 18, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC15A1

Plasmid

#131874

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC15A1 (SLC15A1 Human)

Expression

Mammalian

Available Since

Oct. 9, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pCLIPf-NK1R Control Plasmid

Plasmid

#101125

Purpose

Expression of CLIP-tagged Neurokinin-1 Receptor in Mammalian cells

Depositor

New England Biolabs, Ana Egana

Insert

Neurokinin-1 Receptor, Truncated (TACR1 Human)

Tags

10xHis, CLIP-tag (CLIPf), and FLAG Epitope

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pH3U3-mcs

Plasmid

#12609

Depositor

Scot Wolfe

Article

Meng et al Nat Biotechnol. 2005 Aug . 23(8):988-94.

Insert

Multiple cloning site in His3 Ura3 reporter plasmid

Expression

Bacterial

Available Since

Sept. 26, 2006

Availability

Academic Institutions and Nonprofits only

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pSuperRetropuro-IL-6 shRNA 1

Plasmid

#19959

Depositor

Christopher Counter

Article

Ancrile et al Genes Dev. 2007 Jul 15. 21(14):1714-9.

Insert

pSuperRetropuro-IL-6 shRNA 1 (IL6 Human)

Use

RNAi and Retroviral

Available Since

Jan. 12, 2009

Availability

Academic Institutions and Nonprofits only
pSLQ9824: CMV-SV40_NLS-CasMINI-V3.1--3xFlag- c-Myc_NLS-polyA

Plasmid

#176271

Purpose

Expression of CasMINI-V3.1 in mammalian cells.

Depositor

Stanley Qi

Article

Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

Insert

CasMINI-V3.1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Puro-mStayGold

Plasmid

#227250

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-mStayGold cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pFC332

Plasmid

#87845

Purpose

AMA1 plasmid with Aspergillus optimized Cas9 and hph selection marker

Depositor

Uffe Mortensen

Article

Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

Inserts

Cas9
hph (hygromycin resistance marker)

Use

CRISPR; Fungal expression

Tags

SV40 NLS

Expression

Bacterial

Promoter

Aspergillus nidulans tef1 promoter and Aspergillu…

Available Since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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pX-RCC1-HA

Plasmid

#106938

Purpose

Transient expression of HA-tagged RCC1 in mammalian cells (CMV promoter)

Depositor

Patrizia Lavia

Article

DeLuca et al Cancer Res. 2003 Mar 15;63(6):1430-7.

Insert

human RCC1-HA (RCC1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV promoter

Available Since

March 6, 2018

Availability

Academic Institutions and Nonprofits only

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pRDA174_EF1a_EnAsCas12a_BFP

Plasmid

#196724

Purpose

Sortable and selectable EnAsCas12a.

Depositor

Bernhard Schmierer

Insert

enASCas12a-HA-T2A-BSD-TagBFP

Use

CRISPR and Lentiviral

Tags

Nucleoplasmin NLS - HA and SV40 NLS

Mutation

E174R, S542R, K548R

Promoter

EF1a

Available Since

April 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV5B-Flag-Smurf2 wt

Plasmid

#11746

Depositor

Jeff Wrana

Article

Kavsak et al Mol Cell. 2000 Dec . 6(6):1365-75.

Insert

SMURF2 (SMURF2 Human)

Tags

Flag

Expression

Mammalian

Available Since

May 12, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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pAJM.336

Plasmid

#108528

Purpose

LacIAM + PTac-YFP Reporter. IPTG sensor for use in non-Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

LacIAM + PTac-YFP

Expression

Bacterial

Mutation

LacI Q18M A47V F161Y

Available Since

July 27, 2018

Availability

Academic Institutions and Nonprofits only

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pSuperRetropuro-IL-6 scramble

Plasmid

#19960

Depositor

Christopher Counter

Article

Ancrile et al Genes Dev. 2007 Jul 15. 21(14):1714-9.

Insert

pSuperRetropuro-IL-6 scramble

Use

RNAi and Retroviral

Available Since

Jan. 12, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3-luxGold

Plasmid

#229948

Purpose

Mammalian expression of a de novo designed, FRET-based neoLux1.2-mGold fusion bioluminescent probe (max. Em=530nm)

Depositor

Andy Yeh

Article

Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

Insert

luxGold

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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