164,802 results

1. pcDNA3.0 FLAG TRPV4

   Plasmid

   #45751

   Depositor

   Robert Lefkowitz

   Article

   Shukla et al J Biol Chem. 2010 Sep 24;285(39):30115-2

   Insert

   TRPV4 (Trpv4 Rat)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 21, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. MSCV-opEBNA2-IRES-GFP

   Plasmid

   #220354

   Purpose

   Expresses EBV latent gene EBNA2 in mammalian cells (EBNA2 coding sequence was optimised for expression and carries a C-terminal 3xHA-tag)

   Depositor

   Klaus Rajewsky

   Article

   Zhang et al Front Immunol. 2023 Dec 19;14:1331730. d

   Insert

   opEBNA2 (EBNA-2 Epstein-Barr Virus (EBV) strain B95-8)
   

   Use

   Retroviral

   Tags

   3 x HA tag

   Expression

   Mammalian

   Mutation

   codon-optimised for expression in mammalian cells

   Promoter

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCAG mEmerald-EB3

   Plasmid

   #168514

   Purpose

   Expression of human plus-TIP microtubule binding protein EB3 fused with mEmerald for microtubule +end live tracking

   Depositor

   Franck Polleux

   Article

   Lewis TL et al Curr Biol. 2016 Oct 10;26(19):2602-2608.

   Insert

   EB3 (MAPRE3 Human)
   

   Use

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   May 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV3Tag8li-hHP1BP3-HA

   Plasmid

   #165468

   Purpose

   Human HP1BP3 mammalian expression vector with C-terminal HA tag.

   Depositor

   Martin Dorf

   Article

   Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

   Insert

   Heterochromatin protein 1 binding protein 3 (HP1BP3 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHR-CMV-TetO2_3C-Twin-Strep_IRES-EmGFP

   Plasmid

   #113884

   Purpose

   Mammalian (inducible) protein expression, bicistronic expression of cytosolic EmGFP

   Depositor

   A. Radu Aricescu

   Article

   Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3C-Twin-Strep

   Expression

   Mutation

   Promoter

   pHR-CMV-TetO2

   Available Since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. FLX1.8NFATGFPpd2HS4

   Plasmid

   #169096

   Purpose

   Lentiviral vector encoding a short-lived GFP under the transcriptional control of a mutlimerized NFAT gene enhancer to measure T cell activation

   Depositor

   Geoffrey Owens

   Inserts

   NFAT enhancer (from IL-4 promoter)
   Destabilized green fluorescent protein
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   NFAT enhancer + synthetic TATA box

   Available Since

   July 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV hSyn FLEx mGFP-2A-Synaptophysin-mRuby

   Plasmid

   #71760

   Purpose

   mGFP and Synaptophysin-mRuby expression from Cre-expressing neurons

   Depositor

   Liqun Luo

   Article

   Beier et al Cell. 2015 Jul 30;162(3):622-34. doi: 10

   Has Service

   AAV1

   Inserts

   mGFP
   Synaptophysin
   

   Use

   AAV and Cre/Lox

   Tags

   Palmitoylation signal and mRuby

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.1-20nm-EBFP2

   Plasmid

   #231185

   Purpose

   Expresses a synthetic linker, tagged with EBFP2, Stabilizing the ER-mitochondrial distance at 20 nm

   Depositor

   Dmitry Lim

   Article

   Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

   Insert

   20nm-EBFP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AAV-EF1A-DIO-PSD95.FingR-eGFP-CCR5TC

   Plasmid

   #126216

   Purpose

   Labeling of excitatory synapses (green fluorescence) in cre-expressing neurons

   Depositor

   Xue Han

   Article

   Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

   Insert

   PSD95.FingR-eGFP-CCR5TC
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available Since

   July 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMVTNT-T7-KPNA5

    Plasmid

    #26681

    Depositor

    Bryce Paschal

    Article

    Kelley et al BMC Cell Biol. 2010 Aug 11;11:63. doi: 1

    Insert

    Karyopherin Alpha 5 (KPNA5 Human)
    

    Use

    Tags

    T7 (MASMTGGQQMG)

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 10, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. HA-HIF2alpha-P405A/P531A-pcDNA3

    Plasmid

    #18956

    Depositor

    William Kaelin

    Article

    Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

    Insert

    Hypoxia inducible factor 2 alpha (EPAS1 Human)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    cDNA changed amino acids: Proline 405 to Alanin…

    Promoter

    Available Since

    Aug. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV FLEX Synaptophysin GFP

    Plasmid

    #137188

    Purpose

    Cre-dependent expression of synaptophysin-GFP

    Depositor

    Matthew Nolan

    Article

    Surmeli et al Neuron. 2016 Nov 23;92(4):929. doi: 10.1

    Insert

    Synaptophysin GFP (Syp Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCI-neo beta catenin S33Y

    Plasmid

    #16519

    Depositor

    Bert Vogelstein

    Article

    Morin et al Science. 1997 Mar 21. 275(5307):1787-90.

    Insert

    beta catenin (CTNNB1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S33Y (SW48 mutation)

    Promoter

    Available Since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDNR-mCherry-T2A-3xPuroR-Frame 0

    Plasmid

    #206991

    Purpose

    Donor plasmid for Frame 0 with mCherry tag and triple Puromycin resistant marker (3xPuroR)

    Depositor

    Alejandro Chavez

    Article

    Kim et al Sci Adv. 2024 May 3;10(18):eadg8771. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available Since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTW040

    Plasmid

    #64769

    Purpose

    For integration of Cre recombinase at the yeast HIS3 locus orthe CYC1 terminator

    Depositor

    Fred van Leeuwen

    Article

    Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

    Insert

    Cre-EBD78
    

    Use

    Tags

    fused to the human Estrogen Binding Domain (EBD),…

    Expression

    Yeast

    Mutation

    Promoter

    TDH3

    Available Since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTHSSe_45

    Plasmid

    #109243

    Purpose

    pSC101 var3 BbsI cloning vector

    Depositor

    Christopher Voigt

    Article

    Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PJ23105

    Available Since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1+DPP6

    Plasmid

    #117272

    Purpose

    Encodes human DPP6 variant 1

    Depositor

    Mark Cannell

    Article

    Lainez et al Biochem Pharmacol. 2018 Apr;150:120-130.

    Insert

    DPP6 (DPP6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AAV-CAG-FLPX-rc [ChrimsonR-tdTomato]

    Plasmid

    #130909

    Purpose

    AAV-mediated expression of ChrimsonR-tdTomato under the CAG promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Has Service

    AAV8

    Insert

    ChrimsonR-tdTomato
    

    Use

    AAV

    Tags

    tdTomato (codon diversified version)

    Expression

    Mammalian

    Mutation

    Chrimson K176R mutant

    Promoter

    CAG

    Available Since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pIVM_02

    Plasmid

    #204500

    Purpose

    Expression of VHL (54-213) for production of VCB complexes in conjunction with plasmid pIVM_26 (EloB 1-104, EloC 17-112)

    Depositor

    Alessio Ciulli

    Article

    Cardote et al Structure. 2017 Jun 6;25(6):901-911.e3.

    Insert

    amino acids 54-213 VHL (VHL Human)
    

    Use

    Tags

    6xHis, TEV, and Thrombin

    Expression

    Bacterial

    Mutation

    0

    Promoter

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Flag-neo-PP1-Gamma

    Plasmid

    #171270

    Purpose

    Mammalian expression of 3x-Flag-tagged human PP1 gamma catalytic subunit isoform 1

    Depositor

    Steven Balk

    Insert

    3x-Flag-tagged human PP1 gamma catalytic subunit isoform 1 (PPP1CC Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge