159,557 results

1. EFS-EGFP-P2A-Neo-WPRE-U6-AsCas12aDR-AsCas12aDR (pRG212)

   Plasmid

   #149722

   Purpose

   Lentiviral expression of crRNA with GFP and a neomycin resistance gene

   Depositor

   Junwei Shi

   Article

   Gier et al Nat Commun. 2020 Jul 13;11(1):3455. doi:

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter for crRNA expression and EFS promoter…

   Available since

   June 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLX307 P2A GFP YAP1

   Plasmid

   #193675

   Purpose

   Constitutive lentiviral expression of YAP1

   Depositor

   William Hahn

   Article

   Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

   Insert

   YAP1 (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. TFORF2538

   Plasmid

   #144036

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   SOX4 (SOX4 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-hAR1-660

   Plasmid

   #89079

   Purpose

   mammalian expression of human androgen receptor N-terminal fragment 1-660 amino acids

   Depositor

   Elizabeth Wilson

   Article

   Simental et al J Biol Chem. 1991 Jan 5;266(1):510-8.

   Insert

   hAR-1-660 (AR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   aa 1-660 (contains exons A,B,C and part of D)

   Promoter

   CMV

   Available since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. p7058 pHAGE-P-CMVt-N-HA-GAW-FDFT1

   Plasmid

   #100151

   Purpose

   Expresses FDFT1

   Depositor

   Peter Howley

   Article

   Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

   Insert

   FDFT1 (FDFT1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. lenti-sgRNA hygro

   Plasmid

   #104991

   Purpose

   This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and hygromycin resistance from an EF-1a promoter.

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUCmini-iCAP-AAV.MaCPNS2

   Plasmid

   #185137

   Purpose

   non-standard AAV2 rep-AAV-MaCPNS2 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

   Depositor

   Viviana Gradinaru

   Article

   Chen et al Neuron. 2022 Jul 20;110(14):2242-2257.e6

   Insert

   Synthetic construct isolate AAV-MaCPNS2 VP1 gene
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   p41

   Available since

   June 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Str-KDEL_CD63-SBP-mCherry

   Plasmid

   #222326

   Purpose

   Synchronize the trafficking of CD63 from the ER.

   Depositor

   Franck Perez

   Article

   Mathieu et al Nat Commun. 2021 Jul 19;12(1):4389. doi:

   Insert

   Streptavidin-KDEL and CD63 fused to SBP-mCherry (CD63 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pILGFPB5A

   Plasmid

   #83556

   Purpose

   Used to evaluate the expression output of GAL1 promoter with yEGFP used as the reporter gene.

   Depositor

   Claudia Vickers

   Article

   Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

   Insert

   GAL1 promoter-yEGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   Dec. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-T7-SpRY-P2A-EGFP (RTW4830)

    Plasmid

    #139989

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9 variant named SpRY with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…

    Promoter

    CMV and T7

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. Flag-M4-AR-S81A

    Plasmid

    #171241

    Purpose

    Mammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant: AR-Ser81Ala (AR-S81A)

    Depositor

    Steven Balk

    Article

    Chen et al J Biol Chem. 2012 Mar 9;287(11):8571-83.

    Insert

    3xFlag-tagged human androgen receptor Ser81Ala mutant (AR Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    AR-Ser81Ala (AR-S81A)

    Promoter

    CMV

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5FRT/Flag-WDR5

    Plasmid

    #29759

    Depositor

    Joan Conaway

    Article

    Cai et al J Biol Chem. 2010 Feb 12. 285(7):4268-72

    Insert

    WDR5 (WDR5 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 29, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pET47b-T7-enAsCas12f

    Plasmid

    #204642

    Purpose

    Expression of enAsCas12f in E. coli

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

    Insert

    enAsCas12f
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    D196K, N199K, G276R, N328G, D364R

    Promoter

    T7

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. Ecoli cpGFP

    Plasmid

    #215713

    Purpose

    Expresses cpGFP in Ecoli

    Depositor

    Katharine Diehl

    Article

    Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

    Insert

    cpGFP
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    circularly permuted GFP

    Promoter

    T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCaggs-mGold2s

    Plasmid

    #231763

    Purpose

    Expression of mGold2t in mammalian cells

    Depositor

    Francois St-Pierre

    Article

    Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

    Insert

    mGold2s
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mGold2s is mGold with V1A;V22I;H77N;Q80R;K101E;D1…

    Promoter

    Actin promoter with CMV enhancer

    Available since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. NES-Grx1-roGFP2

    Plasmid

    #236551

    Purpose

    Expressing fluorescent Grx1-roGFP2 fusion protein in mammalian cells with localization in the cytoplasm.

    Depositor

    Pablo Rivera-Fuentes

    Insert

    NES-Grx1-roGFP2 (NES Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pR0306 CcaCas13b His6-TwinStrep-SUMO

    Plasmid

    #182687

    Purpose

    Expresses CcaCas13b with N terminal 6xHis-SUMO tag for recombinant bacterial expression and purification

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

    Insert

    CcaCas13b
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPB-CAG-ECC-Biosensor

    Plasmid

    #140832

    Purpose

    Encoding the CAG promoter based eccDNA biosensor

    Depositor

    Yonglun Luo

    Article

    Moller et al Nucleic Acids Res. 2018 Dec 14;46(22):e1

    Insert

    ECC-Biosensor
    

    Use

    Piggybac (pb) transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCL20C-ddIPpoI

    Plasmid

    #49053

    Purpose

    Expresses the restriction enzyme IPpoI under the control of Shield1

    Depositor

    Michael Kastan

    Article

    Goldstein et al Proc Natl Acad Sci U S A. 2013 Sep 30.

    Insert

    DD-HA-ER-IPpoI
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBE14

    Plasmid

    #174653

    Purpose

    In vivo Cre-lox circularization in E. coli

    Depositor

    Huimin Zhao

    Article

    Enghiad et al Nat Commun. 2021 Feb 19;12(1):1171. doi:

    Insert

    Cre
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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