We narrowed to 6,639 results for: itch
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Plasmid#219548PurposeNLS-Gal4(1-65)-iLight2-VP16-T2A-mTagBFP2 construct expression in mammalian cells.DepositorInsertNLS-Gal4(1-65)-iLight2-VP16-T2A-mTagBFP2
ExpressionMammalianMutationThe (SAGG)x4 linker between GAL4(1-65) and the PC…PromoterCMVd1Available SinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
SspB-iRFP-DHPH-TIAM1
Plasmid#176118PurposeHeterodimerization with iLID, Rac1 activationDepositorInsertTIAM1 (TIAM1 Human)
ExpressionMammalianAvailable SinceOct. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR-HNRNPA1C-mCh-Cry2WT
Plasmid#101226PurposehnRNPA1(186-320) fused to mCh-Cry2WTDepositorAvailable SinceFeb. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEntr1a-SspB-HaloTag-TIAM1-DHPH
Plasmid#176134PurposeHeterodimerization with iLID, Rac1 activationDepositorInsertTIAM1 (TIAM1 Human)
UseEntry vectorAvailable SinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2-CRY2-mCherry
Plasmid#140571PurposeFor use in light-induced protein inactivation through the interaction with CIB1DepositorAvailable SinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Solo-mScarlet-TUBA1B
Plasmid#207764PurposeDonor template for mScarlet insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763DepositorInsertTUBA1B Homology Arms flanking a mScarlet Tag (TUBA1B Human)
UseCRISPR; Donor templateExpressionMammalianAvailable SinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-anti-GFP(LaG2) PAGER(TF)
Plasmid#229996PurposeExpresses anti-GFP PAGER(TF) (high sensitivity) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)DepositorInsertIL2SP-Aro6-LaG2-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
UseAAVExpressionMammalianMutationV360A/R361A on KORDPromoterCMVAvailable SinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Solo-mStayGold-TUBA1B
Plasmid#227327PurposeDonor template for mStayGold insertion into the N-terminus of the TUBA1B locus. For tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B (Addgene #207763)DepositorInsertTUBA1B Homology Arms flanking a mStayGold Tag (TUBA1B Human)
UseCRISPR; Donor templateExpressionMammalianPromoterPromoterlessAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
KIF5A-mVenus-iLID
Plasmid#240422Purpose+end oriented kinesin for optogenetic pulling experimentsDepositorAvailable SinceJuly 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
CRY2 mCherry Sec61B
Plasmid#102246PurposeExpresses fusion of CRY2PHR with mCherry and Sec61BDepositorAvailable SinceNov. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR-attL5-CRY2-mCh-attL2
Plasmid#160438PurposeEntry vector for cloning Cryptochrome2-mCherry using 2-fragment gateway recombinationDepositorAvailable SinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
PR_GEF YFP (2XPDZ-YFP-LARG DH)
Plasmid#80408PurposeRecruitable YFP tagged RhoA GEF LARG (DH domain). Expresses in mammalian cellsDepositorAvailable SinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-mScarlet-Puro-H2BC11
Plasmid#207761PurposeDonor template for mScarlet-2A-Puro insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755DepositorInsertH2BC11 Homology Arms flanking a mScarlet-Puro Cassette (H2BC11 Human)
UseCRISPR; Donor templateExpressionMammalianAvailable SinceNov. 17, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-anti-mCherry(LaM8) PAGER(TF)
Plasmid#229999PurposeExpresses anti-mCherry PAGER(TF) (high reversibility) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)DepositorInsertIL2SP-Aro6-LaM8-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
UseAAVExpressionMammalianMutationV360A/R361A on KORDPromoterCMVAvailable SinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
LiPOP2
Plasmid#168266PurposeA single-component optogenetic tool for photoswitchable pyroptosisDepositorInsertmGSDMD(1-276) (Gsdmd Mouse)
TagscpLOV2, mCherryExpressionMammalianMutationR138A, K146A, R152A, R154APromoterCMVAvailable SinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
SnapTag-Binder
Plasmid#179142PurposeBinder: SspB that carries two point mutations reducing homodimerization(Y11K/A15E, residue numbering from pdb: 1ou9). Binder binds to the 7 a.a. peptide SsrA (AANDENY).DepositorInsertSspB
TagsSNAP-tagExpressionMammalianMutationY11K/A15E, residue numbering from pdb: 1ou9Available SinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
GANLS
Plasmid#50852PurposeExpresses one ddFP in mammalian cells, the second plasmid for a red to green colour switch-based fluorescent caspase-3 biosensor, used together with plasmid NESRA-DEVD-BNLS.DepositorInsertddGFP A
Tagstriplicated NLS sequence DPKKKRKVExpressionMammalianPromoterCMVAvailable SinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
RANLS
Plasmid#50843PurposeExpresses one ddFP in mammalian cells, the second plasmid for a green to red colour switch-based fluorescent caspase-3 biosensor, used together with plasmid GANES-DEVD-BNLS.DepositorInsertddRFP A
Tagstriplicated NLS sequence DPKKKRKVExpressionMammalianPromoterCMVAvailable SinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
Flag-mCherry-Binder
Plasmid#179139PurposeBinder: SspB that carries two point mutations reducing homodimerization(Y11K/A15E, residue numbering from pdb: 1ou9). Binder binds to the 7 a.a. peptide SsrA (AANDENY).DepositorInsertSspB
TagsFLAG and mCherryExpressionMammalianMutationY11K/A15E, residue numbering from pdb: 1ou9Available SinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-anti-VEGF(Nb35) PAGER(TF)
Plasmid#230000PurposeExpresses anti-VEGF PAGER(TF) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)DepositorInsertIL2SP-Aro6-Nb35-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
UseAAVExpressionMammalianMutationV360A/R361A on KORDPromoterCMVAvailable SinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only