We narrowed to 58,550 results for: tra

1. pcDNA3.1(+)-wt-TTF-1

   Plasmid

   #49989

   Purpose

   For transient transfection to express human wild type TTF-1 protein

   Depositor

   David Mu

   Article

   Runkle et al J Biol Chem. 2012 Aug 17;287(34):28790-8

   Insert

   TTF-1 (NKX2-1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. LB601: pMVP/PB/eGFP-DEST

   Plasmid

   #121870

   Purpose

   pMVP destination vector, empty PiggyBac transposon vector backbone w/ eGFP selection cassette

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Piggybac transposon, pmvp gateway destination vec…

   Expression

   Mammalian

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-HsSENP3

   Plasmid

   #71467

   Purpose

   Encodes human full length SENP3. Suitable for in vitro transcription/translation (T7).

   Depositor

   Jorge Eduardo Azevedo

   Article

   Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

   Insert

   SUMO1/sentrin specific peptidase 3 (SENP3 Human)
   

   Tags

   Hexahistidine

   Expression

   Bacterial

   Mutation

   Gene optimised for E. coli expression (Genscript)

   Promoter

   T7

   Available Since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSEM320 - sgRNA mosTI unc-119 array insertion

   Plasmid

   #159824

   Purpose

   Single copy or array insertion by MosTI using split unc-119 selection

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Insert

   sgRNA mosTI unc-119 array insertion
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTXB1.Tat(48-57)-Cre recombinase

   Plasmid

   #165042

   Purpose

   Expression of Tat(48-57) conjugated Cre recombinase for protein purification (Intein-chitin binding domain fussion)

   Depositor

   Dokyun Na

   Article

   Lee et al Commun Biol. 2021 Feb 15;4(1):205. doi:

   Insert

   Cre recombinase
   

   Tags

   Mxe Intein-Chitin binding domain and Tat(48-57)

   Expression

   Bacterial

   Available Since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-p3-PE(1152)-N-term

   Plasmid

   #187181

   Purpose

   p3-PE2-N-term(1152) in AAV2 backbone (ITRs)

   Depositor

   Gerald Schwank

   Article

   Bock et al Sci Transl Med. 2022 Mar 16;14(636):eabl

   Insert

   PE2 from plasmid #132775
   

   Use

   AAV

   Available Since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. LC201: pMVP/PB/Puro-DEST

   Plasmid

   #121874

   Purpose

   pMVP destination vector, empty PiggyBac transposon vector backbone w/ Puro selection cassette

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Piggybac transposon, pmvp gateway destination vec…

   Expression

   Mammalian

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLTR.gp140/EGFP.Revdelta38/DsRed

   Plasmid

   #115775

   Purpose

   HIV splicing reporter allows the assessment of the effect of LRAs on HIV-1 transcription and splicing.

   Depositor

   Damian Purcell

   Article

   Khoury et al J Vis Exp. 2019 Jan 22;(143). doi: 10.37

   Insert

   Envelope fused to EGFP (gp140-EGFP) and non-functional Rev protein fused to DsRed fluorescent protein (Rev-delta38-DsRed).
   

   Tags

   EGFP; dsReD

   Expression

   Mammalian

   Mutation

   gp140 uncleaved (1190-91aa, KR > TG; truncated…

   Promoter

   HIV-1 (LTR)

   Available Since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1 MYC

   Plasmid

   #176045

   Purpose

   Expresses Myc in Mammalian cells

   Depositor

   Linda Penn

   Article

   Tu et al Cancer Cell. 2018 Oct 8;34(4):579-595.e8

   Insert

   Myc
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-FLAG-RNMT

    Plasmid

    #112705

    Purpose

    Expresses N-terminally FLAG-tagged human RNMT in mammalian cells

    Depositor

    Daniel Schoenberg

    Article

    Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10

    Insert

    RNMT (RNMT Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDestattB_ubi:EGFP (pCM272)

    Plasmid

    #68339

    Purpose

    Multisite Gateway destination vector for phiC31-mediated transgenesis in zebrafish, harbors ubiquitin promoter (ubi)-driven EGFP.

    Depositor

    Christian Mosimann, Leonard Zon

    Article

    Mosimann et al Dev Dyn. 2013 Aug;242(8):949-63. doi: 10

    Insert

    EGFP
    

    Use

    Zebrafish transgenesis

    Promoter

    ubi

    Available Since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV_Tre3g_CalcireticulinSS_mTurquoise_D4-cameleon_REAch2_KDEL

    Plasmid

    #216253

    Purpose

    FRET-based calcium sensor located in the ER lumen, Tet-inducible

    Depositor

    Edward Avezov

    Article

    Crapart et al Proc Natl Acad Sci U S A. 2024 Mar 26;12

    Insert

    ER-targeted D4 second generation cameleon (FRET calcium sensor)
    

    Use

    Lentiviral

    Tags

    REAch and mTurquoise

    Promoter

    TRE3G

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-FLEX-NBL10

    Plasmid

    #69971

    Purpose

    Cre-conditionally expresses the anti-GFP Nanobody-Rpl10a fusion

    Depositor

    Jeffrey Friedman

    Article

    Ekstrand et al Cell. 2014 May 22;157(5):1230-42. doi: 1

    Insert

    NBL10
    

    Use

    AAV and Cre/Lox

    Tags

    HA

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEK39

    Plasmid

    #210599

    Purpose

    Reporter gene expression in B. dendrobatidis

    Depositor

    Lillian Fritz-Laylin

    Article

    Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

    Insert

    SpH2Bpro-Hshph-G4S-HsmRuby3-ScADH1ter
    

    Tags

    mRuby3

    Expression

    Bacterial and Yeast

    Promoter

    SpH2Bpro

    Available Since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. PAGFP_NS2B3

    Plasmid

    #195755

    Purpose

    Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

    Depositor

    Partha Mondal

    Article

    Varghese et al bioRxiv 2022.12.18.520514

    Insert

    Dengue (Type-2) NS2B3
    

    Tags

    PA-GFP

    Expression

    Mammalian

    Mutation

    No mutations

    Promoter

    CMV

    Available Since

    Feb. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. mbYFPQS

    Plasmid

    #80742

    Purpose

    membrane-targeted biosensor for monitoring changes in intracellular chloride

    Depositor

    Susan Amara, Susan Ingram

    Article

    Watts et al PLoS One. 2012;7(4):e35373. doi: 10.1371

    Insert

    YFPQS
    

    Tags

    palmitoylation signal sequence of neuromodulin

    Expression

    Mammalian

    Mutation

    H148Q (CAC to CAG) and V163S (GTG to AGC).

    Promoter

    CMV

    Available Since

    Aug. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3.1-CD63-(TEV)-tTA

    Plasmid

    #162597

    Purpose

    human CD63-TEV cleavage site-tTA

    Depositor

    Masaharu Somiya

    Article

    Somiya et al J Extracell Vesicles. 2021 Nov;10(13):e1

    Insert

    human CD63 (CD63 Human)
    

    Tags

    TEV protease cleavage site and tTA

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSEM272 - MCS mosTI Pmlc-2_GFP target

    Plasmid

    #159834

    Purpose

    Single copy or array insertion by MosTI using split Pmlc-2 GFP selection

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    MCS mosTI Pmlc-2_GFP target
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. Venus-rab7a

    Plasmid

    #234743

    Purpose

    Mammalian expression of late endosome marker Venus-rab7a

    Depositor

    Nevin Lambert

    Article

    Lan et al Traffic. 2012 Nov;13(11):1450-6. doi: 10

    Insert

    Venus-rab7a (Rab7 Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. FLAG-Venus-pLenti6

    Plasmid

    #36948

    Depositor

    William Kaelin

    Article

    Koivunen et al Nature. 2012 Feb 15;483(7390):484-8. doi

    Insert

    Venus (EYFP)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    -F46L/F64L/V68L/M153T/V163A/S175G

    Promoter

    CMV

    Available Since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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